2022
Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling
Hsu JC, Pawlak JB, Laurent-Rolle M, Cresswell P. Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling. STAR Protocols 2022, 3: 101654. PMID: 36072758, PMCID: PMC9442383, DOI: 10.1016/j.xpro.2022.101654.Peer-Reviewed Original ResearchConceptsTranslational regulationMammalian cell linesDifferent cell typesDifferent growth conditionsMammalian cellsTranslation activityGene expressionBiological processesCell typesComplete detailsConfocal microscopyCell linesGrowth conditionsPuromycin analoguesCritical roleRegulationCellsProteinLabelingFundamental stepExpressionClick chemistry reactionActivityCytometry
2005
Inhibition of Alpha/Beta Interferon Signaling by the NS4B Protein of Flaviviruses
Muñoz-Jordán JL, Laurent-Rolle M, Ashour J, Martínez-Sobrido L, Ashok M, Lipkin WI, García-Sastre A. Inhibition of Alpha/Beta Interferon Signaling by the NS4B Protein of Flaviviruses. Journal Of Virology 2005, 79: 8004-8013. PMID: 15956546, PMCID: PMC1143737, DOI: 10.1128/jvi.79.13.8004-8013.2005.Peer-Reviewed Original ResearchConceptsSignal peptideCleavable signal peptidePositive-strand RNA virusesInterferon-stimulated response element promoterISRE promoter activationNonstructural protein NS4BB fusion proteinFunction of NS4BResponse element promoterActivation of STAT1Dengue virus NS4BNS2B/NS3Alpha/beta interferonAnti-interferon functionDeletion analysisIFN antagonismN-terminusPromoter activationFusion proteinStructural proteinsNonstructural proteinsInhibition of interferonRNA virusesViral polyprotein processingNS4B protein