2024
Bridge-like lipid transfer protein family member 2 suppresses ciliogenesis
Parolek J, Burd C. Bridge-like lipid transfer protein family member 2 suppresses ciliogenesis. Molecular Biology Of The Cell 2024, 35: br11. PMID: 38536441, PMCID: PMC11151097, DOI: 10.1091/mbc.e24-02-0065.Peer-Reviewed Original ResearchConceptsLipid transfer proteinsFamily member 2RPE-1 cellsSuppressed ciliogenesisTubular endosomal networkMembrane contact sitesNegative regulator of ciliogenesisRegulator of ciliogenesisDrosophila melanogaster</i>Evolutionary conserved proteinMember 2Primary cilium biogenesisRPE-1Endosomal networkGenetic interactionsTubular endosomesCilium biogenesisProtein familyStructure predictionContact sitesEndoplasmic reticulumDomain-containingPreweaning lethalityNegative regulatorCiliogenesis
2023
Cholesterol-dependent homeostatic regulation of very long chain sphingolipid synthesis
Kim Y, Mavodza G, Senkal C, Burd C. Cholesterol-dependent homeostatic regulation of very long chain sphingolipid synthesis. Journal Of Cell Biology 2023, 222: e202308055. PMID: 37787764, PMCID: PMC10547602, DOI: 10.1083/jcb.202308055.Peer-Reviewed Original ResearchConceptsPlasma membraneEpidermal growth factor receptor signalingMembrane lipid homeostasisGrowth factor receptor signalingAcute cholesterol depletionCellular cholesterol homeostasisPlasma membrane cholesterol contentCellular cholesterol levelsMembrane lipid packingSterol-responsive elementMembrane cholesterol contentProtein translationCytoplasmic leafletCholesterol depletionSphingolipid synthesisGolgi apparatusProtein pathwayResponsive elementLipid homeostasisRegulatory axisReceptor signalingLysosome membraneSphingomyelin synthesisAccumulation of cholesterolMetabolic axisLipid Sorting and Organelle Identity.
Kim Y, Burd C. Lipid Sorting and Organelle Identity. Cold Spring Harbor Perspectives In Biology 2023, 15: a041397. PMID: 37487627, PMCID: PMC10547387, DOI: 10.1101/cshperspect.a041397.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsTrafficking of lipidsSynthesis of sphingolipidsLipid transfer proteinOrganelle identityMembrane traffickingOrganelle homeostasisSoluble lipid transfer proteinsBulk membrane propertiesOrganelle functionGolgi networkLipid sortingNet membrane chargeOrganelle membranesPlasma membraneEndoplasmic reticulumParticular lipidsOrganellesTransfer proteinLeaflet asymmetryEssential roleBulk membraneCholesterol fluxTraffickingMembraneSecretory
2022
Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathway
Sokoya T, Parolek J, Foged M, Danylchuk D, Bozan M, Sarkar B, Hilderink A, Philippi M, Botto L, Terhal P, Mäkitie O, Piehler J, Kim Y, Burd C, Klymchenko A, Maeda K, Holthuis J. Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathway. ELife 2022, 11: e79278. PMID: 36102623, PMCID: PMC9531943, DOI: 10.7554/elife.79278.Peer-Reviewed Original ResearchConceptsSecretory pathwayER export signalPatient-derived fibroblastsFull enzymatic activityPhysical membrane propertiesExport signalSterol gradientLipid landscapeMammalian cellsCholesterol organizationLipid orderEnzymatic activityLipid distributionOsteogenic cellsPathwayBone phenotypeSMS2Glycerophospholipid profilePathogenic variantsCellsVariantsSphingomyelinSkeletal dysplasiaERSphingolipids
2019
Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus
