2023
MPL36, a major plasminogen (PLG) receptor in pathogenic Leptospira, has an essential role during infection
Zhu W, Passalia F, Hamond C, Abe C, Ko A, Barbosa A, Wunder E. MPL36, a major plasminogen (PLG) receptor in pathogenic Leptospira, has an essential role during infection. PLOS Pathogens 2023, 19: e1011313. PMID: 37486929, PMCID: PMC10399853, DOI: 10.1371/journal.ppat.1011313.Peer-Reviewed Original ResearchConceptsOuter membrane proteinsBacterial outer membrane proteinsC-terminal regionSurface-exposed regionsLeptospiral pathogenesisMDCK cell monolayersMembrane proteinsPathogenic LeptospiraImportant virulence factorMutant strainRecombinant proteinsPathogen's abilityVirulence mechanismsLysine residuesKoch's molecular postulatesBinding proteinPlasminogen receptorPathogen disseminationPathogenic spirochetesVirulence factorsProteinBacterial pathogensEssential roleMutantsHost tissues
2021
Effect of Sewerage on the Contamination of Soil with Pathogenic Leptospira in Urban Slums
Casanovas-Massana A, Souza F, Curry M, de Oliveira D, de Oliveira AS, Eyre MT, Santiago D, Santos M, Serra RMR, Lopes E, Xavier BI, Diggle PJ, Wunder EA, Reis MG, Ko AI, Costa F. Effect of Sewerage on the Contamination of Soil with Pathogenic Leptospira in Urban Slums. Environmental Science And Technology 2021, 55: 15882-15890. PMID: 34767339, PMCID: PMC9302045, DOI: 10.1021/acs.est.1c04916.Peer-Reviewed Original ResearchConceptsUrban slumsHuman leptospirosisOpen counterpartPathogenic LeptospiraLeptospirosisZoonotic diseaseFecal markersGenetic Evidence for a Potential Environmental Pathway to Spillover Infection of Rat-Borne Leptospirosis
Casanovas-Massana A, de Oliveira D, Schneider AG, Begon M, Childs JE, Costa F, Reis MG, Ko AI, Wunder EA. Genetic Evidence for a Potential Environmental Pathway to Spillover Infection of Rat-Borne Leptospirosis. The Journal Of Infectious Diseases 2021, 225: 130-134. PMID: 34139761, PMCID: PMC8730487, DOI: 10.1093/infdis/jiab323.Peer-Reviewed Original ResearchConceptsPotential environmental pathwaysEnvironmental reservoirsSpillover infectionsEnvironmental pathwaysHuman leptospirosis casesSevere leptospirosisLeptospirosis infectionLeptospirosis casesMolecular epidemiologyGenetic evidencePathogenic LeptospiraLeptospira interrogansInfectionLeptospirosisUrban slumsSpillover pathwaysRat specimensReservoirPathwayUrban communitiesCommunityEpidemiology
2019
Seroprevalence, Risk Factors, and Rodent Reservoirs of Leptospirosis in an Urban Community of Puerto Rico, 2015
Briskin EA, Casanovas-Massana A, Ryff KR, Morales-Estrada S, Hamond C, Perez-Rodriguez NM, Benavidez KM, Weinberger DM, Castro-Arellano I, Wunder EA, Sharp TM, Rivera-Garcia B, Ko AI. Seroprevalence, Risk Factors, and Rodent Reservoirs of Leptospirosis in an Urban Community of Puerto Rico, 2015. The Journal Of Infectious Diseases 2019, 220: 1489-1497. PMID: 31342075, PMCID: PMC6761939, DOI: 10.1093/infdis/jiz339.Peer-Reviewed Original ResearchConceptsRisk factorsAnti-Leptospira antibodiesBurden of leptospirosisRisk of infectionMicroscopic agglutination testCross-sectional surveyQuantitative polymerase chain reactionSeropositive individualsPrevious infectionPolymerase chain reactionLeptospira infectionLeptospira carriageSerogroup IcterohaemorrhagiaeHigh titersStudy participantsAgglutination testLeptospira exposurePathogenic LeptospiraInfectionRodent reservoirsChain reactionAntibodiesRodent trappingSeroprevalenceCommunity sites
2003
Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira
Lüdtke C, Coutinho M, Jouglard S, Moreira C, Fernandes C, Brod C, Haake D, Ko A, Dellagostin O, Aleixo J. Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira. Brazilian Journal Of Microbiology 2003, 34: 1-4. DOI: 10.1590/s1517-83822003000500001.Peer-Reviewed Original ResearchBALB/c miceMonoclonal antibodiesPathogenic LeptospiraSP2/OWhole-cell antigen preparationsIndirect ELISANon-pathogenic serovarsIgG2b isotypesC miceSpleen cellsAntigen preparationsMAb specificityDiagnostic testsPathogenic serovarsRecombinant LipL32Non-pathogenic LeptospiraMAbsLeptospiraELISAHybridoma productionAntibodiesLipL32SerovarsOuter membrane proteinsCells