2024
Is it time to reduce the length of postgraduate training for physician-scientists in internal medicine?
Gallagher E, Conlin P, Kazmierczak B, Vyas J, Ajijola O, Kontos C, Baiocchi R, Rhee K, Hu P, Isales C, Williams C, Rockey D. Is it time to reduce the length of postgraduate training for physician-scientists in internal medicine? JCI Insight 2024, 9: e178214. PMID: 38775155, PMCID: PMC11141926, DOI: 10.1172/jci.insight.178214.Peer-Reviewed Original ResearchConceptsMedical school graduatesCareer development awardsInternal medicine residency graduatesResidency graduatesRetention of physician-scientistsInternal medicinePostgraduate trainingPhysician-scientistsSchool graduatesK awardsK awardeesMD-PhD graduatesPatient careComplete trainingTraining durationMedian timeMedical knowledgeDevelopment AwardNIH RePORTERTrainingIK2GraduatesAwardeesMedicineCareInterventions to support fellowship application success among predoctoral physician-scientists
Fitzsimonds R, Gorelick F, Kazmierczak B. Interventions to support fellowship application success among predoctoral physician-scientists. JCI Insight 2024, 9: e175857. PMID: 38456505, PMCID: PMC10972582, DOI: 10.1172/jci.insight.175857.Peer-Reviewed Original Research
2023
Structural insights into the career path between pre- and postgraduate physician-scientist training programs
Williams C, Gallagher E, Rockey D, Ajijola O, Hu P, Kazmierczak B, Kontos C, Vyas J, Zaidi M, Rhee K. Structural insights into the career path between pre- and postgraduate physician-scientist training programs. ELife 2023, 12: e87148. PMID: 37782020, PMCID: PMC10545427, DOI: 10.7554/elife.87148.Peer-Reviewed Original Research
2022
Assessment of polymicrobial interactions in bacterial isolates from transfused platelet units associated with sepsis
Kerantzas CA, Merwede J, Snyder EL, Hendrickson JE, Tormey CA, Kazmierczak BI, Peaper DR. Assessment of polymicrobial interactions in bacterial isolates from transfused platelet units associated with sepsis. Transfusion 2022, 62: 2458-2463. PMID: 36178430, PMCID: PMC11472026, DOI: 10.1111/trf.17136.Peer-Reviewed Original ResearchConceptsAcinetobacter calcoaceticus-baumannii complexBlood productsS. saprophyticusPlatelet unitsContaminated blood productsCDC investigationsApheresis platelet productsTransfusion reactionsPolymicrobial contaminationPlatelet productsDisease controlBacterial isolatesStudy designStaphylococcus saprophyticusPolymicrobial interactionsCDC casesSaprophyticusFuture studiesPotential interactionsCommon sourceSepsisIsolatesCasesCoaggregationPearls of wisdom for aspiring physician-scientist residency applicants and program directors
Gallagher EJ, Rockey DC, Kontos CD, Vyas JM, Brass LF, Hu PJ, Isales CM, Ajijola OA, Rathmell WK, Conlin PR, Baiocchi RA, Kazmierczak BI, Akabas MH, Williams CS. Pearls of wisdom for aspiring physician-scientist residency applicants and program directors. JCI Insight 2022, 7: e158467. PMID: 35315364, PMCID: PMC8986063, DOI: 10.1172/jci.insight.158467.Peer-Reviewed Original Research
2020
The Enemy of my Enemy: Bacterial Competition in the Cystic Fibrosis Lung
Kazmierczak BI. The Enemy of my Enemy: Bacterial Competition in the Cystic Fibrosis Lung. Cell Host & Microbe 2020, 28: 502-504. PMID: 33031766, DOI: 10.1016/j.chom.2020.09.009.Peer-Reviewed Original ResearchGlobal chemical effects of the microbiome include new bile-acid conjugations
Quinn RA, Melnik AV, Vrbanac A, Fu T, Patras KA, Christy MP, Bodai Z, Belda-Ferre P, Tripathi A, Chung LK, Downes M, Welch RD, Quinn M, Humphrey G, Panitchpakdi M, Weldon KC, Aksenov A, da Silva R, Avila-Pacheco J, Clish C, Bae S, Mallick H, Franzosa EA, Lloyd-Price J, Bussell R, Thron T, Nelson AT, Wang M, Leszczynski E, Vargas F, Gauglitz JM, Meehan MJ, Gentry E, Arthur TD, Komor AC, Poulsen O, Boland BS, Chang JT, Sandborn WJ, Lim M, Garg N, Lumeng JC, Xavier RJ, Kazmierczak BI, Jain R, Egan M, Rhee KE, Ferguson D, Raffatellu M, Vlamakis H, Haddad GG, Siegel D, Huttenhower C, Mazmanian SK, Evans RM, Nizet V, Knight R, Dorrestein