2019
The Impact of Single and Multiple Sperm Abnormalities and Low-Level Leukocytospermia on Sperm DNA
Alshahrani S, Ahmad G, Khan H, Ayaz A, Ali A. The Impact of Single and Multiple Sperm Abnormalities and Low-Level Leukocytospermia on Sperm DNA. Journal Of Men's Health 2019, 15: e51-e58. DOI: 10.22374/jomh.v15i2.144.Peer-Reviewed Original ResearchStandard sperm parametersSperm parametersDNA damageAnalysis of semen characteristicsCohort of infertile menSperm DNA testingSperm DNA damageDNA fragmentation analysisLevel of leukocytospermiaNick end labelingSperm motilityInfertile menSemen characteristicsSpermSperm abnormalitiesSperm DNASemen abnormalitiesPercentage of DNA damageRetrospective analysisFragment analysisDNAAbnormal parametersART treatmentEnd labelingDNA testing
2018
Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species
Ayaz A, Agarwal A, Sharma R, Kothandaraman N, Cakar Z, Sikka S. Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species. Andrologia 2018, 50: e13015. PMID: 29656391, DOI: 10.1111/and.13015.Peer-Reviewed Original ResearchMeSH KeywordsAdultAmyloid beta-Protein PrecursorAntigens, NeoplasmF-Box ProteinsHSP70 Heat-Shock ProteinsHumansInfertility, MaleMaleMAP Kinase Kinase Kinase 3Nerve Tissue ProteinsNitric Oxide Synthase Type IIPeptidyl-Dipeptidase AProteomicsReactive Oxygen SpeciesRibosomal ProteinsSmall Ubiquitin-Related Modifier ProteinsSpermatozoaTestisUbiquitinsConceptsReactive oxygen speciesSperm proteinsMale infertilityAngiotensin-converting enzymePost-acrosomal regionPoor sperm qualityLevels of reactive oxygen speciesSperm qualityTesticular environmentInfertile menHeat shock proteinsProteomic analysisHSPA2SpermatozoaExpression profilesOxidative stressRPS27aProteinOxygen speciesMAP3K3Western blottingNeck areaRisk factorsUBL4ASpermChapter 23 Standardized Semen Analysis and Quality Control Management for Multicenter Male Reproductive Toxicology Clinical Trials
Sikka S, Ayaz A. Chapter 23 Standardized Semen Analysis and Quality Control Management for Multicenter Male Reproductive Toxicology Clinical Trials. 2018, 371-386. DOI: 10.1016/b978-0-12-801299-4.00023-2.Peer-Reviewed Original ResearchSemen analysisReproductive failureVariable predictive valueIncidence of reproductive failureHormonal evaluationMen's reproductive healthExposure to endocrine-disrupting chemicalsSemen samplesClinical trialsIncreased incidencePredictive valueLaboratory evaluationReproductive healthTobacco smokeFood additivesNonstandardized techniqueReproductive endocrinologyToxicity trialsSemenStress factorsControl managementEndocrine-disrupting chemicalsLack of standardizationGenetic issuesCounting chamberChapter 21 Impact of Environmental Factors on the Genomics and Proteomics Landscapes of Male Infertility
Ayaz A, Kothandaraman N, Henkel R, Sikka S. Chapter 21 Impact of Environmental Factors on the Genomics and Proteomics Landscapes of Male Infertility. 2018, 335-353. DOI: 10.1016/b978-0-12-801299-4.00021-9.Peer-Reviewed Original ResearchGenetic materialMale infertilityAnalysis of posttranslational modificationsSperm genetic materialSperm epigenomeFunctional genomicsPosttranslational modificationsEnvironmental factorsMicroarray technologyProteomic landscapeGlobal proteinGenetic changesReproductive cellsCritical proteinsDNA structureBioinformatics applicationsProteomic techniquesAdverse effects of environmentGenomeProteinProteomicsDNAImpact of environmental factorsHigh-resolution assessmentInfertile couples
2017
Reconstruction of mammalian oocytes by germinal vesicle transfer: A systematic review
Darbandi S, Darbandi M, Khorram Khorshid H, Shirazi A, Sadeghi M, Agarwal A, Al-Hasani S, Naderi M, Ayaz A, i Akhondi M. Reconstruction of mammalian oocytes by germinal vesicle transfer: A systematic review. International Journal Of Reproductive BioMedicine (IJRM) 2017, 15: 601-612. DOI: 10.29252/ijrm.15.10.1.Peer-Reviewed Original ResearchReconstruction of mammalian oocytes by germinal vesicle transfer: A systematic review
Darbandi S, Darbandi M, Khorram Khorshid H, Shirazi A, Sadeghi M, Agarwal A, Al-Hasani S, Naderi M, Ayaz A, Akhondi M. Reconstruction of mammalian oocytes by germinal vesicle transfer: A systematic review. International Journal Of Reproductive BioMedicine (IJRM) 2017, 15: 601-612. PMID: 29387825, PMCID: PMC5767640, DOI: 10.29252/ijrm.15.10.2.Peer-Reviewed Original ResearchGerminal vesicle stage oocytesGerminal vesicle transferNuclear transfer proceduresAssisted reproductive techniquesMitochondrial problemsVesicle transferNuclear transfer techniqueMammalian speciesPotential clinical applicationsOocyte activationMammalian oocytesReproductive techniquesStage oocytesSurvival rateEffective treatmentOocytesOlder oocytesClinical applicationTransfer proceduresTransfer techniqueSpeciesDifferential expression and localization of ACE and MAP3K3 in oxidative stress related male infertility
