2007
Hydrogen/deuterium exchange-mass spectrometry: a powerful tool for probing protein structure, dynamics and interactions.
Tsutsui Y, Wintrode P. Hydrogen/deuterium exchange-mass spectrometry: a powerful tool for probing protein structure, dynamics and interactions. Current Medicinal Chemistry 2007, 14: 2344-58. PMID: 17896983, DOI: 10.2174/092986707781745596.Peer-Reviewed Original ResearchConceptsProtein assembliesMolecular basisProtein structureNuclear magnetic resonance spectroscopyHydrogen/deuterium exchangeLarge protein assembliesD exchange processX-ray crystallographyDrugs/inhibitorsDynamics of proteinsBackbone amide hydrogensExchange processMagnetic resonance spectroscopyLocal structural environmentPatho-physiological processesViral capsid structureAmide hydrogensHXMSDeuterium exchangeMass spectrometrySmall sample requirementDrug designHigh-quality crystalsResonance spectroscopyProtein conformation
2003
Online Hydrogen-Deuterium Exchange and a Tandem-Quadrupole Time-of-Flight Mass Spectrometer Coupled with Liquid Chromatography for Metabolite Identification in Drug Metabolism*
Nassar A. Online Hydrogen-Deuterium Exchange and a Tandem-Quadrupole Time-of-Flight Mass Spectrometer Coupled with Liquid Chromatography for Metabolite Identification in Drug Metabolism*. Journal Of Chromatographic Science 2003, 41: 398-404. PMID: 14558931, DOI: 10.1093/chromsci/41.8.398.Peer-Reviewed Original ResearchConceptsMetabolite identificationMobile phaseHydrogen-deuterium (H-D) exchangeH-D exchange processTime-of-flight mass spectrometerDemethylation of methoxy groupsHydroxylation of methyl groupsH-D exchangeHydrogen-deuterium exchangeIdentification of metabolitesLiquid chromatographyMethoxy groupEster groupsMethyl groupMetabolite elucidationMass spectrometerHuman hepatic microsomesD(2)ODrug discoveryDehydrogenationExchange processRetention timeEquilibration timeOxidationNo significant difference
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