2000
Prostaglandin E2 interaction with AVP: effects on AQP2 phosphorylation and distribution
Zelenina M, Christensen B, Palmér J, Nairn A, Nielsen S, Aperia A. Prostaglandin E2 interaction with AVP: effects on AQP2 phosphorylation and distribution. American Journal Of Physiology. Renal Physiology 2000, 278: f388-f394. PMID: 10710543, DOI: 10.1152/ajprenal.2000.278.3.f388.Peer-Reviewed Original ResearchConceptsTranslocation of AQP2AQP2 phosphorylationPlasma membraneAquaporin-2Subcellular distributionPlasma membrane-enriched fractionVesicle-enriched fractionsMembrane-enriched fractionDuct water permeabilityConsensus sitesIntracellular vesiclesPhosphorylationDifferential centrifugation techniqueAction of arginineRenal inner medullaE2 interactionRat renal inner medullaTranslocationInner medullaDose-dependent mannerWater channelsMembraneDephosphorylationTraffickingProtein
1995
Immunochemical localization of calcium/calmodulin‐dependent protein kinase I
Picciotto M, Zoli M, Bertuzzi G, Nairn A. Immunochemical localization of calcium/calmodulin‐dependent protein kinase I. Synapse 1995, 20: 75-84. PMID: 7624832, DOI: 10.1002/syn.890200111.Peer-Reviewed Original ResearchConceptsKinase IProtein kinase ICaM kinase INon-neuronal tissuesImmunoreactive speciesCalmodulin-dependent protein kinase IGlutathione S-transferase fusion proteinCalcium/calmodulin-dependent protein kinase IRat brainDependent protein kinase ISubcellular fractionation studiesRecombinant kinasesRat brain enzymeNeuronal cell bodiesCytosolic localizationProtein kinaseMultiple immunoreactive speciesMajor immunoreactive speciesFusion proteinMultiple neuronal processesWidespread cellMajor immunoreactive bandRat cDNAPrimary structureSynapsin I.
1994
Subcellular localization of CFTR to endosomes in a ductal epithelium
Webster P, Vanacore L, Nairn A, Marino C. Subcellular localization of CFTR to endosomes in a ductal epithelium. American Journal Of Physiology 1994, 267: c340-c348. PMID: 7521124, DOI: 10.1152/ajpcell.1994.267.2.c340.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorEndocytosisEpitheliumFluorescent Antibody TechniqueImmunohistochemistryMaleMembrane ProteinsMicroscopy, FluorescenceOrganellesRatsRats, Sprague-DawleyReceptors, Cell SurfaceSubcellular FractionsSubmandibular GlandTissue DistributionConceptsCystic fibrosis transmembrane conductance regulatorPlasma membraneFibrosis transmembrane conductance regulatorApical plasma membraneAnti-CFTR antibodiesNormal epithelial cell populationsTransmembrane conductance regulatorCytochemical evidenceReceptor-mediated endocytosisCFTR moleculesEpithelial cell populationsCellular processesSubcellular compartmentsSubcellular localizationEarly endosomesMembrane recyclingConductance regulatorSubcellular distributionSubapical vesiclesApical poleEndosomesCFTR functionImmunoelectron microscopyCell populationsCFTR immunoreactivity
1992
Distribution of Protein Phosphatase Inhibitor‐1 in Brain and Peripheral Tissues of Various Species: Comparison with DARPP‐32
Hemmings H, Girault J, Nairn A, Bertuzzi G, Greengard P. Distribution of Protein Phosphatase Inhibitor‐1 in Brain and Peripheral Tissues of Various Species: Comparison with DARPP‐32. Journal Of Neurochemistry 1992, 59: 1053-1061. PMID: 1353788, DOI: 10.1111/j.1471-4159.1992.tb08347.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCarrier ProteinsCentral Nervous SystemDopamine and cAMP-Regulated Phosphoprotein 32Enzyme InhibitorsFemaleIntracellular Signaling Peptides and ProteinsMaleNerve Tissue ProteinsPhosphoproteinsProteinsRatsRats, Inbred StrainsSubcellular FractionsTissue DistributionTyrosine 3-MonooxygenaseVertebrates
1988
Skeletal muscle sarcolemma proteins as targets for adenosine 3′:5′-monophosphate-dependent and calcium-dependent protein kinases
Walaas S, Horn R, Nairn A, Walaas O, Adler A. Skeletal muscle sarcolemma proteins as targets for adenosine 3′:5′-monophosphate-dependent and calcium-dependent protein kinases. Archives Of Biochemistry And Biophysics 1988, 262: 245-258. PMID: 3355169, DOI: 10.1016/0003-9861(88)90186-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsMicroscopy, ElectronMuscle ProteinsPeptide MappingPhosphoproteinsProtein KinasesRatsSarcolemmaSubcellular FractionsConceptsCalcium-dependent protein kinaseProtein kinaseProtein phosphorylationPhosphorylation systemRat skeletal muscle plasma membranesCGMP-dependent protein kinaseIntrinsic membrane proteinsProtein phosphorylation systemsSkeletal muscle cellsSkeletal muscle plasma membranesSarcolemma proteinsMembrane proteinsProtein speciesMuscle plasma membranePlasma membraneMembrane targetsSpecific substratesKinaseMultiple hormonesDistinct setsProteinPhosphoproteinMuscle cellsPhosphorylationReticulum fractionsPurification and characterization of PCPP‐260: A Purkinje cell‐enriched cyclic amp‐regulated membrane phosphoprotein of Mr 260,000
Weeks G, Picciotto M, Nairn A, Walaas S, Greengard P. Purification and characterization of PCPP‐260: A Purkinje cell‐enriched cyclic amp‐regulated membrane phosphoprotein of Mr 260,000. Synapse 1988, 2: 89-96. PMID: 2844000, DOI: 10.1002/syn.890020112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCyclic AMPMembrane ProteinsMolecular WeightPeptide MappingPhosphoproteinsPhosphorylationPurkinje CellsSubcellular FractionsConceptsCAMP-dependent protein kinaseMembrane proteinsProtein kinaseN-lauryl sarcosineIntegral membrane proteinsMajor tryptic phosphopeptidesPhosphoamino acid analysisTotal membrane proteinSodium dodecyl sulfate-polyacrylamide gel electrophoresisMembrane phosphoproteinDodecyl sulfate-polyacrylamide gel electrophoresisTryptic phosphopeptidesSulfate-polyacrylamide gel electrophoresisPossible functional roleProminent proteinsAlpha-methyl mannosideParticulate fractionMammalian cerebellumFunctional roleProteinPeptide mappingConcanavalin A-agaroseGel electrophoresisAcid analysisA-agarose