Sundberg EL, Deng Y, Burd CG. Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus. Developmental Cell 2019, 51: 387-398.e4. PMID: 31543446, PMCID: PMC6832887, DOI: 10.1016/j.devcel.2019.08.014.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSphingomyelin-rich membranesTransmembrane domainSecretory pathwayVesicular transport carriersIntegral membrane proteinsProtein transmembrane domainBiosynthetic secretory pathwaySyndecan-1Heparan sulfate chainsLipoprotein lipaseSorting receptorSecretion pathwayMembrane proteinsGolgi membranesProteoglycan syndecan-1Protein cargoSecretory vesiclesPlasma membraneGolgi apparatusSpecific sequencesTransport carriersSulfate chainsLipid compositionEnzyme lipoprotein lipase
2018
Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network
Deng Y, Pakdel M, Blank B, Sundberg EL, Burd CG, von Blume J. Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Developmental Cell 2018, 47: 464-478.e8. PMID: 30393074, PMCID: PMC6261503, DOI: 10.1016/j.devcel.2018.10.012.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkTGN membranesCell surface transportProtein sortingTrafficking pathwaysSecretion pathwaySecretory proteinsSphingomyelin synthesisSorting mechanismLipid synthesisProteinVesicular carriersCab45Sphingomyelin contentNew lipidSortingPrincipal functionMembranePathwayCore componentLipidsGolgiSurface transportExportActivityCell-Penetrating Peptide Mediates Intracellular Membrane Passage of Human Papillomavirus L2 Protein to Trigger Retrograde Trafficking
Zhang P, da Silva G, Deatherage C, Burd C, DiMaio D. Cell-Penetrating Peptide Mediates Intracellular Membrane Passage of Human Papillomavirus L2 Protein to Trigger Retrograde Trafficking. Cell 2018, 174: 1465-1476.e13. PMID: 30122350, PMCID: PMC6128760, DOI: 10.1016/j.cell.2018.07.031.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCapsid ProteinsCell-Penetrating PeptidesEndosomesGolgi ApparatusGreen Fluorescent ProteinsHEK293 CellsHeLa CellsHuman papillomavirus 16HumansMutagenesisOncogene Proteins, ViralProtein TransportRecombinant Fusion ProteinsSequence AlignmentVirus AttachmentVirus InternalizationConceptsCell-penetrating peptidesTrans-Golgi networkNormal cell physiologyL2 proteinRetrograde transport pathwayShort protein segmentsHPV L2 proteinTrafficking factorsRetrograde traffickingCationic cell-penetrating peptidesCell physiologyEndosomal membranesProtein segmentsC-terminusBiological roleNon-enveloped virusesRetrograde pathwayL2 capsid proteinsMembrane passageCell penetrating peptideCapsid proteinViral proteinsProteinRetromerTransport pathwaysLipid trafficking by yeast Snx4 family SNX-BAR proteins promotes autophagy and vacuole membrane fusion
Ma M, Kumar S, Purushothaman L, Babst M, Ungermann C, Chi RJ, Burd CG. Lipid trafficking by yeast Snx4 family SNX-BAR proteins promotes autophagy and vacuole membrane fusion. Molecular Biology Of The Cell 2018, 29: 2190-2200. PMID: 29949447, PMCID: PMC6249802, DOI: 10.1091/mbc.e17-12-0743.Peer-Reviewed Original ResearchConceptsVacuole fusionAutophagy pathwaySNX-BAR proteinsCore autophagy machinerySelective autophagy pathwaysHomotypic vacuole fusionVacuole membrane fusionPhosphatidylserine-containing membranesRetrograde transport carriersTurnover of macromoleculesAutophagy intermediatesGlycerophospholipid homeostasisNonselective autophagyBAR domainEndosomal sortingAutophagy machineryFusion competenceLipid traffickingPhosphatidylserine-rich membranesCoat proteinMembrane fusionVacuole membranePhosphatidylethanolamine biosynthesisYeast cellsPhosphatidylethanolamine synthesis
2016
Sphingomyelin is sorted at the trans Golgi network into a distinct class of secretory vesicle
