2014
Two Amino Acid Residues Confer Different Binding Affinities of Abelson Family Kinase Src Homology 2 Domains for Phosphorylated Cortactin*
Gifford SM, Liu W, Mader CC, Halo TL, Machida K, Boggon TJ, Koleske AJ. Two Amino Acid Residues Confer Different Binding Affinities of Abelson Family Kinase Src Homology 2 Domains for Phosphorylated Cortactin*. Journal Of Biological Chemistry 2014, 289: 19704-19713. PMID: 24891505, PMCID: PMC4094080, DOI: 10.1074/jbc.m114.556480.Peer-Reviewed Original ResearchConceptsAbl SH2 domainSH2 domainAbl family kinasesFamily kinasesSrc homology 2 domainCell edge protrusionAbl SH2Similar N-terminal sequencesImportant non-redundant rolesWild-type levelsAmino acid sequenceAmino acid positionsN-terminal sequenceNon-redundant roleCell morphogenesisArg substrateCellular functionsSpecific phosphotyrosineCell peripheryAcid sequenceSerine 187Edge protrusionTarget proteinsType levelsDifferent binding affinities
2002
A novel family of anticoagulants from the saliva of Ixodes scapularis
Narasimhan S, Koski RA, Beaulieu B, Anderson JF, Ramamoorthi N, Kantor F, Cappello M, Fikrig E. A novel family of anticoagulants from the saliva of Ixodes scapularis. Insect Molecular Biology 2002, 11: 641-650. PMID: 12421422, DOI: 10.1046/j.1365-2583.2002.00375.x.Peer-Reviewed Original ResearchConceptsN-terminal amino acid sequenceSalivary gland cDNA libraryAmino acid sequenceN-terminal sequenceCDNA libraryAcid sequenceAnticoagulant proteinsIxodes scapularisMolecular approachesTerminal sequenceIntrinsic pathwayEscherichia coliSpecific inhibitorNovel familyProteinSalp14Different functionsTick salivaSequenceBiological activityDose-dependent mannerProtease inhibitorsReversed-phase HPLCParaloguesScapularis
2000
Cellular Mechanisms Regulating Protein Phosphatase-1 A KEY FUNCTIONAL INTERACTION BETWEEN INHIBITOR-2 AND THE TYPE 1 PROTEIN PHOSPHATASE CATALYTIC SUBUNIT*
Frederick D, Huang H, Yang J, Helps N, Cohen P, Nairn A, DePaoli-Roach A, Tatchell K, Connor J, Shenolikar S. Cellular Mechanisms Regulating Protein Phosphatase-1 A KEY FUNCTIONAL INTERACTION BETWEEN INHIBITOR-2 AND THE TYPE 1 PROTEIN PHOSPHATASE CATALYTIC SUBUNIT*. Journal Of Biological Chemistry 2000, 275: 18670-18675. PMID: 10748125, DOI: 10.1074/jbc.m909312199.Peer-Reviewed Original ResearchConceptsPP1 catalytic subunitCatalytic subunitType 1 protein phosphatase catalytic subunitAmino acidsProtein phosphatase catalytic subunitN-terminusProtein serine/threonineN-terminal 35 amino acidsInhibitor 2Phosphatase catalytic subunitTwo-hybrid analysisNovel regulatory interactionsProtein phosphatase 1Serine/threoninePull-down assaysSite-directed mutagenesisN-terminal sequencePP1 mutantsKey functional interactionsPP1 inhibitorPP1 enzymesPP1 inhibitionPhosphatase 1Regulatory interactionsSaccharomyces cerevisiae
1985
Xenopus MHC class II molecules. II. Polymorphism as determined by two-dimensional gel electrophoresis.
Kaufman J, Flajnik M, Du Pasquier L. Xenopus MHC class II molecules. II. Polymorphism as determined by two-dimensional gel electrophoresis. The Journal Of Immunology 1985, 134: 3258-64. PMID: 3980992, DOI: 10.4049/jimmunol.134.5.3258.Peer-Reviewed Original ResearchConceptsTwo-dimensional gel electrophoresisGel electrophoresisRemoval of N-linked glycansXenopus laevisN-terminal sequenceN-linked glycansBiosynthetically labeled cellsClass II antigensPolymorphic classIEF procedureGlycosylated materialAlpha chainPHA-stimulated blood cellsBeta chainInbred strainsEndo FII antigensElectrophoresisMHC typeLaevisOutbred animalsCellsHaplotypesGlycansClones
1972
Oligonucleotide Sequence of Replicase Initiation Site in Qβ RNA
STEITZ J. Oligonucleotide Sequence of Replicase Initiation Site in Qβ RNA. Nature 1972, 236: 71-75. PMID: 4502455, DOI: 10.1038/newbio236071a0.Peer-Reviewed Original ResearchConceptsReplicase geneQβ RNAMajor coat proteinN-terminal sequenceCoat cistronTermination signalReplicase cistronNucleotide sequenceCoat proteinRNA genomeInitiator codonInitiation siteQβ replicaseGenesProteinBacteriophage QβCistronUGA suppressorRNAVirus particlesSequenceOligonucleotide sequencesProtein7GenomeReplicase
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