PC. Global chemical effects of the microbiome include new bile-acid conjugations. Nature 2020, 579: 123-129. PMID: 32103176, PMCID: PMC7252668, DOI: 10.1038/s41586-020-2047-9.Peer-Reviewed Original ResearchConceptsChemical interactionChemistryBile acid synthesis genesChemical effectsInflammatory bowel diseaseBile acid conjugatesCompoundsHost bile acidsMolecular familiesBile acid conjugationBowel diseaseGut diseasesMicrobiome dysbiosisConjugationAcidFree miceAmino acid conjugationBile acidsCystic fibrosisX receptorAcid conjugationReduced expressionFurther studiesDiseaseMiceA Screen for Antibiotic Resistance Determinants Reveals a Fitness Cost of the Flagellum in Pseudomonas aeruginosa
Rundell EA, Commodore N, Goodman AL, Kazmierczak BI. A Screen for Antibiotic Resistance Determinants Reveals a Fitness Cost of the Flagellum in Pseudomonas aeruginosa. Journal Of Bacteriology 2020, 202: 10.1128/jb.00682-19. PMID: 31871033, PMCID: PMC7043666, DOI: 10.1128/jb.00682-19.Peer-Reviewed Original ResearchConceptsFlagellar assemblyFitness advantageFitness costsCell envelopeOuter membrane barrier functionOuter membrane biogenesisUnbiased high-throughput approachOuter membrane barrierMembrane barrier functionHigh-throughput approachMembrane biogenesisGram-negative bacteriaFlagellar functionInsertion sequencingOuter membraneGene productsPresence of linezolidPresence of antibioticsAntibiotic resistance determinantsMembrane barrierAntibiotic entryFitnessEfflux pumpsGlycopeptide antibiotic vancomycinGenes
2018
Host suppression of quorum sensing during catheter-associated urinary tract infections
Cole SJ, Hall CL, Schniederberend M, Farrow III JM, Goodson JR, Pesci EC, Kazmierczak BI, Lee VT. Host suppression of quorum sensing during catheter-associated urinary tract infections. Nature Communications 2018, 9: 4436. PMID: 30361690, PMCID: PMC6202348, DOI: 10.1038/s41467-018-06882-y.Peer-Reviewed Original ResearchConceptsCatheter-associated urinary tract infectionsUrinary tract infectionTract infectionsChronic bacterial infectionP. aeruginosaAntibiotic therapyUrinary tractDevice-associated biofilmsMurine modelHost immunityClinical isolatesBacterial infectionsInfectionPseudomonas aeruginosaUrineAeruginosaTherapyRegulated genesImmunityTract
2017
Macrolides selectively inhibit mutant KCNJ5 potassium channels that cause aldosterone-producing adenoma
Scholl UI, Abriola L, Zhang C, Reimer EN, Plummer M, Kazmierczak BI, Zhang J, Hoyer D, Merkel JS, Wang W, Lifton RP. Macrolides selectively inhibit mutant KCNJ5 potassium channels that cause aldosterone-producing adenoma. Journal Of Clinical Investigation 2017, 127: 2739-2750. PMID: 28604387, PMCID: PMC5490757, DOI: 10.1172/jci91733.Peer-Reviewed Original ResearchConceptsAldosterone-producing adenomaAldosterone productionAdrenocortical cancer cell lineKCNJ5 potassium channelPotassium channel KCNJ5Motilin receptor agonistSteroid hormone aldosteroneMillions of casesAPA diagnosisCancer cell linesSevere hypertensionAdrenal glandReceptor agonistBenign tumorsHormone aldosteroneCalcium influxInvasive proceduresKCNJ5 mutationsAdrenocortical cellsNoninvasive diagnosisPotassium channelsMembrane depolarizationDiagnosisCell proliferationCell linesInflammation: A Double-Edged Sword in the Response to Pseudomonas aeruginosa Infection
Lin CK, Kazmierczak BI. Inflammation: A Double-Edged Sword in the Response to Pseudomonas aeruginosa Infection. Journal Of Innate Immunity 2017, 9: 250-261. PMID: 28222444, PMCID: PMC5469373, DOI: 10.1159/000455857.Peer-Reviewed Original ResearchConceptsP. aeruginosa pulmonary infectionInnate immune recognitionPseudomonas aeruginosa infectionInflamed airwaysPulmonary infectionAcute infectionAdjunct therapyChronic infectionBarrier defenseAeruginosa infectionAnatomic sitesPathogen clearanceBacterial productsImmune recognitionInnate immunityInfectionHost outcomesResistant pathogensP. aeruginosa adaptationInflammationP. aeruginosaPathogen persistenceDouble-Edged SwordPseudomonas aeruginosaDefense mechanisms
2016
Rampant Cheating by Pathogens?
