2017
Molecular and cellular reorganization of neural circuits in the human lineage
Sousa AMM, Zhu Y, Raghanti MA, Kitchen RR, Onorati M, Tebbenkamp ATN, Stutz B, Meyer KA, Li M, Kawasawa YI, Liu F, Perez RG, Mele M, Carvalho T, Skarica M, Gulden FO, Pletikos M, Shibata A, Stephenson AR, Edler MK, Ely JJ, Elsworth JD, Horvath TL, Hof PR, Hyde TM, Kleinman JE, Weinberger DR, Reimers M, Lifton RP, Mane SM, Noonan JP, State MW, Lein ES, Knowles JA, Marques-Bonet T, Sherwood CC, Gerstein MB, Sestan N. Molecular and cellular reorganization of neural circuits in the human lineage. Science 2017, 358: 1027-1032. PMID: 29170230, PMCID: PMC5776074, DOI: 10.1126/science.aan3456.Peer-Reviewed Original ResearchConceptsSingle-cell transcriptomic dataDistinct functional categoriesDistinct cell typesBiosynthesis genesTranscriptome sequencingHuman lineageTranscriptomic dataFunctional categoriesCellular reorganizationExpression differencesPhylogenetic reorganizationFunctional analysisCell typesGenesCellular featuresCellular differencesHuman specificityNeural circuitsLineagesMultiple levelsReorganizationSequencingHumansChimpanzeesAdult humans
2016
Zika Virus Disrupts Phospho-TBK1 Localization and Mitosis in Human Neuroepithelial Stem Cells and Radial Glia
Onorati M, Li Z, Liu F, Sousa AMM, Nakagawa N, Li M, Dell’Anno M, Gulden FO, Pochareddy S, Tebbenkamp AT, Han W, Pletikos M, Gao T, Zhu Y, Bichsel C, Varela L, Szigeti-Buck K, Lisgo S, Zhang Y, Testen A, Gao XB, Mlakar J, Popovic M, Flamand M, Strittmatter SM, Kaczmarek LK, Anton ES, Horvath TL, Lindenbach BD, Sestan N. Zika Virus Disrupts Phospho-TBK1 Localization and Mitosis in Human Neuroepithelial Stem Cells and Radial Glia. Cell Reports 2016, 16: 2576-2592. PMID: 27568284, PMCID: PMC5135012, DOI: 10.1016/j.celrep.2016.08.038.Peer-Reviewed Original ResearchMeSH KeywordsAxl Receptor Tyrosine KinaseBrainCell DeathCentrosomeFetusGene Expression ProfilingHumansImmunity, InnateMicrocephalyMitochondriaMitosisNeocortexNeural Stem CellsNeuroepithelial CellsNeurogliaNeuronsNeuroprotective AgentsNucleosidesPhosphorylationProtein Kinase InhibitorsProtein Serine-Threonine KinasesProto-Oncogene ProteinsReceptor Protein-Tyrosine KinasesSpinal CordTranscription, GeneticVirus ReplicationZika VirusZika Virus InfectionConceptsRadial glial cellsNES cellsNeuroepithelial stem cellsZIKV infectionFetal brain slicesStem cellsEarly human neurodevelopmentHuman neuroepithelial stem cellsHuman neural stem cellsCell deathSingle-cell RNA-seqNeural stem cellsNeurodevelopment defectsZIKV replicationGlial cellsBrain slicesPotential treatmentRadial gliaZika virusPhospho-TBK1Neurodevelopmental defectsRNA-seqSupernumerary centrosomesNucleoside analoguesHuman neurodevelopment
2002
ER-X: A Novel, Plasma Membrane-Associated, Putative Estrogen Receptor That Is Regulated during Development and after Ischemic Brain Injury
Toran-Allerand CD, Guan X, MacLusky NJ, Horvath TL, Diano S, Singh M, Connolly ES, Nethrapalli S, Tinnikov AA. ER-X: A Novel, Plasma Membrane-Associated, Putative Estrogen Receptor That Is Regulated during Development and after Ischemic Brain Injury. Journal Of Neuroscience 2002, 22: 8391-8401. PMID: 12351713, PMCID: PMC6757764, DOI: 10.1523/jneurosci.22-19-08391.2002.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding, CompetitiveBlotting, WesternBrain IschemiaCaveolaeCell MembraneCell-Free SystemCells, CulturedCholesterolEnzyme ActivationEstradiolGene Expression Regulation, DevelopmentalIonophoresMiceMice, KnockoutMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesNeocortexNeuronsReceptors, EstrogenRNA, MessengerSignal TransductionSubcellular FractionsSubstrate SpecificityConceptsMAPK cascadePlasma membraneExtracellular signal-regulated kinases ERK1Neuronal differentiationPlasma membrane-associatedPlasma membrane-associated ERsProtein kinase isoformsCell-free systemMembrane-associated ERER alphaPutative ERKinases ERK1Cell divisionER betaSustained phosphorylationKinase isoformsERK activationMembrane-associatedGene-disrupted miceNovel mechanismEstrogen receptorUterine plasma membranesInhibitory regulatorIschemic stroke injuryIschemic brain injury