2018
Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species
Ayaz A, Agarwal A, Sharma R, Kothandaraman N, Cakar Z, Sikka S. Proteomic analysis of sperm proteins in infertile men with high levels of reactive oxygen species. Andrologia 2018, 50: e13015. PMID: 29656391, DOI: 10.1111/and.13015.Peer-Reviewed Original ResearchMeSH KeywordsAdultAmyloid beta-Protein PrecursorAntigens, NeoplasmF-Box ProteinsHSP70 Heat-Shock ProteinsHumansInfertility, MaleMaleMAP Kinase Kinase Kinase 3Nerve Tissue ProteinsNitric Oxide Synthase Type IIPeptidyl-Dipeptidase AProteomicsReactive Oxygen SpeciesRibosomal ProteinsSmall Ubiquitin-Related Modifier ProteinsSpermatozoaTestisUbiquitinsConceptsReactive oxygen speciesSperm proteinsMale infertilityAngiotensin-converting enzymePost-acrosomal regionPoor sperm qualityLevels of reactive oxygen speciesSperm qualityTesticular environmentInfertile menHeat shock proteinsProteomic analysisHSPA2SpermatozoaExpression profilesOxidative stressRPS27aProteinOxygen speciesMAP3K3Western blottingNeck areaRisk factorsUBL4ASperm
2016
Post-Translational Modifications in sperm Proteome: The Chemistry of Proteome diversifications in the Pathophysiology of male factor infertility
Samanta L, Swain N, Ayaz A, Venugopal V, Agarwal A. Post-Translational Modifications in sperm Proteome: The Chemistry of Proteome diversifications in the Pathophysiology of male factor infertility. Biochimica Et Biophysica Acta 2016, 1860: 1450-1465. PMID: 27062907, DOI: 10.1016/j.bbagen.2016.04.001.Peer-Reviewed Original ResearchConceptsPost-translational modificationsSperm proteinsSperm functionOccurrence of post-translational modificationsExpressed protein complementHuman sperm proteinsProtein synthesis machineryAnalysis of post-translational modificationsComplex interaction networksProteome diversificationSperm proteomeFemale reproductive tractHuman spermatozoaProtein complementStructure-function relationshipsSynthesis machineryInteraction networkProtein stabilityMale infertilitySpermMale factor infertilitySpermatozoaProtein profilesDifferential occurrenceFertility potential
2015
Spermatozoa protein alterations in infertile men with bilateral varicocele
Agarwal A, Sharma R, Durairajanayagam D, Cui Z, Ayaz A, Gupta S, Willard B, Gopalan B, Sabanegh E. Spermatozoa protein alterations in infertile men with bilateral varicocele. Asian Journal Of Andrology 2015, 18: 43-53. PMID: 25999357, PMCID: PMC4736356, DOI: 10.4103/1008-682x.153848.Peer-Reviewed Original ResearchMeSH KeywordsAdultCase-Control StudiesHumansInfertility, MaleMaleProteinsSpermatozoaVaricoceleYoung AdultConceptsInfertile menBilateral varicoceleFertile menAssociated with metabolic processesSperm-zona bindingVaricocele casesFunctional proteomic profilingVaricocele-associated male infertilityImmune system processPresence of DEPSperm-zonaSperm functionFertile control groupCells of patientsFertile donorsEnzyme regulationIdentified proteinsOxidoreductase activityMale infertilitySpermatozoa cellsProteomic analysisWestern blot analysisStress responseMetabolic processesProteomic profilingDifferential Proteomic Profiling of Spermatozoal Proteins of Infertile Men With Unilateral or Bilateral Varicocele
Agarwal A, Sharma R, Durairajanayagam D, Cui Z, Ayaz A, Gupta S, Willard B, Gopalan B, Sabanegh E. Differential Proteomic Profiling of Spermatozoal Proteins of Infertile Men With Unilateral or Bilateral Varicocele. Urology 2015, 85: 580-588. PMID: 25733269, DOI: 10.1016/j.urology.2014.11.030.Peer-Reviewed Original ResearchConceptsSperm protein profilePost-translational modificationsInfertile menRoutine sperm parametersProtein profilesSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisFunctional proteomic profilingGel analysis softwareVaricocele-associated male infertilitySperm deoxyribonucleic acid fragmentationComparing spectral countsVaricocele samplesDeoxyribonucleic acid fragmentationProtein foldingSperm parametersDifferential proteomic profilingProtein ubiquitinationCellular functionsSignal transductionGel electrophoresisExpressed proteinsMale infertilityReactive oxygen speciesMajor protein alterations in spermatozoa from infertile men with unilateral varicocele
Agarwal A, Sharma R, Durairajanayagam D, Ayaz A, Cui Z, Willard B, Gopalan B, Sabanegh E. Major protein alterations in spermatozoa from infertile men with unilateral varicocele. Reproductive Biology And Endocrinology 2015, 13: 8. PMID: 25890347, PMCID: PMC4383193, DOI: 10.1186/s12958-015-0007-2.Peer-Reviewed Original ResearchConceptsInfertile menVaricocele groupLTQ-Orbitrap Elite hybrid mass spectrometer systemAcquisition of sperm motilityPost-translational modification proteinsFertile groupCause of male factor infertilitySmall molecule biochemistryAcrosome reactionSperm maturationUnilateral varicoceleFunctional annotationModification proteinsReactive oxygen species levelsSperm motilityResultsSperm concentrationBioinformatics toolsSpecies levelFertile menSignal transductionProteomic changesFunctional pathwaysBioinformatics analysisAnalyzed proteinsGene expression
2014
Infertile men older than 40 years are at higher risk of sperm DNA damage
Alshahrani S, Agarwal A, Assidi M, Abuzenadah A, Durairajanayagam D, Ayaz A, Sharma R, Sabanegh E. Infertile men older than 40 years are at higher risk of sperm DNA damage. Reproductive Biology And Endocrinology 2014, 12: 103. PMID: 25410314, PMCID: PMC4258051, DOI: 10.1186/1477-7827-12-103.Peer-Reviewed Original ResearchConceptsSperm DNA damageRisk of sperm DNA damageInfertile menReactive oxygen speciesDNA damagePercentage of sperm DNA fragmentationLevels of sperm DNA damageSemen analysisSeminal reactive oxygen speciesPaternal ageSperm DNA fragmentationAge groupsAssociated with higher riskPaternal age >DNA fragmentationSperm parametersAdvanced paternal ageSemen parametersSemen qualityEffect of paternal ageSpermRetrospective studyGroup BMethodsA totalGroup A