2017
Determining Phosphodiesterase Activity (Radioactive Assay)
Kazmierczak BI. Determining Phosphodiesterase Activity (Radioactive Assay). Methods In Molecular Biology 2017, 1657: 279-283. PMID: 28889301, DOI: 10.1007/978-1-4939-7240-1_21.Peer-Reviewed Original ResearchSynthesis of [32P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinations
Kazmierczak BI. Synthesis of [32P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinations. Methods In Molecular Biology 2017, 1657: 23-29. PMID: 28889283, DOI: 10.1007/978-1-4939-7240-1_3.Peer-Reviewed Original ResearchDetermining Diguanylate Cyclase Activity (Radioactive Assay)
Kazmierczak BI. Determining Diguanylate Cyclase Activity (Radioactive Assay). Methods In Molecular Biology 2017, 1657: 285-289. PMID: 28889302, DOI: 10.1007/978-1-4939-7240-1_22.Peer-Reviewed Original Research
2007
Immune recognition of Pseudomonas aeruginosa mediated by the IPAF/NLRC4 inflammasome
Sutterwala FS, Mijares LA, Li L, Ogura Y, Kazmierczak BI, Flavell RA. Immune recognition of Pseudomonas aeruginosa mediated by the IPAF/NLRC4 inflammasome. Journal Of Experimental Medicine 2007, 204: 3235-3245. PMID: 18070936, PMCID: PMC2150987, DOI: 10.1084/jem.20071239.Peer-Reviewed Original ResearchConceptsInnate immune responseImmune responseP. aeruginosaCaspase-1Proinflammatory cytokine productionProinflammatory cytokines interleukinInfection of macrophagesCell deathHost cellsCapase-1Pseudomonas aeruginosaOpportunistic infectionsCytokine productionCytokines interleukinInflammatory responseImmune recognitionNLRC4 inflammasomeEffector moleculesType III secretion systemInfectionIPAFMacrophagesGram-negative bacteriumDeathAeruginosa
2001
Rho GTPase activity modulates Pseudomonas aeruginosa internalization by epithelial cells
Kazmierczak B, Jou T, Mostov K, Engel J. Rho GTPase activity modulates Pseudomonas aeruginosa internalization by epithelial cells. Cellular Microbiology 2001, 3: 85-98. PMID: 11207623, DOI: 10.1046/j.1462-5822.2001.00091.x.Peer-Reviewed Original ResearchConceptsRho-GTP levelsP. aeruginosa internalizationRho familyEpithelial cellsGTP levelsRho-specific inhibitorRho family GTPasesC3 ADP-ribosyltransferaseRho GTPase activityPathogen Pseudomonas aeruginosaGram-negative pathogen Pseudomonas aeruginosaMadin-Darby canine kidney cellsPseudomonas aeruginosa internalizationCanine kidney cellsNative RhoActive alleleBacterial internalizationGTPase activityP. aeruginosa strain PA103Fusion constructsADP-ribosyltransferaseLatrunculin A.Active Rac1Bacterial uptakeHeLa cells