2019
Machine Learning Based Real‐Time Image‐Guided Cell Sorting and Classification
Gu Y, Zhang A, Han Y, Li J, Chen C, Lo Y. Machine Learning Based Real‐Time Image‐Guided Cell Sorting and Classification. Cytometry Part A 2019, 95: 499-509. PMID: 30958640, PMCID: PMC6520141, DOI: 10.1002/cyto.a.23764.Peer-Reviewed Original ResearchConceptsCell sortingCell-to-cell variationNext generation sequencingFluorescence Activated Cell SortingFluorescence-activated cell sortingGenomic-based assaysGenetic informationGeneration sequencingNuclear localizationLocalization of glucocorticoid receptorsPathology of biological systemsCell membraneGlucocorticoid receptorCellsIsolated cellsBiological systemsSortingFlow cytometerCell classificationInformation contentDNASequenceOptical scatteringAssayPhysiology
2016
Computational cell analysis for label-free detection of cell properties in a microfluidic laminar flow
Zhang A, Gu Y, Han Y, Mei Z, Chiu Y, Geng L, Cho S, Lo Y. Computational cell analysis for label-free detection of cell properties in a microfluidic laminar flow. Analyst 2016, 141: 4142-4150. PMID: 27163941, PMCID: PMC4914432, DOI: 10.1039/c6an00295a.Peer-Reviewed Original ResearchConceptsCell typesCell analysis techniquesPhysical properties of cellsDrug-treated cellsFlow cytometerLaminar flowCell stiffnessMachine learning techniquesMolecular markersProperties of cellsHuman cellsSpatial coding techniqueTreated cellsLabel-free methodCancer cellsConventional flow cytometersUntreated cellsMicrofluidic laminar flowLearning techniquesPhysical interactionCell membraneCell mixturesCell sizeCell analysisCells
2014
Assessment of Phagocytic Activity of Cultured Macrophages Using Fluorescence Microscopy and Flow Cytometry
Sharma L, Wu W, Dholakiya SL, Gorasiya S, Wu J, Sitapara R, Patel V, Wang M, Zur M, Reddy S, Siegelaub N, Bamba K, Barile FA, Mantell LL. Assessment of Phagocytic Activity of Cultured Macrophages Using Fluorescence Microscopy and Flow Cytometry. Methods In Molecular Biology 2014, 1172: 137-145. PMID: 24908301, DOI: 10.1007/978-1-4939-0928-5_12.ChaptersMeSH KeywordsAnimalsCell LineFlow CytometryFluorescein-5-isothiocyanateFluorescent DyesGene ExpressionGranulocyte-Macrophage Colony-Stimulating FactorHMGB1 ProteinInterleukin-1betaMacrophagesMiceMicroscopy, FluorescenceMicrospheresPhagocytosisTransforming Growth Factor beta1Tumor Necrosis Factor-alphaConceptsCultured macrophagesHigh mobility group box 1Individual cellsInnate immune systemFluorescence microscopyNumerous diseasesPhagocytic processFluorescence microscopePhagocytic functionPhagocytic activityCell debrisPhagocytosisFlow cytometryMobility group box 1PhagocytesGroup box 1GM-CSFMacrophagesImmune systemTGF-β1Different cytokinesFlow cytometerIL-1βEngulfmentHomeostasis
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