2020
Phosphoinositide 3′-Kinase γ Facilitates Polyomavirus Infection
Clark P, Gee GV, Albright BS, Assetta B, Han Y, Atwood WJ, DiMaio D. Phosphoinositide 3′-Kinase γ Facilitates Polyomavirus Infection. Viruses 2020, 12: 1190. PMID: 33092168, PMCID: PMC7589550, DOI: 10.3390/v12101190.Peer-Reviewed Original ResearchConceptsCRISPR/Cas9-mediated knockoutGenetic knockdown approachesCas9-mediated knockoutDNA tumor virusesProgressive multifocal leukoencephalopathyInfection defectPolyomavirus infectionCellular proteinsKnockdown approachSVG-A cellsPolyomavirus entryMolecular eventsVirus internalizationTumor virusPI3Kγ pathwayPIK3R5PI3KγGlial cellsKnockoutMultifocal leukoencephalopathyImmunosuppressed peopleCellsHuman polyomavirusesJC polyomavirusPolyomavirus
2018
Striatin-1 is a B subunit of protein phosphatase PP2A that regulates dendritic arborization and spine development in striatal neurons
Li D, Musante V, Zhou W, Picciotto MR, Nairn AC. Striatin-1 is a B subunit of protein phosphatase PP2A that regulates dendritic arborization and spine development in striatal neurons. Journal Of Biological Chemistry 2018, 293: 11179-11194. PMID: 29802198, PMCID: PMC6052221, DOI: 10.1074/jbc.ra117.001519.Peer-Reviewed Original ResearchConceptsSerine/threonine phosphatase PP2AStriatin-interacting phosphataseRNA knockdown approachB subunitSTRIPAK complexPhosphatase PP2AProtein phosphataseMultiprotein complexesKnockdown approachStriatin familyMutant constructsStriatal neuronal culturesPP2ANeuronal developmentPrimary striatal neuronal culturesDendritic phenotypeKnockdown modelSynapse formationSubunitsSpine developmentSelective roleReduced expressionNeuron maturationNeuronal culturesStriatal neurons
2012
Analysis of lipolysis underlying lactation in the tsetse fly, Glossina morsitans
Attardo GM, Benoit JB, Michalkova V, Yang G, Roller L, Bohova J, Takáč P, Aksoy S. Analysis of lipolysis underlying lactation in the tsetse fly, Glossina morsitans. Insect Biochemistry And Molecular Biology 2012, 42: 360-370. PMID: 22509523, PMCID: PMC3561780, DOI: 10.1016/j.ibmb.2012.01.007.Peer-Reviewed Original ResearchConceptsSimultaneous knockdownLipid reservesLarval productionAdipokinetic hormoneEarly larval developmentLate larval instarsTsetse fliesLipid metabolic pathwaysBrummer lipaseLarval nourishmentViviparous reproductionLipid metabolismIndividual knockdownTsetse fecundityKnockdown approachFemale lifespanHormone-receptor systemLarval developmentTranscript levelsAKHRLarval instarsMetabolic pathwaysStarvation experimentsKnockdownOocyte developmentDifferent effects of Sec61α, Sec62 and Sec63 depletion on transport of polypeptides into the endoplasmic reticulum of mammalian cells
Lang S, Benedix J, Fedeles SV, Schorr S, Schirra C, Schäuble N, Jalal C, Greiner M, Haßdenteufel S, Tatzelt J, Kreutzer B, Edelmann L, Krause E, Rettig J, Somlo S, Zimmermann R, Dudek J. Different effects of Sec61α, Sec62 and Sec63 depletion on transport of polypeptides into the endoplasmic reticulum of mammalian cells. Journal Of Cell Science 2012, 125: 1958-1969. PMID: 22375059, PMCID: PMC4074215, DOI: 10.1242/jcs.096727.Peer-Reviewed Original ResearchConceptsPost-translational transportTail-anchored proteinsSEC61A1 geneEndoplasmic reticulumTransport of polypeptidesCo-translational transportSemi-permeabilized cellsPrecursor proteinSEC62 geneSec61 channelPresecretory proteinsMembrane integrationProtein transportMammalian cellsKnockdown approachHuman cellsGenesHeLa cellsProteinPolypeptideReticulumCellsSec63pSec61αSec63
2005
Gene Therapy for Autosomal Dominant Disorders of Keratin
Lewin AS, Glazer PM, Milstone LM. Gene Therapy for Autosomal Dominant Disorders of Keratin. Journal Of Investigative Dermatology Symposium Proceedings 2005, 10: 47-61. PMID: 16250209, DOI: 10.1111/j.1087-0024.2005.10207.x.Commentaries, Editorials and LettersMeSH KeywordsAnimalsDarier DiseaseDependovirusEctodermal DysplasiaEpidermolysis Bullosa SimplexGene SilencingGene TargetingGenes, DominantGenetic TherapyGenetic VectorsHumansKeratinsKeratoderma, PalmoplantarMiceMutationNails, MalformedOligonucleotides, AntisenseRNA InterferenceRNA, CatalyticRNA, Small InterferingConceptsRNA knockdown approachGene correctionGene therapyViral vectorsEpidermal skin cellsKeratin diseasesKnockdown approachRNA interferenceGene expressionEpidermolysis bullosa simplexToxic proteinsDominant mutationsGenetic diseasesGenetic therapiesKeratin filamentsEpidermal diseasesGenetic defectsTissue cultureSelective inhibitorSkin cellsAttractive alternativeAutosomal dominant disorderRecent innovationsDominant disorderNear future
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