2011
Ammonia Channeling in Plasmodium falciparum GMP Synthetase: Investigation by NMR Spectroscopy and Biochemical Assays
Bhat JY, Venkatachala R, Singh K, Gupta K, Sarma SP, Balaram H. Ammonia Channeling in Plasmodium falciparum GMP Synthetase: Investigation by NMR Spectroscopy and Biochemical Assays. Biochemistry 2011, 50: 3346-3356. PMID: 21413787, DOI: 10.1021/bi1017057.Peer-Reviewed Original ResearchConceptsGMP synthetasePlasmodium falciparum GMP synthetaseBiochemical assaysPurine biosynthetic pathwayAmmonia channelingGlutamine amidotransferasesGlutaminase domainMolecular tunnelBiosynthetic pathwayCatalytic residuesGlutaminase siteSeparate active sitesExternal mediumThioester intermediateExternal ammoniaCys-102NMR spectroscopyConcentration of glutamineSynthetaseUncompetitive inhibitorGlutamineActive siteSynthetase siteProton NMR spectroscopyAssays
2006
A Molecular Tunnel Required for Cooperation of an Asparaginase and a Glu‐tRNAGln Kinase in Gln‐tRNA Formation
Sheppard K, Feng L, Oshikane H, Nakamura Y, Fukai S, Nureki O, Söll D. A Molecular Tunnel Required for Cooperation of an Asparaginase and a Glu‐tRNAGln Kinase in Gln‐tRNA Formation. The FASEB Journal 2006, 20: a503-a503. DOI: 10.1096/fasebj.20.4.a503-a.Peer-Reviewed Original ResearchGlu-tRNAGlnMolecular tunnelMost prokaryotesCo-crystal structurePresence of ATPGln-tRNAGlnSequence similarityEvolutionary linkHeterodimeric enzymeStructural insightsGatDEGatDEnzyme showEnzymatic analysisKinaseAmide donorCrystal structureActive siteATPGlnGluProkaryotesArchaeaTransamidationTight coupling
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