2020
A CRISPR Interference Platform for Selective Downregulation of Gene Expression in Borrelia burgdorferi
Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C. A CRISPR Interference Platform for Selective Downregulation of Gene Expression in Borrelia burgdorferi. Applied And Environmental Microbiology 2020, 87: e02519-20. PMID: 33257311, PMCID: PMC7851697, DOI: 10.1128/aem.02519-20.Peer-Reviewed Original ResearchCRISPR interference platformDifferent antibiotic resistance markersGene functionAntibiotic resistance markersGene expressionHomologous recombination-based methodsCell morphogenesis genesCell wall elongationNative expression levelsBasic cellular processesRecombination-based methodGene function studiesCryo-electron tomographyFuture genetic studiesMorphogenesis genesResistance markersRepression efficiencyCell straighteningGenetic toolsCellular processesWall elongationPeriplasmic flagellaCell filamentationCell divisionCellular motility
2017
Sox17 drives functional engraftment of endothelium converted from non-vascular cells
Schachterle W, Badwe C, Palikuqi B, Kunar B, Ginsberg M, Lis R, Yokoyama M, Elemento O, Scandura J, Rafii S. Sox17 drives functional engraftment of endothelium converted from non-vascular cells. Nature Communications 2017, 8: 13963. PMID: 28091527, PMCID: PMC5260855, DOI: 10.1038/ncomms13963.Peer-Reviewed Original ResearchConceptsEndothelial cellsMorphogenesis genesETS transcription factorsVascular endothelial cellsTreat vasculopathyAmniotic cellsGenomic targetsConsensus sitesTransplantation strategiesInjured vesselsParacrine factorsTranscription factorsNon-vascular cellsSOX17 expressionVascular functionFunctional engraftmentTransplantation studiesGene expressionPromote tissue regenerationSOX17Support metabolismEnforced expressionEC-like cellsEC gene expressionTransplantation
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply