2019
Effect of overwintering on survival and vector competence of the West Nile virus vector Culex pipiens
Koenraadt CJM, Möhlmann TWR, Verhulst NO, Spitzen J, Vogels CBF. Effect of overwintering on survival and vector competence of the West Nile virus vector Culex pipiens. Parasites & Vectors 2019, 12: 147. PMID: 30917854, PMCID: PMC6437999, DOI: 10.1186/s13071-019-3400-4.Peer-Reviewed Original ResearchConceptsVector competencePipiens mosquitoesBiotype pipiensMosquito-borne virusLaboratory-reared CxReal-time PCROlder ageConclusionsThis studyCulex pipiens mosquitoesBiotype compositionLaboratory-reared mosquitoesNile virusLaboratory-reared femalesSurvivalMonthsPipiens biotypesTwo-thirdsCulex pipiensWNV transmissionWest Nile virus vector Culex pipiensBiotype molestusPipiens femalesWNVMosquitoesSpecies Cx
2016
Latitudinal Diversity of Culex pipiens Biotypes and Hybrids in Farm, Peri-Urban, and Wetland Habitats in Europe
Vogels CB, Möhlmann TW, Melsen D, Favia G, Wennergren U, Koenraadt CJ. Latitudinal Diversity of Culex pipiens Biotypes and Hybrids in Farm, Peri-Urban, and Wetland Habitats in Europe. PLOS ONE 2016, 11: e0166959. PMID: 27870890, PMCID: PMC5117740, DOI: 10.1371/journal.pone.0166959.Peer-Reviewed Original ResearchConceptsHabitat typesPipiens mosquitoesPipiens biotypesPopulation dynamics studiesMosquito Magnet Liberty Plus trapsWest Nile virusCulex pipiens mosquitoesLatitudinal diversityWetland habitatsCulex pipiens biotypesReal-time PCRRelative abundanceBiotype levelSpecies CxBiotypesSurveillance trapsPipiensMolestusWNV outbreaksHybridsNoncoding Subgenomic Flavivirus RNA Is Processed by the Mosquito RNA Interference Machinery and Determines West Nile Virus Transmission by Culex pipiens Mosquitoes
Göertz GP, Fros JJ, Miesen P, Vogels CB, van der Bent ML, Geertsema C, Koenraadt CJ, van Rij RP, van Oers MM, Pijlman GP. Noncoding Subgenomic Flavivirus RNA Is Processed by the Mosquito RNA Interference Machinery and Determines West Nile Virus Transmission by Culex pipiens Mosquitoes. Journal Of Virology 2016, 90: 10145-10159. PMID: 27581979, PMCID: PMC5105652, DOI: 10.1128/jvi.00930-16.Peer-Reviewed Original ResearchConceptsSubgenomic flavivirus RNARNA interference machineryCulex pipiens mosquitoesWest Nile virusInterference machinerySfRNA productionPipiens mosquitoesBiological functionsUntranslated regionNile virusPivotal biological functionsIntrathoracic injectionWild-type WNVRNA deep sequencingKey biological functionsBlood mealFlavivirus RNAMosquito cell linesFlavivirus West Nile virusViral genomic RNAMosquito midgut barrierMutant West Nile virusTick-borne flavivirusesWNV-infected mosquitoesMammalian cells
2015
Comparative Usutu and West Nile virus transmission potential by local Culex pipiens mosquitoes in north-western Europe
Fros JJ, Miesen P, Vogels CB, Gaibani P, Sambri V, Martina BE, Koenraadt CJ, van Rij RP, Vlak JM, Takken W, Pijlman GP. Comparative Usutu and West Nile virus transmission potential by local Culex pipiens mosquitoes in north-western Europe. One Health 2015, 1: 31-36. PMID: 28616462, PMCID: PMC5441354, DOI: 10.1016/j.onehlt.2015.08.002.Peer-Reviewed Original ResearchWest Nile virusUsutu virusMosquito-borne flavivirusHigh mortality rateVirus transmission potentialNeuroinvasive diseaseUSUV transmissionWNV infectionEuropean mosquitoesMortality rateInfected mosquitoesInfection rateMosquito midgutSmall interferingNile virusTransmission potentialVirusEntire viral genomeTransmission dynamicsVector competenceCulex pipiens mosquitoesRNA deep sequencingMosquitoesViral genomeSmall RNA deep sequencingWest Nile Virus: High Transmission Rate in North-Western European Mosquitoes Indicates Its Epidemic Potential and Warrants Increased Surveillance
Fros JJ, Geertsema C, Vogels CB, Roosjen PP, Failloux AB, Vlak JM, Koenraadt CJ, Takken W, Pijlman GP. West Nile Virus: High Transmission Rate in North-Western European Mosquitoes Indicates Its Epidemic Potential and Warrants Increased Surveillance. PLOS Neglected Tropical Diseases 2015, 9: e0003956. PMID: 26225555, PMCID: PMC4520649, DOI: 10.1371/journal.pntd.0003956.Peer-Reviewed Original ResearchConceptsWest Nile virusEpidemic potentialLineage 1 West Nile VirusInfectious blood mealFurther epidemic spreadLineage 2 West Nile virusNeuroinvasive diseaseIntrathoracic injectionEuropean mosquitoesPathogenic flavivirusesInfection rateVirus activityLarge outbreakVirus disseminationWNV infection ratesNile virusWNV activityWNV lineagesBlood mealVector competenceInfectionCulex pipiens mosquitoesWNV transmissionEpidemic spreadDisease-free regions