2013
Excess Podocyte Semaphorin-3A Leads to Glomerular Disease Involving PlexinA1–Nephrin Interaction
Reidy KJ, Aggarwal PK, Jimenez JJ, Thomas DB, Veron D, Tufro A. Excess Podocyte Semaphorin-3A Leads to Glomerular Disease Involving PlexinA1–Nephrin Interaction. American Journal Of Pathology 2013, 183: 1156-1168. PMID: 23954273, PMCID: PMC3791681, DOI: 10.1016/j.ajpath.2013.06.022.Peer-Reviewed Original Research
2009
Semaphorin3a regulates endothelial cell number and podocyte differentiation during glomerular development
Reidy KJ, Villegas G, Teichman J, Veron D, Shen W, Jimenez J, Thomas D, Tufro A. Semaphorin3a regulates endothelial cell number and podocyte differentiation during glomerular development. Development 2009, 136: 3979-3989. PMID: 19906865, PMCID: PMC2778745, DOI: 10.1242/dev.037267.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell CountCell DifferentiationCell NucleusCoculture TechniquesEndothelial CellsFluorescent Antibody Technique, DirectFluorescent DyesGene DeletionGene Expression Regulation, DevelopmentalGriffoniaImmunohistochemistryIn Situ Nick-End LabelingIndolesKidney GlomerulusMiceMice, KnockoutMice, Mutant StrainsMice, TransgenicPlant LectinsPodocytesRatsRecombinant ProteinsSemaphorin-3AConceptsVascular patterningPodocyte differentiationNegative regulatorNormal glomerular filtration barrierGlomerular developmentEssential negative regulatorEndothelial cell survivalFunction mouse modelsTight regulationEndothelial cell apoptosisUreteric budMature podocytesVascular developmentCell survivalCrucial roleGuidance proteinsSlit diaphragmCell apoptosisGlomerular filtration barrierRegulatorFiltration barrierGlomerular endothelial cell apoptosisCell numberEndothelial cellsEndothelial cell number
2007
Semaphorin3a inhibits ureteric bud branching morphogenesis
Tufro A, Teichman J, Woda C, Villegas G. Semaphorin3a inhibits ureteric bud branching morphogenesis. Cells And Development 2007, 125: 558-568. PMID: 18249526, PMCID: PMC3992265, DOI: 10.1016/j.mod.2007.12.003.Peer-Reviewed Original ResearchConceptsUreteric budUreteric bud branchingAkt survival pathwayUreteric bud treeUreteric bud cellsAntisense morpholinoClass 3 semaphorinsVascular patterningMetanephric organ cultureFactor signalingMouse embryosNegative regulatorKidney developmentSurvival pathwaysGuidance proteinsNeurotrophic Factor SignalingSema3A effectBudsRenal epitheliumMorphogenesisBud cellsMutant miceRecombinant Sema3AGrowth factorSema3ASemaphorin3a disrupts podocyte foot processes causing acute proteinuria
Tapia R, Guan F, Gershin I, Teichman J, Villegas G, Tufro A. Semaphorin3a disrupts podocyte foot processes causing acute proteinuria. Kidney International 2007, 73: 733-740. PMID: 18075495, DOI: 10.1038/sj.ki.5002726.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCytoskeletal ProteinsDown-RegulationGlomerular Basement MembraneGlomerular Filtration RateIntracellular Signaling Peptides and ProteinsMaleMembrane ProteinsMiceMice, Inbred StrainsPermeabilityPodocytesProteinuriaRecombinant ProteinsSemaphorin-3AVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-1Vascular Endothelial Growth Factor Receptor-2ConceptsPodocyte foot process effacementNephrotic range proteinuriaEndothelial cell damageVascular endothelial growth factorSlit diaphragm protein podocinFoot process effacementEndothelial growth factorReceptor 2 signalingVascular endothelial growth factor receptor 2 signalingAcute proteinuriaRange proteinuriaReceptor expressionVascular endothelial growth factor 165Process effacementBarrier homeostasisAdult mouse kidneyUltrastructural abnormalitiesSemaphorin3AEndothelial cellsCell damageGrowth factorMouse kidneyGuidance proteinsProteinuriaDownregulation
2006
Semaphorin 3C regulates endothelial cell function by increasing integrin activity
Banu N, Teichman J, Dunlap‐Brown M, Villegas G, Tufro A, Banu N, Teichman J, Dunlap‐Brown M, Villegas G, Tufro A. Semaphorin 3C regulates endothelial cell function by increasing integrin activity. The FASEB Journal 2006, 20: 2150-2152. PMID: 16940438, DOI: 10.1096/fj.05-5698fje.Peer-Reviewed Original ResearchConceptsSema 3CEndothelial cell functionDirectional migrationStarvation-induced apoptosisCell functionCapillary-like network formationCardiovascular patterningClass 3 semaphorinsIntegrin phosphorylationSerine phosphorylationCollagen I gelsVascular morphogenesisEndothelial cellsMouse glomerular endothelial cellsIntegrin activityGuidance proteinsEndothelial cell proliferationIntegrin inhibitionCaspase-3Cell proliferationSitu markerTube formationSemaphorin 3CPhosphorylationGrowth factorAutocrine class 3 semaphorin system regulates slit diaphragm proteins and podocyte survival
Guan F, Villegas G, Teichman J, Mundel P, Tufro A. Autocrine class 3 semaphorin system regulates slit diaphragm proteins and podocyte survival. Kidney International 2006, 69: 1564-1569. PMID: 16541019, DOI: 10.1038/sj.ki.5000313.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsApoptosisAutocrine CommunicationCells, CulturedCytoskeletal ProteinsDown-RegulationIntracellular Signaling Peptides and ProteinsKidney GlomerulusMembrane ProteinsMicePhosphorylationPodocytesProteinsProto-Oncogene Proteins c-aktReceptors, Cell SurfaceReceptors, ImmunologicSemaphorin-3ASemaphorinsConceptsReverse transcriptase-polymerase chain reactionClass 3 semaphorinsPodocyte survivalSlit diaphragm proteinsSema 3APodocyte apoptosisDiaphragm proteinsTranscriptase-polymerase chain reactionDifferentiated podocytesSema 3A.Activity markersAkt survival pathwayDifferentiated mouse podocytesSemaphorin 3ASemaphorin 3BGuidance proteinsMouse podocytesPodocytesMRNA levelsSurvival pathwaysUndetectable levelsChain reactionPlexin-A1SurvivalSemaphorin Regulation