1998
Actions of Genistein on Cystic Fibrosis Transmembrane Conductance Regulator Channel Gating
Wang F, Zeltwanger S, Yang I, Nairn A, Hwang T. Actions of Genistein on Cystic Fibrosis Transmembrane Conductance Regulator Channel Gating. The Journal Of General Physiology 1998, 111: 477-490. PMID: 9482713, PMCID: PMC2217116, DOI: 10.1085/jgp.111.3.477.Peer-Reviewed Original ResearchConceptsCystic Fibrosis Transmembrane Conductance Regulator Channel GatingCFTR channelsSerine/threonine proteinTyrosine kinaseCystic fibrosis transmembrane conductance regulator (CFTR) channel activityDirect bindingHi-5 insect cellsCFTR channel currentsTyrosine phosphatase inhibitorMicroM genisteinProtein kinase AEffects of genisteinNonhydrolyzable ATP analogRecombinant CFTRProtein phosphatasePossible molecular mechanismsCFTR gatingInsect cellsPhosphatase inhibitorCalyculin ACFTR proteinAbsence of genisteinATP hydrolysisKinase ANIH3T3 cells
1997
Mutation of the Protein Kinase C Phosphorylation Site on Rat α1 Na+,K+-ATPase Alters Regulation of Intracellular Na+ and pH and Influences Cell Shape and Adhesiveness*
Belusa R, Wang Z, Matsubara T, Sahlgren B, Dulubova I, Nairn A, Ruoslahti E, Greengard P, Aperia A. Mutation of the Protein Kinase C Phosphorylation Site on Rat α1 Na+,K+-ATPase Alters Regulation of Intracellular Na+ and pH and Influences Cell Shape and Adhesiveness*. Journal Of Biological Chemistry 1997, 272: 20179-20184. PMID: 9242694, DOI: 10.1074/jbc.272.32.20179.Peer-Reviewed Original ResearchConceptsProtein kinase CProtein kinase APhosphorylation sitesProtein kinase C phosphorylation sitesKinase C phosphorylation sitesC phosphorylation sitesSites of phosphorylationATPase alpha1Influences cell shapePKC phosphorylation sitesEukaryotic cellsATP hydrolysisPKC phosphorylationRat α1COS cellsCell shapeKinase AWild typeSer-23Kinase CCell adhesionFunctional roleAlters regulationUntransfected cellsPhosphorylation
1994
Regulation of CFTR channel gating
Gadsby D, Nairn A. Regulation of CFTR channel gating. Trends In Biochemical Sciences 1994, 19: 513-518. PMID: 7531880, DOI: 10.1016/0968-0004(94)90141-4.Peer-Reviewed Original ResearchConceptsChannel gatingCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelAMP-dependent protein kinaseCFTR channel gatingReceptor-mediated activationRegulatory domainProtein kinaseATP hydrolysisCFTR channelsCl- channelsEpithelial cellsChannel openingComplex mechanismsCellsRecent advancesKinaseGenesPhosphorylationSerineGatingCFTRMutationsRegulationStemActivationRegulation of the gating of cystic fibrosis transmembrane conductance regulator C1 channels by phosphorylation and ATP hydrolysis.
Hwang T, Nagel G, Nairn A, Gadsby D. Regulation of the gating of cystic fibrosis transmembrane conductance regulator C1 channels by phosphorylation and ATP hydrolysis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 4698-4702. PMID: 7515176, PMCID: PMC43855, DOI: 10.1073/pnas.91.11.4698.Peer-Reviewed Original ResearchConceptsCFTR channelsATP hydrolysisPresence of ATPDomains of CFTRCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelProtein kinase AATP analogue 5'Kinase AOpen probabilityAMP-PNPPhosphorylationLow open probabilityCl- channelsATPATP actionIntact cardiac myocytesNucleotidesCFTRSecond siteExcised patchesHigh open probabilityCardiac myocytesChannel closingC1 channelsAnalogue 5Coupling of CFTR Cl− channel gating to an ATP hydrolysis cycle
Baukrowitz T, Hwang T, Nairn A, Gadsby D. Coupling of CFTR Cl− channel gating to an ATP hydrolysis cycle. Neuron 1994, 12: 473-482. PMID: 7512348, DOI: 10.1016/0896-6273(94)90206-2.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorATP hydrolysis cycleHydrolysis cycleCFTR channelsFibrosis transmembrane conductance regulatorProtein kinase ATransmembrane conductance regulatorATP hydrolysisKinase AConductance regulatorNucleoside triphosphatesChannel openingInorganic phosphate analogueATPPhosphate analogueCardiac myocytesInorganic phosphateMean open timeRegulatorHydrolysis productsBeF3Open timeCycleTriphosphateRegulation of CFTR channel gating.
Gadsby D, Hwang T, Baukrowitz T, Nagel G, Horie M, Nairn A. Regulation of CFTR channel gating. The Journal Of Physiological Sciences 1994, 44 Suppl 2: s183-92. PMID: 7752525.Peer-Reviewed Original ResearchConceptsNon-hydrolyzable ATP analog AMP-PNPCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelAMP-PNPCFTR channel gatingProtein kinase A (PKA) phosphorylationATP analogue AMP-PNPAnalogue AMP-PNPCFTR's twoA PhosphorylationATP hydrolysisChannel gatingCl- channelsChannel openingNBDRegulationMultiple sitesPhosphorylationCFTROrthovanadateATPGatingDomain