2025
Unbiased mapping of cereblon neosubstrate landscape by high-throughput proteomics
Steger M, Nishiguchi G, Wu Q, Schwalb B, Shashikadze B, McGowan K, Actis M, Aggarwal A, Shi Z, Price J, Mayasundari A, Yang L, Bednarz A, Machata S, Graef T, Bartoschek D, Demichev V, Ohmayer U, Yang J, Daub H, Rankovic Z. Unbiased mapping of cereblon neosubstrate landscape by high-throughput proteomics. Nature Communications 2025, 16: 7773. PMID: 40835825, PMCID: PMC12368047, DOI: 10.1038/s41467-025-62829-0.Peer-Reviewed Original ResearchConceptsMolecular glue degradersHigh-throughput proteomic platformsData-independent acquisition mass spectrometryDIA-MSMass spectrometryHigh-throughput proteomicsSmall moleculesDrug discoveryGlue degradersRelationship analysisLabel-freeScreening approachTarget proteinsPhenylNovel degradersImideDegradation of target proteinsProteomics platformUbiquitin-proteasome systemNeosubstratesLigandDegradationSpectrometryMoleculesProteome-wide screening approach
2015
Label-Free Biosensor Imaging on Photonic Crystal Surfaces
Zhuo Y, Cunningham B. Label-Free Biosensor Imaging on Photonic Crystal Surfaces. Sensors 2015, 15: 21613-21635. PMID: 26343684, PMCID: PMC4610529, DOI: 10.3390/s150921613.Peer-Reviewed Original ResearchConceptsPhotonic crystal surfaceCrystal surfaceImaging detection instrumentMetal plasmonic nanoparticlesDielectric nanoparticlesPhotobleachable fluorescent dyesOptical scatteringDielectric permittivityNanostructure designSensing resolutionOperating principleSubstrate surfaceCytotoxic stainsOptical microscopyPlasmonic nanoparticlesBiomaterial interactionsMatrix surfaceBiomolecular layerDetection instrumentKinetic monitoringPhotonsLabel-freeNanoparticlesSurfaceNanoparticle contrast agents
2013
Photonic crystal enhanced microscopy for imaging of live cell adhesion
Chen W, Long K, Lu M, Chaudhery V, Yu H, Choi J, Polans J, Zhuo Y, Harley B, Cunningham B. Photonic crystal enhanced microscopy for imaging of live cell adhesion. Analyst 2013, 138: 5886-5894. PMID: 23971078, DOI: 10.1039/c3an01541f.Peer-Reviewed Original ResearchConceptsTime-resolved imagingPhotonic crystalsRelevant time-scalesTime-scalesDrug-induced apoptosisSubmicron resolutionSpatial resolutionMorphological signaturesStem cell differentiationCell-surface interactionsFilopodial extensionsPhotonsCell adhesionCell differentiationDynamic informationCell attachmentLiving cell adhesionMonitor cell behaviorCell behaviorBiological phenomenaResolutionApoptosisEvolutionChemotaxisLabel-free
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