Massively parallel enrichment of low-frequency alleles enables duplex sequencing at low depth
Gydush G, Nguyen E, Bae JH, Blewett T, Rhoades J, Reed SC, Shea D, Xiong K, Liu R, Yu F, Leong KW, Choudhury AD, Stover DG, Tolaney SM, Krop IE, Christopher Love J, Parsons HA, Mike Makrigiorgos G, Golub TR, Adalsteinsson VA. Massively parallel enrichment of low-frequency alleles enables duplex sequencing at low depth. Nature Biomedical Engineering 2022, 6: 257-266. PMID: 35301450, PMCID: PMC9089460, DOI: 10.1038/s41551-022-00855-9.Peer-Reviewed Original ResearchConceptsLow-frequency mutationsDuplex SequencingNumber of lociLow-frequency allelesWhole-genome sequencingHuman cell linesSingle nucleotide polymorphismsGenomic DNAWhole-exome sequencingMutation enrichmentParallel enrichmentBreast tumor samplesSequencingLociMutationsDistinct mutationsCell linesDNADetection of mutationsReadsChapter 2 Overview of liquid biopsy
Aghamir S, Khatami F, Yarandi V, Rezaeian A, Fiorentino M. Chapter 2 Overview of liquid biopsy. 2022, 5-30. DOI: 10.1016/b978-0-323-99884-0.00004-5.Peer-Reviewed Original ResearchLiquid biopsy analysisTumor-derived cellsLiquid biopsyBiopsy analysisCell-free circulating tumor DNAApplication of liquid biopsyLiquid biopsy componentsCharacterization of CTCsPredictive of responseDetection of mutationsMinimally invasive biomarkersTumor characteristicsLiquid biopsy dataUrogenital cancersTumor DNADroplet digital PCRBiopsy dataTherapeutic decisionsBiopsyNext-generation sequencingTumorInvasive biomarkersMedical strategiesCancerPersonalized medicine
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