Deng Y, Rivera-Molina FE, Toomre DK, Burd CG. Sphingomyelin is sorted at the trans Golgi network into a distinct class of secretory vesicle. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: 6677-6682. PMID: 27247384, PMCID: PMC4914164, DOI: 10.1073/pnas.1602875113.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSynthesis of sphingomyelinGolgi networkSecretory vesiclesPlasma membraneQuantitative live-cell imagingVesicular transport carriersSorting of proteinsGlycophosphatidylinositol-anchored proteinsPore-forming toxinsLive-cell imagingInterorganelle traffickingAbundant sphingolipidIntracellular traffickingSecretory proteinsSM transportTransport carriersProteinCell imagingTraffickingDistinct classesSpecific carrierVesiclesPrincipal functionSorting
2011
Roles of lipids in Golgi sorting
Burd C, Wood C, Hung C. Roles of lipids in Golgi sorting. The FASEB Journal 2011, 25: 83.2-83.2. DOI: 10.1096/fasebj.25.1_supplement.83.2.Peer-Reviewed Original ResearchGolgi compartmentPI4KGenetic interaction studiesGolgi membrane proteinLocation of enzymesMedial Golgi compartmentActivation of mTORRole of lipidsBiosynthetic cargoMembrane proteinsSorting reactionActing enzymesGolgi stacksKey regulatorOncogenic transformationGolgi apparatusGolgiVps74Trans compartmentEnzymeCompartmentsInteraction studiesProper identityCarbohydrate moietyOrthologs
2004
Functions of Rab GTPases in organelle biogenesis
Burd C, Collins R. Functions of Rab GTPases in organelle biogenesis. Topics In Current Genetics 2004, 10: 65-88. DOI: 10.1007/b97781.Peer-Reviewed Original ResearchRab GTPasesSpecific effector proteinsVesicle tetheringOrganelle biogenesisMembrane traffickingCargo sortingEffector proteinsEndocytic organellesGTP bindingOrganelle transportGTPasesConformational changesBiogenesisDiverse aspectsRabRecent progressOrganellesTraffickingProteinTetheringBindingSortingFusion
2001
FYVE Domain Targets Pib1p Ubiquitin Ligase to Endosome and Vacuolar Membranes*
Shin M, Ogburn K, Varban O, Gilbert P, Burd C. FYVE Domain Targets Pib1p Ubiquitin Ligase to Endosome and Vacuolar Membranes*. Journal Of Biological Chemistry 2001, 276: 41388-41393. PMID: 11526110, DOI: 10.1074/jbc.m105665200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAmino Acid SequenceEndosomesGreen Fluorescent ProteinsIntracellular MembranesLigasesLuminescent ProteinsMolecular Sequence DataPhosphatidylinositol 3-KinasesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidSignal TransductionUbiquitin-Protein Ligase ComplexesUbiquitin-Protein LigasesVacuolesConceptsPI3K productsVacuolar membraneFYVE domainUbiquitin ligaseK productRING-type ubiquitin ligaseUbiquitin ligase activityFYVE fingerFinger motifEukaryotic cellsYeast SaccharomycesCellular proteinsRING domainLigase activityDeletional analysisFusion proteinStructural domainsHistidine residuesPrimary structurePI3KFluorescence microscopyProteinLigaseBindsMembrane
2000
GDP Dissociation Inhibitor Domain II Required for Rab GTPase Recycling*
Gilbert P, Burd C. GDP Dissociation Inhibitor Domain II Required for Rab GTPase Recycling*. Journal Of Biological Chemistry 2000, 276: 8014-8020. PMID: 11116150, DOI: 10.1074/jbc.m008845200.Peer-Reviewed Original ResearchConceptsGDP dissociation inhibitorDomain IIRab GDP dissociation inhibitorRab bindingRab localizationGenetic screenRab GTPasesTransport intermediatesDissociation inhibitorDeletion strainAcceptor membranesCytosolic poolVps21pVesicle fusionRabDomain IMutantsGdi1pYpt1pGeneral defectOrganellesCytosolMembraneDistinct subsetsSec4pDeterminants of NPC1 Expression and Action: Key Promoter Regions, Posttranscriptional Control, and the Importance of a “Cysteine-Rich” Loop
