Perturb-tracing enables high-content screening of multi-scale 3D genome regulators
Cheng Y, Hu M, Yang B, Jensen T, Zhang Y, Yang T, Yu R, Ma Z, Radda J, Jin S, Zang C, Wang S. Perturb-tracing enables high-content screening of multi-scale 3D genome regulators. Nature Methods 2025, 22: 950-961. PMID: 40211002, PMCID: PMC12074983, DOI: 10.1038/s41592-025-02652-z.Peer-Reviewed Original ResearchConceptsLoss-of-function screensPooled CRISPR screensIdentified new regulatorsGenome organizationChromatin domainsGenome regulationChromatin organizationChromosome territoriesChromatin tracingChromatin foldingChromatin topologyCRISPR screensHigh-content screeningChromatin compactionNuclear architectureLoop extrusionChromatinGenomic mechanismsHigh-content screening platformHuman cellsCompartmentalization mechanismFunctional linkTopological regulationGenomic effectsScreening platform
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