Rundell EA, McKeithen-Mead SA, Kazmierczak BI. Rampant Cheating by Pathogens? PLOS Pathogens 2016, 12: e1005792. PMID: 27606630, PMCID: PMC5015830, DOI: 10.1371/journal.ppat.1005792.Peer-Reviewed Original Research
2015
Cross-regulation of Pseudomonas motility systems: the intimate relationship between flagella, pili and virulence
Kazmierczak BI, Schniederberend M, Jain R. Cross-regulation of Pseudomonas motility systems: the intimate relationship between flagella, pili and virulence. Current Opinion In Microbiology 2015, 28: 78-82. PMID: 26476804, PMCID: PMC4688086, DOI: 10.1016/j.mib.2015.07.017.Peer-Reviewed Original ResearchCompartment-Specific and Sequential Role of MyD88 and CARD9 in Chemokine Induction and Innate Defense during Respiratory Fungal Infection
Jhingran A, Kasahara S, Shepardson KM, Junecko BA, Heung LJ, Kumasaka DK, Knoblaugh SE, Lin X, Kazmierczak BI, Reinhart TA, Cramer RA, Hohl TM. Compartment-Specific and Sequential Role of MyD88 and CARD9 in Chemokine Induction and Innate Defense during Respiratory Fungal Infection. PLOS Pathogens 2015, 11: e1004589. PMID: 25621893, PMCID: PMC4306481, DOI: 10.1371/journal.ppat.1004589.Peer-Reviewed Original ResearchConceptsRespiratory fungal infectionsDistinct signal transduction pathwaysSignal transduction pathwaysNeutrophil recruitmentChemokine inductionGenetic deletionFungal infectionsFungal clearanceCellular compartmentsTransduction pathwaysC-type lectinProtein triggersLung epithelial cellsNeutrophil-dependent host defenseInterleukin-1 receptorReceptor signalsConidial uptakeLung neutrophil recruitmentLung-infiltrating neutrophilsNeutrophil chemokines CXCL1Sequential rolesMyD88-deficient miceHematopoietic compartmentProtein knockout miceMajor cellular source
2014
Mutation of NLRC4 causes a syndrome of enterocolitis and autoinflammation
Romberg N, Al Moussawi K, Nelson-Williams C, Stiegler AL, Loring E, Choi M, Overton J, Meffre E, Khokha MK, Huttner AJ, West B, Podoltsev NA, Boggon TJ, Kazmierczak BI, Lifton RP. Mutation of NLRC4 causes a syndrome of enterocolitis and autoinflammation. Nature Genetics 2014, 46: 1135-1139. PMID: 25217960, PMCID: PMC4177367, DOI: 10.1038/ng.3066.Peer-Reviewed Original Research
2012
The Ability of Virulence Factor Expression by Pseudomonas aeruginosa to Predict Clinical Disease in Hospitalized Patients
Ledizet M, Murray TS, Puttagunta S, Slade MD, Quagliarello VJ, Kazmierczak BI. The Ability of Virulence Factor Expression by Pseudomonas aeruginosa to Predict Clinical Disease in Hospitalized Patients. PLOS ONE 2012, 7: e49578. PMID: 23152923, PMCID: PMC3495863, DOI: 10.1371/journal.pone.0049578.Peer-Reviewed Original ResearchConceptsP. aeruginosa infectionAeruginosa infectionBacterial factorsHospitalized patientsUrinary tractPositive P. aeruginosa culturesP. aeruginosaUrinary tract cathetersP. aeruginosa isolatesLogistic regression modelsPseudomonas aeruginosaProspective cohortDiabetes mellitusSubgroup analysisClinical dataTreatment decisionsClinical diseaseAeruginosa isolatesAnimal modelsPatientsClinical sitesFactor expressionInfectionHost factorsP. aeruginosa culturesThe Carbon Monoxide Releasing Molecule CORM-2 Attenuates Pseudomonas aeruginosa Biofilm Formation