Ayaz A, Kothandaraman N, Agarwal A, Cakar Z, Sikka S, Sabanegh E, Sharma R. Differential expression and localization of ACE and MAP3K3 in oxidative stress related male infertility. Fertility And Sterility 2017, 108: e317-e318. DOI: 10.1016/j.fertnstert.2017.07.941.Peer-Reviewed Original ResearchMulti-center evaluation of oxidation reduction potential assay in the infertile male
Agarwal A, Chandrakumar R, Arafa M, Elbardisi H, Okada H, Suzuki K, Homa S, Killeen A, Balaban B, Ayaz A, Saleh R, Armagan A, Roychoudhury S, Sikka S. Multi-center evaluation of oxidation reduction potential assay in the infertile male. Fertility And Sterility 2017, 108: e317. DOI: 10.1016/j.fertnstert.2017.07.939.Peer-Reviewed Original Research
2016
Infertile men have a redox imbalance that distinguishes them from fertile men
Ayaz A, Bjugstad K, Bar-Or D, Armagan A. Infertile men have a redox imbalance that distinguishes them from fertile men. Fertility And Sterility 2016, 106: e235. DOI: 10.1016/j.fertnstert.2016.07.678.Peer-Reviewed Original ResearchPost-Translational Modifications in sperm Proteome: The Chemistry of Proteome diversifications in the Pathophysiology of male factor infertility
Samanta L, Swain N, Ayaz A, Venugopal V, Agarwal A. Post-Translational Modifications in sperm Proteome: The Chemistry of Proteome diversifications in the Pathophysiology of male factor infertility. Biochimica Et Biophysica Acta 2016, 1860: 1450-1465. PMID: 27062907, DOI: 10.1016/j.bbagen.2016.04.001.Peer-Reviewed Original ResearchConceptsPost-translational modificationsSperm proteinsSperm functionOccurrence of post-translational modificationsExpressed protein complementHuman sperm proteinsProtein synthesis machineryAnalysis of post-translational modificationsComplex interaction networksProteome diversificationSperm proteomeFemale reproductive tractHuman spermatozoaProtein complementStructure-function relationshipsSynthesis machineryInteraction networkProtein stabilityMale infertilitySpermMale factor infertilitySpermatozoaProtein profilesDifferential occurrenceFertility potential
2015
Proteomic analysis of seminal plasma of infertile men with differing levels of reactive oxygen species reveals defective protein turnover
Agarwal A, Ayaz A, Sharma R, Samanta L, Assidi M, Gupta S, Sabanegh E. Proteomic analysis of seminal plasma of infertile men with differing levels of reactive oxygen species reveals defective protein turnover. Fertility And Sterility 2015, 104: e144. DOI: 10.1016/j.fertnstert.2015.07.445.Peer-Reviewed Original ResearchComparative proteomic pathway analysis of different phenotypes of ejaculated spermatozoa in fertile and infertile men
Agarwal A, Cui Z, Sharma R, Samanta L, Gupta S, Ayaz A, Sabanegh E. Comparative proteomic pathway analysis of different phenotypes of ejaculated spermatozoa in fertile and infertile men. Fertility And Sterility 2015, 104: e297-e298. DOI: 10.1016/j.fertnstert.2015.07.931.Peer-Reviewed Original ResearchWhat makes some ROS-positive men fertile? - A comparative proteomic study
Sharma R, Agarwal A, Ayaz A, Samanta L, Abu-Elmagd M, Gupta S, Sabanegh E. What makes some ROS-positive men fertile? - A comparative proteomic study. Fertility And Sterility 2015, 104: e144-e145. DOI: 10.1016/j.fertnstert.2015.07.446.Peer-Reviewed Original ResearchComparative proteomic network signatures in seminal plasma of infertile men as a function of reactive oxygen species
Agarwal A, Ayaz A, Samanta L, Sharma R, Assidi M, Abuzenadah A, Sabanegh E. Comparative proteomic network signatures in seminal plasma of infertile men as a function of reactive oxygen species. Clinical Proteomics 2015, 12: 23. PMID: 26321892, PMCID: PMC4552280, DOI: 10.1186/s12014-015-9094-5.Peer-Reviewed Original ResearchMembrane metallo-endopeptidaseInfertile menSeminal plasmaSperm functionFertile menLTQ-Orbitrap Elite hybrid mass spectrometer systemProtein foldingNatural reservoir of antioxidantsMale infertilityROS levelsComparison to fertile controlsSeminal plasma of infertile menPost-translational modification of proteinsHigh ROS groupImpairment of sperm functionPathology of male infertilitySeminal plasma proteinsDiagnosis of male infertilityFunction of reactive oxygen speciesMotility of spermPost-translational modificationsFertile groupModification of proteinsInfertile patient groupsOxygen speciesOxidative Stress and Overview of Pediatric Disease Biomarkers
