2009
Semaphorin3a regulates endothelial cell number and podocyte differentiation during glomerular development
Reidy KJ, Villegas G, Teichman J, Veron D, Shen W, Jimenez J, Thomas D, Tufro A. Semaphorin3a regulates endothelial cell number and podocyte differentiation during glomerular development. Development 2009, 136: 3979-3989. PMID: 19906865, PMCID: PMC2778745, DOI: 10.1242/dev.037267.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell CountCell DifferentiationCell NucleusCoculture TechniquesEndothelial CellsFluorescent Antibody Technique, DirectFluorescent DyesGene DeletionGene Expression Regulation, DevelopmentalGriffoniaImmunohistochemistryIn Situ Nick-End LabelingIndolesKidney GlomerulusMiceMice, KnockoutMice, Mutant StrainsMice, TransgenicPlant LectinsPodocytesRatsRecombinant ProteinsSemaphorin-3AConceptsVascular patterningPodocyte differentiationNegative regulatorNormal glomerular filtration barrierGlomerular developmentEssential negative regulatorEndothelial cell survivalFunction mouse modelsTight regulationEndothelial cell apoptosisUreteric budMature podocytesVascular developmentCell survivalCrucial roleGuidance proteinsSlit diaphragmCell apoptosisGlomerular filtration barrierRegulatorFiltration barrierGlomerular endothelial cell apoptosisCell numberEndothelial cellsEndothelial cell number
2007
Semaphorin3a inhibits ureteric bud branching morphogenesis
Tufro A, Teichman J, Woda C, Villegas G. Semaphorin3a inhibits ureteric bud branching morphogenesis. Cells And Development 2007, 125: 558-568. PMID: 18249526, PMCID: PMC3992265, DOI: 10.1016/j.mod.2007.12.003.Peer-Reviewed Original ResearchConceptsUreteric budUreteric bud branchingAkt survival pathwayUreteric bud treeUreteric bud cellsAntisense morpholinoClass 3 semaphorinsVascular patterningMetanephric organ cultureFactor signalingMouse embryosNegative regulatorKidney developmentSurvival pathwaysGuidance proteinsNeurotrophic Factor SignalingSema3A effectBudsRenal epitheliumMorphogenesisBud cellsMutant miceRecombinant Sema3AGrowth factorSema3ACrosstalk between VEGF-A/VEGFR2 and GDNF/RET signaling pathways
Tufro A, Teichman J, Banu N, Villegas G. Crosstalk between VEGF-A/VEGFR2 and GDNF/RET signaling pathways. Biochemical And Biophysical Research Communications 2007, 358: 410-416. PMID: 17490619, DOI: 10.1016/j.bbrc.2007.04.146.Peer-Reviewed Original ResearchConceptsVascular endothelial growth factorCell proliferationEndothelial growth factorNeurotrophic factorGDNF/RETEmbryonic kidney explantsUreteric bud branchingUreteric bud cellsC-RetGrowth factorRT-PCRVEGFNovel cooperative interactionVEGFR2Branching morphogenesisRETVEGFR2 autophosphorylationGDNFKidney developmentBud cellsUreteric budAdditive effectMajor regulatorKidney explantsPhosphorylation