2001
Lack of Proteasome Active Site Allostery as Revealed by Subunit-Specific Inhibitors
Myung J, Kim K, Lindsten K, Dantuma N, Crews C. Lack of Proteasome Active Site Allostery as Revealed by Subunit-Specific Inhibitors. Molecular Cell 2001, 7: 411-420. PMID: 11239469, DOI: 10.1016/s1097-2765(01)00188-5.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric RegulationAnimalsBinding SitesCattleCell DivisionCells, CulturedChymotrypsinCysteine EndopeptidasesEndopeptidasesEpoxy CompoundsHumansHydrolysisKetonesKineticsModels, BiologicalMultienzyme ComplexesProtease InhibitorsProteasome Endopeptidase ComplexProtein SubunitsRecombinant Fusion ProteinsSerineSubstrate SpecificityTransfectionConceptsProtein degradation assaysSubunit-specific inhibitorsProtein degradationDegradation assaysCellular proliferationChymotrypsin-like activityPeptidyl-glutamyl peptideEpoxyketone inhibitorsActive siteSuch interactionsInhibitorsAllosteryProteasomeSitesSubunitsInhibitionSubstrateActivityProliferationAssaysPeptidesOccupancy
1999
Proteasome inhibition by the natural products epoxomicin and dihydroeponemycin: Insights into specificity and potency
Kim K, Myung J, Sin N, Crews C. Proteasome inhibition by the natural products epoxomicin and dihydroeponemycin: Insights into specificity and potency. Bioorganic & Medicinal Chemistry Letters 1999, 9: 3335-3340. PMID: 10612595, DOI: 10.1016/s0960-894x(99)00612-5.Peer-Reviewed Original Research
1998
The Antiproliferative Agent Didemnin B Uncompetitively Inhibits Palmitoyl Protein Thioesterase †
Meng L, Sin N, Crews C. The Antiproliferative Agent Didemnin B Uncompetitively Inhibits Palmitoyl Protein Thioesterase †. Biochemistry 1998, 37: 10488-10492. PMID: 9671519, DOI: 10.1021/bi9804479.Peer-Reviewed Original ResearchConceptsPalmitoyl-protein thioesterase 1GTP-binding proteinsDynamic protein palmitoylationDidemnin BPalmitoyl-protein thioesterasePalmitoyl proteinsProtein palmitoylationMembrane associationBaculoviral systemMyristoyl-CoAProduct bindsHa-rasBiochemical supportProteinEnzymatic activityBindingInhibition assaysDepalmitoylationPalmitoylationThioesteraseKinetic analysisInhibitionBindsRegulationUncompetitive mode