2017
Polymerization of hexamethylene diisocyanate in solution and a 260.23 m/z [M+H]+ ion in exposed human cells
Wisnewski AV, Liu J, Redlich CA, Nassar AF. Polymerization of hexamethylene diisocyanate in solution and a 260.23 m/z [M+H]+ ion in exposed human cells. Analytical Biochemistry 2017, 543: 21-29. PMID: 29175138, PMCID: PMC5826792, DOI: 10.1016/j.ab.2017.11.017.Peer-Reviewed Original ResearchConceptsHexamethylene diisocyanateCollision-induced dissociation (CID) fragmentation patternsNucleophilic additionImportant industrial chemicalPrimary aminesFragmentation patternsO groupLC-MS/MSLC-MS/MS analysisLC-MSReaction productsIonsMS analysisFormula CIndustrial chemicalsDiisocyanatePolymerizationPolymersReaction targetWaterSolutionAminesDefinitive characterizationMoleculesDimers
2010
Antigenic changes in human albumin caused by reactivity with the occupational allergen diphenylmethane diisocyanate
Wisnewski AV, Liu J, Redlich CA. Antigenic changes in human albumin caused by reactivity with the occupational allergen diphenylmethane diisocyanate. Analytical Biochemistry 2010, 400: 251-258. PMID: 20123080, PMCID: PMC3106273, DOI: 10.1016/j.ab.2010.01.037.Peer-Reviewed Original ResearchConceptsHigh-performance liquid chromatographyDiphenylmethane diisocyanateConjugation sitesReaction productsCross-linking agentTandem mass spectrometryPolyurethane productionMass spectrometryMDI-exposed workersCause of asthmaHuman albuminSerum immunoglobulin GLiquid chromatographyReaction specificityDiisocyanateSecond lysineMDI conjugationAirway inflammationAnalytical methodSelf proteinsProteomic methodologiesEpitope specificityAntigenic changesClinical researchConjugates
2002
Diisocyanate asthma: clinical aspects and immunopathogenesis
Redlich CA, Karol MH. Diisocyanate asthma: clinical aspects and immunopathogenesis. International Immunopharmacology 2002, 2: 213-224. PMID: 11811926, DOI: 10.1016/s1567-5769(01)00174-6.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDiisocyanate asthmaProduction of polyurethanesChemical reactivityBiological nucleophilesClinical aspectsChemical interactionReactive chemicalsRoute of exposureDiisocyanateSimple diagnostic testOccupational asthmaEarly diseaseRespiratory tractNonimmunological mechanismsWorkplace exposuresAsthmaUseful markerReactionDiagnostic testsDiseasePathogenesisImmunopathogenesisBiomoleculesNucleophilesExposure
2001
Development of immunoassays for biomonitoring of hexamethylene diisocyanate exposure.
Lemus R, Lukinskeine L, Bier ME, Wisnewski AV, Redlich CA, Karol MH. Development of immunoassays for biomonitoring of hexamethylene diisocyanate exposure. Environmental Health Perspectives 2001, 109: 1103-1108. PMID: 11712993, PMCID: PMC1240469, DOI: 10.1289/ehp.011091103.Peer-Reviewed Original ResearchConceptsHexamethylene diisocyanateHuman serum albuminLaser desorption timeMatrix-assisted laser desorption timeFlight mass spectrometryWorkplace air monitoringMass spectrometryDesorption timeDiisocyanateAdductsDevelopment of immunoassaysHSA antigenSerum albuminMicrotiter platesReactionAir monitoringHexamethylene diisocyanate exposurePpbAbsence of reactionSensitive bioassayNumerous samplesSpectrometryDiphenylPolyurethaneCoatings