2022
Actuating tension-loaded DNA clamps drives membrane tubulation
Liu L, Xiong Q, Xie C, Pincet F, Lin C. Actuating tension-loaded DNA clamps drives membrane tubulation. Science Advances 2022, 8: eadd1830. PMID: 36223466, PMCID: PMC9555772, DOI: 10.1126/sciadv.add1830.Peer-Reviewed Original ResearchConceptsDNA clampMembrane tubulationMembrane dynamicsMembrane-remodeling eventsVesicle tubulationConformational changesSpatiotemporal controlDNA signalsCell membraneDNA nanostructuresTubulationMembrane deformationClosed stateOpen stateSelf-assembled DNA nanostructuresOrganismsProteinMembrane tubeArtificial systemsTube widthMembraneDynamics
2019
Engineering Lipid Membranes with Programmable DNA Nanostructures
Shen Q, Grome MW, Yang Y, Lin C. Engineering Lipid Membranes with Programmable DNA Nanostructures. Advanced Biology 2019, 4 PMID: 31934608, PMCID: PMC6957268, DOI: 10.1002/adbi.201900215.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDNA nanostructuresProgrammable DNA nanostructuresGenetic informationLipid membranesCell's genetic informationAmphipathic lipid moleculesLipid/DNA complexesSelf-assembling capabilitiesDNA-based toolsQuantitative biophysical studiesNanoscopic precisionProgrammable nanostructuresControllable structureHybrid materialsChromatin structureGene therapyNanostructuresDistant moleculesSynthetic biologyAbundant biomoleculesLipid moleculesBiophysical studiesDNA complexesExcellent materialCell nucleiStiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation
Grome MW, Zhang Z, Lin C. Stiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation. ACS Applied Materials & Interfaces 2019, 11: 22987-22992. PMID: 31252462, PMCID: PMC6613048, DOI: 10.1021/acsami.9b05401.Peer-Reviewed Original ResearchConceptsVesicle tubulationMembrane-deforming proteinsDNA-based constructsMembrane anchorArtificial assemblageMembrane bindingSubcellular membranesDNA nanostructuresMembrane affinityTubulationLipid tubulesMembraneDNA nanotechnologyTunable architecturesPeptide densitySurface of liposomesProteinNanostructuresAssemblagesBindingAffinity
2012
Purification of DNA-origami nanostructures by rate-zonal centrifugation
Lin C, Perrault SD, Kwak M, Graf F, Shih WM. Purification of DNA-origami nanostructures by rate-zonal centrifugation. Nucleic Acids Research 2012, 41: e40-e40. PMID: 23155067, PMCID: PMC3553994, DOI: 10.1093/nar/gks1070.Peer-Reviewed Original ResearchConceptsDNA origami nanostructuresDNA nanostructuresDNA origami structuresAgarose gel electrophoresisDNA origami constructsNanostructuresAqueous solutionFraction collectionSeparation resolutionGradient mixingPurification processHigh yieldsPurification approachConcentration stepRate zonal centrifugationCentrifuge tubeAgarose gelDyeAvailable equipmentDesaltingGelContaminantsPurificationPreparationSeparation
2011
Synthesis and Characterization of Self-Assembled DNA Nanostructures
Lin C, Ke Y, Chhabra R, Sharma J, Liu Y, Yan H. Synthesis and Characterization of Self-Assembled DNA Nanostructures. Methods In Molecular Biology 2011, 749: 1-11. PMID: 21674361, DOI: 10.1007/978-1-61779-142-0_1.ChaptersConceptsDNA nanostructuresDNA nanotechnologySelf-assembled DNA nanostructuresSuch nanoscale objectsStructural DNA nanotechnologyCharacterization of SelfDNA-based nanodevicesExcellent yieldsNanoscale objectsNanostructuresArtificial nanostructuresMolecular speciesBuilding blocksNanotechnologyFast evolvementMain building blocksNanoarchitecturesLarge varietySynthesisMaterialsNanodevicesPreparationCharacterizationBuilding materialsYield
2009
A Replicable Tetrahedral Nanostructure Self-Assembled from a Single DNA Strand