Murray TS, Okegbe C, Gao Y, Kazmierczak BI, Motterlini R, Dietrich LE, Bruscia EM. The Carbon Monoxide Releasing Molecule CORM-2 Attenuates Pseudomonas aeruginosa Biofilm Formation. PLOS ONE 2012, 7: e35499. PMID: 22563385, PMCID: PMC3338523, DOI: 10.1371/journal.pone.0035499.Peer-Reviewed Original ResearchConceptsCORM-2 treatmentP. aeruginosa lung infectionP. aeruginosaAeruginosa lung infectionCORM-2Clinical P. aeruginosaMolecule CORM-2Current antimicrobial agentsChronic infectionLung infectionNew therapiesRelated infectionsNon-mucoid strainsReactive oxygen speciesInfectionNovel therapeutic propertiesTherapeutic propertiesAntimicrobial agentsAdditive effectPseudomonas aeruginosaBiofilm formationOxygen speciesTreatmentAeruginosa
2011
Innate immune responses to Pseudomonas aeruginosa infection
Lavoie EG, Wangdi T, Kazmierczak BI. Innate immune responses to Pseudomonas aeruginosa infection. Microbes And Infection 2011, 13: 1133-1145. PMID: 21839853, PMCID: PMC3221798, DOI: 10.1016/j.micinf.2011.07.011.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsComplement System ProteinsCytokinesDendritic CellsHumansImmunity, InnateInflammasomesLipopolysaccharidesLungLymphocytesMacrophages, AlveolarMiceMice, KnockoutNeutrophilsPneumoniaPseudomonas aeruginosaPseudomonas InfectionsReceptors, Pattern RecognitionSignal TransductionAirway Epithelial MyD88 Restores Control of Pseudomonas aeruginosa Murine Infection via an IL-1–Dependent Pathway
Mijares LA, Wangdi T, Sokol C, Homer R, Medzhitov R, Kazmierczak BI. Airway Epithelial MyD88 Restores Control of Pseudomonas aeruginosa Murine Infection via an IL-1–Dependent Pathway. The Journal Of Immunology 2011, 186: 7080-7088. PMID: 21572023, PMCID: PMC3110630, DOI: 10.4049/jimmunol.1003687.Peer-Reviewed Original ResearchConceptsInnate immune responseImmune responseMyD88-dependent innate immune responsesIL-1-dependent pathwayBone marrow chimeric miceProtective innate immune responseP. aeruginosaNovel transgenic mouse modelVentilator-associated pneumoniaIL-1R signalingTransgenic mouse modelP. aeruginosa infectionEpithelial cell responsesRadio-resistant cellsIntranasal infectionMyD88 expressionMultiple TLR pathwaysMyD88 functionAeruginosa infectionMouse modelTLR pathwayMurine infectionChimeric miceCell responsesInfection
2010
Swarming motility, secretion of type 3 effectors and biofilm formation phenotypes exhibited within a large cohort of Pseudomonas aeruginosa clinical isolates
Murray TS, Ledizet M, Kazmierczak BI. Swarming motility, secretion of type 3 effectors and biofilm formation phenotypes exhibited within a large cohort of Pseudomonas aeruginosa clinical isolates. Journal Of Medical Microbiology 2010, 59: 511-520. PMID: 20093376, PMCID: PMC2855384, DOI: 10.1099/jmm.0.017715-0.Peer-Reviewed Original Research