Major protein alterations in spermatozoa from infertile men with unilateral varicocele
Agarwal A, Sharma R, Durairajanayagam D, Ayaz A, Cui Z, Willard B, Gopalan B, Sabanegh E. Major protein alterations in spermatozoa from infertile men with unilateral varicocele. Reproductive Biology And Endocrinology 2015, 13: 8. PMID: 25890347, PMCID: PMC4383193, DOI: 10.1186/s12958-015-0007-2.Peer-Reviewed Original ResearchConceptsInfertile menVaricocele groupLTQ-Orbitrap Elite hybrid mass spectrometer systemAcquisition of sperm motilityPost-translational modification proteinsFertile groupCause of male factor infertilitySmall molecule biochemistryAcrosome reactionSperm maturationUnilateral varicoceleFunctional annotationModification proteinsReactive oxygen species levelsSperm motilityResultsSperm concentrationBioinformatics toolsSpecies levelFertile menSignal transductionProteomic changesFunctional pathwaysBioinformatics analysisAnalyzed proteinsGene expressionImpact of precise modulation of reactive oxygen species levels on spermatozoa proteins in infertile men
Ayaz A, Agarwal A, Sharma R, Arafa M, Elbardisi H, Cui Z. Impact of precise modulation of reactive oxygen species levels on spermatozoa proteins in infertile men. Clinical Proteomics 2015, 12: 4. PMID: 25972767, PMCID: PMC4429661, DOI: 10.1186/1559-0275-12-4.Peer-Reviewed Original ResearchReactive oxygen species levelsInfertile menReactive oxygen speciesLevels of reactive oxygen speciesMale infertilityROS groupHigher ROS levelsHigher reactive oxygen speciesFertile menExpression of sperm proteinsHigh ROS groupPathology of male infertilityROS levelsGlobal proteomic analysisSperm proteinsSpermatozoa proteinsReactive oxygen species groupIn-gel digestionBioinformatics toolsSpecies levelCellular processesExpressed proteinsReproductive functionProteomic analysisIn-gel