Li Z, Wei B, Nangreave J, Lin C, Liu Y, Mi Y, Yan H. A Replicable Tetrahedral Nanostructure Self-Assembled from a Single DNA Strand. Journal Of The American Chemical Society 2009, 131: 13093-13098. PMID: 19737020, PMCID: PMC3083857, DOI: 10.1021/ja903768f.Peer-Reviewed Original ResearchConceptsAtomic force microscopy imagingForce microscopy imagingRolling-circle amplificationDNA nanostructuresSingle DNA strandsFerguson analysisMicroscopy imagingTetrahedraStandard molecular cloning techniquesDNA strandsSingle strandsRestriction enzyme digestionCloning techniquesMolecular cloning techniquesDesigner DNA Nanoarchitectures
Lin C, Liu Y, Yan H. Designer DNA Nanoarchitectures. Biochemistry 2009, 48: 1663-1674. PMID: 19199428, PMCID: PMC2765550, DOI: 10.1021/bi802324w.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsStructural DNA nanotechnologyDNA nanotechnologyDNA nanoarchitecturesSelf-assembled architecturesWatson-Crick base pairing rulesProtein-protein interactionsBase pairing rulesDNA nanostructuresMulticomponent nanomachinesPrinciples of DNAArtificial nanostructuresNanometer precisionHelical DNA structureUnicellular diatomsFunctional groupsDNA structureNanotechnologyNanostructuresMolecular speciesNanoarchitecturesSuch eleganceExciting progressHigh yieldsBiological systemsDNA
2008
Mirror Image DNA Nanostructures for Chiral Supramolecular Assemblies
Lin C, Ke Y, Li Z, Wang JH, Liu Y, Yan H. Mirror Image DNA Nanostructures for Chiral Supramolecular Assemblies. Nano Letters 2008, 9: 433-436. PMID: 19063615, PMCID: PMC2669104, DOI: 10.1021/nl803328v.Peer-Reviewed Original ResearchConceptsChiral supramolecular assembliesStructural DNA nanotechnologyNatural d-DNACircular dichroism spectraSupramolecular assembliesDNA supramoleculesAtomic force microscopeDNA nanostructuresDNA nanotechnologyL-DNADichroism spectraVivo medical applicationsNuclease resistanceForce microscopePolyacrylamide gel electrophoresisOpposite chiralityNanostructuresD-DNAPeriodic nanostructuresMedical applicationsSupramoleculesGel electrophoresisSubstantial complementChiralityNanotechnologyIn vivo cloning of artificial DNA nanostructures
Lin C, Rinker S, Wang X, Liu Y, Seeman NC, Yan H. In vivo cloning of artificial DNA nanostructures. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 17626-17631. PMID: 18927233, PMCID: PMC2584761, DOI: 10.1073/pnas.0805416105.Peer-Reviewed Original ResearchConceptsArtificial DNA nanostructuresDNA nanostructuresDNA nanotechnologyGenetic information carriersStandard molecular biology techniquesNanostructuresVersatile methodFour-arm junctionsVivo cloningMolecular biology techniquesNumber of assaysComplex secondary structureBiology techniquesNanotechnologyHigh copy numberLiving cellsFerguson analysisHelper phageCorrect replicationPossible solutionsSecondary structureCopy numberPhagemidCarriersDNA
2006
DNA Tile Based Self‐Assembly: Building Complex Nanoarchitectures
Lin C, Liu Y, Rinker S, Yan H. DNA Tile Based Self‐Assembly: Building Complex Nanoarchitectures. ChemPhysChem 2006, 7: 1641-1647. PMID: 16832805, DOI: 10.1002/cphc.200600260.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDNA tilesSelf-assembled DNA nanostructuresNanometer-scale materialsNanometer-scale componentsComplex nanoarchitecturesDNA nanostructuresNanophotonic devicesAttractive routeExcellent scaffoldProgrammable patternsNanoarchitecturesPotential applicationsExciting progressNanoarraysNanoparticlesNanostructuresRecent developmentsScaffoldsDevicesRouteMaterials
2005
Aptamer‐Directed Self‐Assembly of Protein Arrays on a DNA Nanostructure
Liu Y, Lin C, Li H, Yan H. Aptamer‐Directed Self‐Assembly of Protein Arrays on a DNA Nanostructure. Angewandte Chemie International Edition 2005, 44: 4333-4338. PMID: 15945116, DOI: 10.1002/anie.200501089.Peer-Reviewed Original Research