1992
Increased phosphorylation of elongation factor 2 in Alzheimer's disease
Johnson G, Gotlib J, Haroutunian V, Bierer L, Nairn A, Merril C, Wallace W. Increased phosphorylation of elongation factor 2 in Alzheimer's disease. Brain Research 1992, 15: 319-326. PMID: 1331687, DOI: 10.1016/0169-328x(92)90124-t.Peer-Reviewed Original ResearchConceptsDisease brainAlzheimer's diseaseAlzheimer's disease brainFactor 2AD homogenatesAD tissueElongation factor 2Brain homogenatesSame brainDiseaseVariant isoformsProtein synthesisPhosphorylated formInhibits protein synthesisBrainUnaffected areasHomogenatesAcidic isoformsPhosphorylationGene expressionEF-2
1989
Insulin rapidly induces the biosynthesis of elongation factor 2
Levenson R, Nairn A, Blackshear P. Insulin rapidly induces the biosynthesis of elongation factor 2. Journal Of Biological Chemistry 1989, 264: 11904-11911. PMID: 2663845, DOI: 10.1016/s0021-9258(18)80152-1.Peer-Reviewed Original ResearchConceptsElongation factor 2EF-2Overall protein synthesisProtein synthesisProtein translation apparatusEukaryotic elongation factor 2Two-dimensional gel electrophoresisHuman insulin receptorNIH 3T3 cellsFactor 2Translation apparatusMature speciesMRNA translationActinomycin D. ThusRNA transcription inhibitorSuch proteinsIndividual proteinsInsulin inductionPreferential expressionPrecursor formInsulin receptorSerum deprivationProteinGel autoradiographsGel electrophoresisThrombin and Histamine Stimulate the Phosphorylation of Elongation Factor 2 in Human Umbilical Vein Endothelial Cells
Mackie K, Nairn A, Hampel G, Lam G, Jaffe E. Thrombin and Histamine Stimulate the Phosphorylation of Elongation Factor 2 in Human Umbilical Vein Endothelial Cells. Journal Of Biological Chemistry 1989, 264: 1748-1753. PMID: 2536373, DOI: 10.1016/s0021-9258(18)94250-x.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsElongation factor 2EF-2Calmodulin-dependent protein kinase IIIUmbilical vein endothelial cellsProtein synthesisVein endothelial cellsProtein kinase IIIPhosphoamino acid analysisFactor 2Kinase IIICell protein synthesisProtein phosphorylationCultured human umbilical vein endothelial cellsEndothelial cellsPeptidyl-tRNAPhosphorylationBiochemical studiesRapid transient increaseAcid analysisPhorbol dibutyrateIncubation of HUVECsIntracellular calcium levelsProteinEffect of thrombin