2022
Carnegie in 4D? Stem-cell-based models of human embryo development
Sozen B, Conkar D, Veenvliet J. Carnegie in 4D? Stem-cell-based models of human embryo development. Seminars In Cell And Developmental Biology 2022, 131: 44-57. PMID: 35701286, DOI: 10.1016/j.semcdb.2022.05.023.Peer-Reviewed Original ResearchConceptsEmbryo developmentMouse in vitro modelsHuman embryo developmentHuman pluripotent stem cellsPluripotent stem cellsModels of embryo developmentHuman developmentInvestigation of embryogenesisHuman embryosStem cellsPrimate embryosIn vitro approachesIn vitroMammalian embryogenesisIn vitro platformMammalian developmentMiceEmbryosNatural embryoGastrulation stageCellsTranscriptional regulationOver-relianceFetusesUtero
2019
Genetic and Biochemical Analyses of Yeast ESCRT
Banjade S, Tang S, Emr S. Genetic and Biochemical Analyses of Yeast ESCRT. Methods In Molecular Biology 2019, 1998: 105-116. PMID: 31250297, DOI: 10.1007/978-1-4939-9492-2_8.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid Transport Systems, BasicEndocytosisEndosomal Sorting Complexes Required for TransportGreen Fluorescent ProteinsMembrane LipidsMembranes, ArtificialMicroscopy, ElectronMicroscopy, FluorescenceMultivesicular BodiesMutationProtein MultimerizationRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsVacuolesConceptsMultivesicular bodiesBudding yeast Saccharomyces cerevisiaeBiogenesis of multivesicular bodiesUbiquitinated membrane proteinsMembrane trafficking pathwaysYeast Saccharomyces cerevisiaeESCRT-III polymerizationFormation of multivesicular bodiesIdeal model organismESCRT functionESCRT-IIISaccharomyces cerevisiaeTrafficking pathwaysModel organismsBiochemical approachesEndocytosis assayMembrane proteinsESCRTIn vitro approachesBiochemical analysisModel cargoBiogenesisEndosomesOrganellesTrafficking
2015
Using SRM-MS to quantify nuclear protein abundance differences between adipose tissue depots of insulin-resistant mice
Ota A, Kovary K, Wu O, Ahrends R, Shen W, Costa M, Feldman B, Kraemer F, Teruel M. Using SRM-MS to quantify nuclear protein abundance differences between adipose tissue depots of insulin-resistant mice. Journal Of Lipid Research 2015, 56: 1068-1078. PMID: 25840986, PMCID: PMC4409283, DOI: 10.1194/jlr.d056317.Peer-Reviewed Original ResearchConceptsWhite adipose tissueStromal vascular cellsInsulin-resistant mouse modelProtein abundance differencesProtein abundance profilesPrimary adipose tissueCell culture cellsDifferentially expressed proteinsWhite adipose tissue depotsVisceral white adipose tissueInsulin-resistant miceProtein expression patternsNuclear proteinsProteomic approachProtein abundanceCell culture modelExpression patternsAdipocyte cell culturesIn vitro approachesInsulin resistanceCultured cellsAbundance profilesAdipose tissueImpaired differentiationProtein
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