Watari H, Blanchette-Mackie E, Dwyer N, Watari M, Burd C, Patel S, Pentchev P, Strauss J. Determinants of NPC1 Expression and Action: Key Promoter Regions, Posttranscriptional Control, and the Importance of a “Cysteine-Rich” Loop. Experimental Cell Research 2000, 259: 247-256. PMID: 10942596, DOI: 10.1006/excr.2000.4976.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarcinoma, HepatocellularCarrier ProteinsCHO CellsCricetinaeCysteineGene Expression RegulationHumansIntracellular Signaling Peptides and ProteinsLysosomesMembrane GlycoproteinsNiemann-Pick C1 ProteinNiemann-Pick DiseasesPlasmidsProgesteronePromoter Regions, GeneticProtein Structure, TertiaryProteinsRNA Processing, Post-TranscriptionalRNA, MessengerTransfectionTumor Cells, CulturedZincConceptsNiemann-Pick type C diseaseCholesterol trafficking defectType C diseaseC diseaseNPC1 expressionTrafficking defectsNPC1 genePromoter activityMajor transcription initiation siteBase pairsLate endosomal compartmentsTranscription initiation siteTranscription start siteI1061T mutationKey promoter regionChinese hamster cell lineProgesterone-treated cellsHamster cell linesNPC1 mRNA levelsProgesterone-induced increaseZinc-binding activityProtein synthesis inhibitorCT60 cellsThreonine residuesPosttranscriptional regulationVisualizing protein dynamics in yeast with green fluorescent protein
Burd C. Visualizing protein dynamics in yeast with green fluorescent protein. Methods In Enzymology 2000, 327: 61-69. PMID: 11044974, DOI: 10.1016/s0076-6879(00)27267-4.Peer-Reviewed Original ResearchConceptsGreen fluorescent proteinProtein dynamicsUse of GFPFluorescent proteinYeast cell biologyProtein sortingRNA localizationIndividual cellular componentsChromosomal dynamicsProtein localizationKinase signalingCell biologyMutant strainMolecular tagsCellular componentsYeastProteinNucleocytoplasmicBroad arrayLocalizationSignalingBiologySortingMajor impactTags
1999
Phosphatidylinositol 3-Phosphate Recognition by the FYVE Domain
Kutateladze T, Ogburn K, Watson W, de Beer T, Emr S, Burd C, Overduin M. Phosphatidylinositol 3-Phosphate Recognition by the FYVE Domain. Molecular Cell 1999, 3: 805-811. PMID: 10394369, DOI: 10.1016/s1097-2765(01)80013-7.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBinding SitesConserved SequenceDimerizationHumansLiposomesMembrane ProteinsMolecular Sequence DataMolecular WeightMutationNuclear Magnetic Resonance, BiomolecularPhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesPhosphatidylinositolsProtein BindingProtein FoldingProtein Structure, SecondarySolubilitySubstrate SpecificityVesicular Transport ProteinsZincZinc FingersConceptsFYVE domainEndosome autoantigen 1Membrane trafficking eventsHeteronuclear magnetic resonance spectroscopyZinc-binding motifSpecific amino acidsTrafficking eventsEndosome fusionCellular signalingAlpha-helixBeta hairpinAmino acidsPhosphoinositidePhosphatidylinositolMotifStructural featuresRing fingerDomainPtdlnsSignalingProteinHairpinHelixBroad rangeLipidsMolecular Dissection of Guanine Nucleotide Dissociation Inhibitor Function in Vivo Rab-INDEPENDENT BINDING TO MEMBRANES AND ROLE OF RAB RECYCLING FACTORS*
Luan P, Balch W, Emr S, Burd C. Molecular Dissection of Guanine Nucleotide Dissociation Inhibitor Function in Vivo Rab-INDEPENDENT BINDING TO MEMBRANES AND ROLE OF RAB RECYCLING FACTORS*. Journal Of Biological Chemistry 1999, 274: 14806-14817. PMID: 10329679, DOI: 10.1074/jbc.274.21.14806.Peer-Reviewed Original ResearchConceptsRecycling factorNucleotide Dissociation InhibitorFusion of vesiclesSite-directed mutagenesisAmino acid residuesRab deliveryDistinct RabsRab GTPasesRab proteinsRab-GDPDissociation inhibitorMembrane associationEssential proteinsMolecular dissectionEndocytic pathwayGDI functionAcid residuesRabCellular membranesDominant inhibitionMultiple effectorsRate of recyclingInhibitor functionProteinEndogenous poolVac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome
