2014
Pivoting between Calmodulin Lobes Triggered by Calcium in the Kv7.2/Calmodulin Complex
Alaimo A, Alberdi A, Gomis-Perez C, Fernández-Orth J, Bernardo-Seisdedos G, Malo C, Millet O, Areso P, Villarroel A. Pivoting between Calmodulin Lobes Triggered by Calcium in the Kv7.2/Calmodulin Complex. PLOS ONE 2014, 9: e86711. PMID: 24489773, PMCID: PMC3904923, DOI: 10.1371/journal.pone.0086711.Peer-Reviewed Original ResearchConceptsC-lobeC-terminal segmentPrincipal molecular componentsAssociation of CaMChannel traffickingKv7.2 channelsMolecular mechanismsMolecular eventsEndoplasmic reticulumN-lobeMolecular componentsHelix BCalmodulin complexHelix A.CalmodulinData highlightKv7.2Calmodulin lobesM channelsComplementary approachesNeuronal excitabilityFluorometric assayTraffickingReporterReticulum
2012
Surface Expression and Subunit Specific Control of Steady Protein Levels by the Kv7.2 Helix A-B Linker
Aivar P, Fernández-Orth J, Gomis-Perez C, Alberdi A, Alaimo A, Rodríguez MS, Giraldez T, Miranda P, Areso P, Villarroel A. Surface Expression and Subunit Specific Control of Steady Protein Levels by the Kv7.2 Helix A-B Linker. PLOS ONE 2012, 7: e47263. PMID: 23115641, PMCID: PMC3480381, DOI: 10.1371/journal.pone.0047263.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsHEK293 CellsHumansKCNQ2 Potassium ChannelMicroscopy, ConfocalMolecular Sequence DataPatch-Clamp TechniquesXenopusConceptsB linkerPlasma membraneHelix ATetrameric channel assemblyIntracellular C-terminusAmino acid sequenceSteady-state amountsSurface expressionSteady-state levelsProtein degradationAcid sequenceC-terminusChannel assemblyFunctional channelsIntracellular distributionProtein levelsProteinSpecific controlKv7.2Detectable impactM-currentExpressionNeuronal excitabilityMembraneIntrinsic signals