2015
Alternative Polyadenylation Allows Differential Negative Feedback of Human miRNA miR-579 on Its Host Gene ZFR
Hinske L, Galante P, Limbeck E, Möhnle P, Parmigiani R, Ohno-Machado L, Camargo A, Kreth S. Alternative Polyadenylation Allows Differential Negative Feedback of Human miRNA miR-579 on Its Host Gene ZFR. PLOS ONE 2015, 10: e0121507. PMID: 25799583, PMCID: PMC4370670, DOI: 10.1371/journal.pone.0121507.Peer-Reviewed Original ResearchConceptsHost genesAlternative polyadenylationIntronic miRNAsMiR-579Protein-coding host genesPotential miRNA binding sitesHost gene expressionMiRNA binding sitesIntronic miRNAMiRNA genesNegative feedback loopRNA-seqPolyadenylation signalPolyadenylation sitesBioinformatics analysisCell line modelsGene expressionSilico analysisDifferential targetingPolyadenylationGenesMiRNAsZFRBinding sitesNegative feedback
2014
Prediction of MicroRNA Precursors Using Parsimonious Feature Sets
Stepanowsky P, Levy E, Kim J, Jiang X, Ohno-Machado L. Prediction of MicroRNA Precursors Using Parsimonious Feature Sets. Cancer Informatics 2014, 13s1: cin.s13877. PMID: 25392687, PMCID: PMC4216048, DOI: 10.4137/cin.s13877.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsmiRIAD—integrating microRNA inter- and intragenic data
Hinske L, França G, Torres H, Ohara D, Lopes-Ramos C, Heyn J, Reis L, Ohno-Machado L, Kreth S, Galante P. miRIAD—integrating microRNA inter- and intragenic data. Database 2014, 2014: bau099. PMID: 25288656, PMCID: PMC4186326, DOI: 10.1093/database/bau099.Peer-Reviewed Original ResearchConceptsProtein-coding genesIntragenic miRNAsHost genesGene expressionProtein-protein interaction dataSmall non-coding RNAsHost gene functionHost gene expressionMiRNA binding sitesNon-coding RNAsMajority of miRNAsGene functionGenomic contextFunctional annotationFunctional network analysisTarget mRNAsExpression correlationExonic regionsGenesMiRNAsDifferent tissuesInteraction dataBinding sitesGenomic classificationSilico validation
2013
Differential Expression of miR-145 in Children with Kawasaki Disease
Shimizu C, Kim J, Stepanowsky P, Trinh C, Lau H, Akers J, Chen C, Kanegaye J, Tremoulet A, Ohno-Machado L, Burns J. Differential Expression of miR-145 in Children with Kawasaki Disease. PLOS ONE 2013, 8: e58159. PMID: 23483985, PMCID: PMC3590129, DOI: 10.1371/journal.pone.0058159.Peer-Reviewed Original ResearchMeSH KeywordsArteriesBase SequenceChildChild, PreschoolCluster AnalysisGene Expression RegulationHumansInfantMicroRNAsModels, BiologicalMolecular Sequence DataMucocutaneous Lymph Node SyndromeReal-Time Polymerase Chain ReactionSequence AlignmentSequence Analysis, DNASignal TransductionTransforming Growth Factor betaConceptsTGF-β pathwayGene expressionMiR-145Small non-coding RNAsKawasaki disease pathogenesisExtracellular vesiclesSmall RNA speciesPost-transcriptional levelDiscovery of microRNAsKawasaki diseaseNon-coding RNAsExpression of genesDisease pathogenesisSmall extracellular vesiclesSmall RNAsRNA speciesTarget genesTop pathwaysVascular smooth muscle cellsPathway analysisDifferentiation of neutrophilsDifferential expressionMicroRNAsArterial wallGeneration of myofibroblasts
2012
SurfaceomeDB: a cancer-orientated database for genes encoding cell surface proteins.
de Souza J, Galante P, de Almeida R, da Cunha J, Ohara D, Ohno-Machado L, Old L, de Souza S. SurfaceomeDB: a cancer-orientated database for genes encoding cell surface proteins. Cancer Immunology Research 2012, 12: 15. PMID: 23390370, PMCID: PMC3554024.Peer-Reviewed Original ResearchConceptsCell surface proteinsSurface proteinsProtein-protein interactionsUCSC Genome BrowserImportant data repositoryUser-friendly web interfaceGene annotationProtein domainsGenome browserHuman genesGene expressionGene namesGenesSomatic mutationsProteinExcellent targetAnnotationTherapeutic reagentsWeb interfaceNCBIMutationsExpressionDomainTarget
2011
A Primer on the Current State of Microarray Technologies
Trachtenberg A, Robert J, Abdalla A, Fraser A, He S, Lacy J, Rivas-Morello C, Truong A, Hardiman G, Ohno-Machado L, Liu F, Hovig E, Kuo W. A Primer on the Current State of Microarray Technologies. Methods In Molecular Biology 2011, 802: 3-17. PMID: 22130870, DOI: 10.1007/978-1-61779-400-1_1.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsMicroarray technologyGenome-wide gene expression studiesAvailability of microarraysVariety of genesThousands of genesDNA microarray technologyDisease-specific genesGene expression studiesGene discoveryMolecular functioningExpression studiesGene expressionMolecular geneticsGenesComputer science analysisMicroarrayComplicated networkDisease statesDrug developmentNormal functioningMassive levelsDisease diagnosisGeneticsField of microarraysHigh levels
2007
Comparison of hybridization-based and sequencing-based gene expression technologies on biological replicates
Liu F, Jenssen T, Trimarchi J, Punzo C, Cepko C, Ohno-Machado L, Hovig E, Patrick Kuo W. Comparison of hybridization-based and sequencing-based gene expression technologies on biological replicates. BMC Genomics 2007, 8: 153. PMID: 17555589, PMCID: PMC1899500, DOI: 10.1186/1471-2164-8-153.Peer-Reviewed Original ResearchConceptsDNA microarray platformMicroarray platformSequencing-based approachesSequencing-based technologiesGene expression technologyGene expression profilingTranscriptome profilingBiological replicatesExpression profilingExpression technologyGene expressionDifferential expressionBiomarker discoveryProfilingExpressionMicroarrayImportant tool
2004
Genomic Analysis of Mouse Retinal Development
Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo W, Weber G, Lee K, Fraioli R, Cho S, Yung R, Asch E, Ohno-Machado L, Wong W, Cepko C. Genomic Analysis of Mouse Retinal Development. PLOS Biology 2004, 2: e247. PMID: 15226823, PMCID: PMC439783, DOI: 10.1371/journal.pbio.0020247.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBromodeoxyuridineCell LineageChromosome MappingCluster AnalysisComputational BiologyDatabases, GeneticExpressed Sequence TagsGene Expression RegulationGene Expression Regulation, DevelopmentalGene LibraryGenomeIn Situ HybridizationInterneuronsMiceMitosisMolecular Sequence DataNeurogliaOpen Reading FramesRetinaRNA, MessengerStem CellsTime FactorsConceptsMitotic progenitor cellsRetinal cell typesGene expressionCell typesExpression patternsRetinal developmentDevelopmental gene expression patternsGene expression patternsMajor retinal cell typesOpen reading frameProgenitor cellsMüller gliaPhotoreceptor-enriched genesGene expression profilesMouse retinal developmentMajor cell typesRetinal disease genesGenomic analysisMultiple retinal cell typesChromosomal intervalMolecular atlasMultiple transcriptsReading frameTaxonomic classificationDisease genes