Peterson M, Burd C, Emr S. Vac1p coordinates Rab and phosphatidylinositol 3-kinase signaling in Vps45p-dependent vesicle docking/fusion at the endosome. Current Biology 1999, 9: 159-s1. PMID: 10021387, DOI: 10.1016/s0960-9822(99)80071-2.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingCytoskeletal ProteinsEndosomesFungal ProteinsGolgi ApparatusGTP PhosphohydrolasesGTP-Binding ProteinsGuanine Nucleotide Dissociation InhibitorsGuanosine TriphosphateMacromolecular SubstancesMembrane ProteinsPhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesProtein BindingQa-SNARE ProteinsRab GTP-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsVesicular Transport ProteinsConceptsVacuolar protein sorting (VPS) pathwayVesicle docking/fusionProtein sorting pathwaysDocking/fusionVacuolar protein precursorsMultivalent adaptor proteinLysosome-like vacuolePrevacuolar endosomeVPS genesVps34 phosphatidylinositolLate GolgiGTPase signalsRab GTPaseSorting pathwaysVesicle dockingEndosome transportVacuolar proteinAdaptor proteinEndosomal compartmentsVac1pProtein precursorGolgiTransport stepsVps21pVps45p
1998
Novel pathways, membrane coats and PI kinase regulation in yeast lysosomal trafficking
Burd C, Babst M, Emr S. Novel pathways, membrane coats and PI kinase regulation in yeast lysosomal trafficking. Seminars In Cell And Developmental Biology 1998, 9: 527-533. PMID: 9835640, DOI: 10.1006/scdb.1998.0255.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Protein Complex alpha SubunitsAdaptor Proteins, Vesicular TransportAlkaline PhosphataseBiological TransportCarboxypeptidasesCarrier ProteinsCathepsin ACell MembraneCoated Pits, Cell-MembraneFungal ProteinsIntracellular MembranesLysosomesMembrane ProteinsModels, BiologicalMonomeric Clathrin Assembly ProteinsPhosphatidylinositol 3-KinasesSaccharomyces cerevisiaeVacuolesConceptsSorting pathwaysVesicle docking/fusionAdaptor protein-3 complexProtein sorting signalsDocking/fusionPhosphorylation-dependent ubiquitinationVesicle coat proteinsYeast Saccharomyces cerevisiaeMembrane fusion reactionGolgi recyclingEndocytic cargoFYVE domainSorting signalsKinase regulationEukaryotic cellsImportant mechanistic insightsMembrane transport reactionsRecycling pathwaySaccharomyces cerevisiaeBiosynthetic pathwayCoat proteinRecognition motifMembrane coatMolecular mechanismsLysosomal traffickingAcidic Di-leucine Motif Essential for AP-3–dependent Sorting and Restriction of the Functional Specificity of the Vam3p Vacuolar t-SNARE
Darsow T, Burd C, Emr S. Acidic Di-leucine Motif Essential for AP-3–dependent Sorting and Restriction of the Functional Specificity of the Vam3p Vacuolar t-SNARE. Journal Of Cell Biology 1998, 142: 913-922. PMID: 9722605, PMCID: PMC2132875, DOI: 10.1083/jcb.142.4.913.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Vesicular TransportAlkaline PhosphataseBiological TransportCarboxypeptidasesCathepsin AEndosomesFungal ProteinsLeucineMembrane ProteinsMonomeric Clathrin Assembly ProteinsMutationNerve Tissue ProteinsPhosphoproteinsQa-SNARE ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSNARE ProteinsVesicle-Associated Membrane Protein 3Vesicular Transport ProteinsConceptsPre-vacuolar endosomesVacuolar proteinCarboxypeptidase Y pathwayDi-leucine sequenceOrganelle-specific functionsT-SNARE proteinsAP-3 functionAlkaline phosphatase pathwayYeast Saccharomyces cerevisiaeTransport of proteinsSNAP receptor (SNARE) proteinsT-SNAREsGenetic screenCPY pathwayLeucine signalDelivery of cargoPhosphatase pathwaysRegulated transportMammalian systemsPep12pVam3pSaccharomyces cerevisiaeAccessory proteinsAcid sequenceALP pathway