Comparative proteomic network signatures in seminal plasma of infertile men as a function of reactive oxygen species
Agarwal A, Ayaz A, Samanta L, Sharma R, Assidi M, Abuzenadah A, Sabanegh E. Comparative proteomic network signatures in seminal plasma of infertile men as a function of reactive oxygen species. Clinical Proteomics 2015, 12: 23. PMID: 26321892, PMCID: PMC4552280, DOI: 10.1186/s12014-015-9094-5.Peer-Reviewed Original ResearchMembrane metallo-endopeptidaseInfertile menSeminal plasmaSperm functionFertile menLTQ-Orbitrap Elite hybrid mass spectrometer systemProtein foldingNatural reservoir of antioxidantsMale infertilityROS levelsComparison to fertile controlsSeminal plasma of infertile menPost-translational modification of proteinsHigh ROS groupImpairment of sperm functionPathology of male infertilitySeminal plasma proteinsDiagnosis of male infertilityFunction of reactive oxygen speciesMotility of spermPost-translational modificationsFertile groupModification of proteinsInfertile patient groupsOxygen speciesSpermatozoa protein alterations in infertile men with bilateral varicocele
Agarwal A, Sharma R, Durairajanayagam D, Cui Z, Ayaz A, Gupta S, Willard B, Gopalan B, Sabanegh E. Spermatozoa protein alterations in infertile men with bilateral varicocele. Asian Journal Of Andrology 2015, 18: 43-53. PMID: 25999357, PMCID: PMC4736356, DOI: 10.4103/1008-682x.153848.Peer-Reviewed Original ResearchConceptsInfertile menBilateral varicoceleFertile menAssociated with metabolic processesSperm-zona bindingVaricocele casesFunctional proteomic profilingVaricocele-associated male infertilityImmune system processPresence of DEPSperm-zonaSperm functionFertile control groupCells of patientsFertile donorsEnzyme regulationIdentified proteinsOxidoreductase activityMale infertilitySpermatozoa cellsProteomic analysisWestern blot analysisStress responseMetabolic processesProteomic profilingMajor protein alterations in spermatozoa from infertile men with unilateral varicocele
Agarwal A, Sharma R, Durairajanayagam D, Ayaz A, Cui Z, Willard B, Gopalan B, Sabanegh E. Major protein alterations in spermatozoa from infertile men with unilateral varicocele. Reproductive Biology And Endocrinology 2015, 13: 8. PMID: 25890347, PMCID: PMC4383193, DOI: 10.1186/s12958-015-0007-2.Peer-Reviewed Original ResearchConceptsInfertile menVaricocele groupLTQ-Orbitrap Elite hybrid mass spectrometer systemAcquisition of sperm motilityPost-translational modification proteinsFertile groupCause of male factor infertilitySmall molecule biochemistryAcrosome reactionSperm maturationUnilateral varicoceleFunctional annotationModification proteinsReactive oxygen species levelsSperm motilityResultsSperm concentrationBioinformatics toolsSpecies levelFertile menSignal transductionProteomic changesFunctional pathwaysBioinformatics analysisAnalyzed proteinsGene expressionImpact of precise modulation of reactive oxygen species levels on spermatozoa proteins in infertile men
Ayaz A, Agarwal A, Sharma R, Arafa M, Elbardisi H, Cui Z. Impact of precise modulation of reactive oxygen species levels on spermatozoa proteins in infertile men. Clinical Proteomics 2015, 12: 4. PMID: 25972767, PMCID: PMC4429661, DOI: 10.1186/1559-0275-12-4.Peer-Reviewed Original ResearchReactive oxygen species levelsInfertile menReactive oxygen speciesLevels of reactive oxygen speciesMale infertilityROS groupHigher ROS levelsHigher reactive oxygen speciesFertile menExpression of sperm proteinsHigh ROS groupPathology of male infertilityROS levelsGlobal proteomic analysisSperm proteinsSpermatozoa proteinsReactive oxygen species groupIn-gel digestionBioinformatics toolsSpecies levelCellular processesExpressed proteinsReproductive functionProteomic analysisIn-gel