2021
Cryo-EM structures of Escherichia coli cytochrome bo3 reveal bound phospholipids and ubiquinone-8 in a dynamic substrate binding site
Li J, Han L, Vallese F, Ding Z, Choi SK, Hong S, Luo Y, Liu B, Chan CK, Tajkhorshid E, Zhu J, Clarke O, Zhang K, Gennis R. Cryo-EM structures of Escherichia coli cytochrome bo3 reveal bound phospholipids and ubiquinone-8 in a dynamic substrate binding site. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2106750118. PMID: 34417297, PMCID: PMC8403832, DOI: 10.1073/pnas.2106750118.Peer-Reviewed Original ResearchConceptsHydrogen bondsMembrane scaffold protein (MSP) nanodiscsSide chainsMetal redox centerCryo-EM structureInternal water moleculesEscherichia coli cytochrome bo3Facilitate proton transferImidazole side chainIsoprene side chainAccess of waterCryogenic electron microscopyRedox centersWater moleculesProton transferSubunit IAqueous phaseConformation 1Respiratory cytochromesRelated cytochromesHydrophobic grooveUbiquinol oxidaseQuinol oxidaseCytochrome bo3Computational study
2001
Effects of tail‐like substituents on the binding of competitive inhibitors to the QB site of photosystem II
Reifler M, Szalai V, Peterson C, Brudvig G. Effects of tail‐like substituents on the binding of competitive inhibitors to the QB site of photosystem II. Journal Of Molecular Recognition 2001, 14: 157-165. PMID: 11391786, DOI: 10.1002/jmr.529.Peer-Reviewed Original ResearchConceptsQB siteAqueous phasePhotosystem IIBulk aqueous phaseCritical micelle concentrationTriazine-type herbicidesQuinone-binding sitePhenylurea moietyEffect of chargeAlkyl chainsHydrophobic tailSolvent conditionsMicelle concentrationPara positionPhenylurea compoundsS-triazineAlkylamino groupSubstituentsSmall libraryHydrophobic linkerMolecular rulerHydrophilic domainsNonspecific bindingChargeCompetitive inhibitor
2000
Partitioning of Ethoxylated Nonionic Surfactants in Water/NAPL Systems: Effects of Surfactant and NAPL Properties
Cowell M, Kibbey T, Zimmerman J, Hayes K. Partitioning of Ethoxylated Nonionic Surfactants in Water/NAPL Systems: Effects of Surfactant and NAPL Properties. Environmental Science And Technology 2000, 34: 1583-1588. DOI: 10.1021/es9908826.Peer-Reviewed Original ResearchWater interfacial tensionNonionic surfactantsEffect of surfactantsSurfactant hydrophobicityInterfacial tensionNonaqueous phase liquidsSurfactant critical micelle concentrationEthoxylated nonionic surfactantsSurfactant remediationHigh surfactant concentrationsCritical micelle concentrationDifferent chemical structuresAmphiphilic moleculesChemical structureAqueous phaseOrganic phaseMicelle concentrationNAPL propertiesSurfactant concentrationNAPL contaminantsNonaqueous phaseSurfactantsSurfactant partitioningFinite solubilityPolydispersity
1998
Synthesis and characterization of an internal emission standard and applications to fluorescence studies of photosystem II
Schweitzer R, Brudvig G. Synthesis and characterization of an internal emission standard and applications to fluorescence studies of photosystem II. Biopolymers 1998, 2: 167-171. DOI: 10.1002/(sici)1520-6343(1996)2:3<167::aid-bspy3>3.0.co;2-5.Peer-Reviewed Original ResearchChelate complexesFluorescence intensityFluorescence measurementsDiethylenetriaminepentaacetic acid derivativeTerbium luminescenceAqueous phaseTerbium emissionAcid derivativesPyrimidine ringComplexesPhotosystem II functionLow temperaturePhotosynthetic organismsLow absorptionCryogenic temperaturesLanthanidesDifferent samplesLigandsSynthesisLuminescenceTerbiumTemperatureDerivativesPurificationEmission standards
1989
Molecular basis of the heat denaturation of photosystem II.
Thompson L, Blaylock R, Sturtevant J, Brudvig G. Molecular basis of the heat denaturation of photosystem II. Biochemistry 1989, 28: 6686-95. PMID: 2675973, DOI: 10.1021/bi00442a023.Peer-Reviewed Original ResearchConceptsDifferential scanning calorimetryPSII complexesMembrane proteinsMn complexesAntenna proteinsThermal gel analysisElectron paramagnetic resonance measurementsGel analysisMembrane protein complexesMajor membrane proteinParamagnetic resonance measurementsElectron transport chainRelease of MnWater oxidationA2 peakHydroxide ionPSII membranesProtein complexesAqueous phaseComponent essentialSolubility propertiesDSC studiesMolecular basisEPR experimentsPSII core
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