Ayaz A. Oxidative Stress and Overview of Pediatric Disease Biomarkers. Journal Of Pediatric Biochemistry 2015, 05: 008-011. DOI: 10.1055/s-0035-1554784.Peer-Reviewed Original ResearchSpermatozoa protein alterations in infertile men with bilateral varicocele
Agarwal A, Sharma R, Durairajanayagam D, Cui Z, Ayaz A, Gupta S, Willard B, Gopalan B, Sabanegh E. Spermatozoa protein alterations in infertile men with bilateral varicocele. Asian Journal Of Andrology 2015, 18: 43-53. PMID: 25999357, PMCID: PMC4736356, DOI: 10.4103/1008-682x.153848.Peer-Reviewed Original ResearchConceptsInfertile menBilateral varicoceleFertile menAssociated with metabolic processesSperm-zona bindingVaricocele casesFunctional proteomic profilingVaricocele-associated male infertilityImmune system processPresence of DEPSperm-zonaSperm functionFertile control groupCells of patientsFertile donorsEnzyme regulationIdentified proteinsOxidoreductase activityMale infertilitySpermatozoa cellsProteomic analysisWestern blot analysisStress responseMetabolic processesProteomic profilingMP76-16 IMPACT OF PRECISE MODULATION OF REACTIVE OXYGEN SPECIES LEVELS ON SPERMATOZOA PROTEINS IN INFERTILE MEN
Sharma R, Agarwal A, Ayaz A, Sabanegh E. MP76-16 IMPACT OF PRECISE MODULATION OF REACTIVE OXYGEN SPECIES LEVELS ON SPERMATOZOA PROTEINS IN INFERTILE MEN. Journal Of Urology 2015, 193: e990. DOI: 10.1016/j.juro.2015.02.2801.Peer-Reviewed Original ResearchDifferential Proteomic Profiling of Spermatozoal Proteins of Infertile Men With Unilateral or Bilateral Varicocele
Agarwal A, Sharma R, Durairajanayagam D, Cui Z, Ayaz A, Gupta S, Willard B, Gopalan B, Sabanegh E. Differential Proteomic Profiling of Spermatozoal Proteins of Infertile Men With Unilateral or Bilateral Varicocele. Urology 2015, 85: 580-588. PMID: 25733269, DOI: 10.1016/j.urology.2014.11.030.Peer-Reviewed Original ResearchConceptsSperm protein profilePost-translational modificationsInfertile menRoutine sperm parametersProtein profilesSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisFunctional proteomic profilingGel analysis softwareVaricocele-associated male infertilitySperm deoxyribonucleic acid fragmentationComparing spectral countsVaricocele samplesDeoxyribonucleic acid fragmentationProtein foldingSperm parametersDifferential proteomic profilingProtein ubiquitinationCellular functionsSignal transductionGel electrophoresisExpressed proteinsMale infertilityReactive oxygen speciesMajor protein alterations in spermatozoa from infertile men with unilateral varicocele
Agarwal A, Sharma R, Durairajanayagam D, Ayaz A, Cui Z, Willard B, Gopalan B, Sabanegh E. Major protein alterations in spermatozoa from infertile men with unilateral varicocele. Reproductive Biology And Endocrinology 2015, 13: 8. PMID: 25890347, PMCID: PMC4383193, DOI: 10.1186/s12958-015-0007-2.Peer-Reviewed Original ResearchConceptsInfertile menVaricocele groupLTQ-Orbitrap Elite hybrid mass spectrometer systemAcquisition of sperm motilityPost-translational modification proteinsFertile groupCause of male factor infertilitySmall molecule biochemistryAcrosome reactionSperm maturationUnilateral varicoceleFunctional annotationModification proteinsReactive oxygen species levelsSperm motilityResultsSperm concentrationBioinformatics toolsSpecies levelFertile menSignal transductionProteomic changesFunctional pathwaysBioinformatics analysisAnalyzed proteinsGene expressionImpact of precise modulation of reactive oxygen species levels on spermatozoa proteins in infertile men
Ayaz A, Agarwal A, Sharma R, Arafa M, Elbardisi H, Cui Z. Impact of precise modulation of reactive oxygen species levels on spermatozoa proteins in infertile men. Clinical Proteomics 2015, 12: 4. PMID: 25972767, PMCID: PMC4429661, DOI: 10.1186/1559-0275-12-4.Peer-Reviewed Original ResearchReactive oxygen species levelsInfertile menReactive oxygen speciesLevels of reactive oxygen speciesMale infertilityROS groupHigher ROS levelsHigher reactive oxygen speciesFertile menExpression of sperm proteinsHigh ROS groupPathology of male infertilityROS levelsGlobal proteomic analysisSperm proteinsSpermatozoa proteinsReactive oxygen species groupIn-gel digestionBioinformatics toolsSpecies levelCellular processesExpressed proteinsReproductive functionProteomic analysisIn-gel