2025
Nasopharyngeal Carriage, Antimicrobial Resistance, and Serotype Distribution of Streptococcus pneumoniae in Children Under Five in Lebanon: Baseline Data Prior to PCV13 Introduction
Rafei R, Zaylaa M, Diab M, Kassem I, Omari K, Halimeh F, Moujaber G, Achour A, Ismail B, Mallat H, Hamze M, Dabboussi F, Osman M. Nasopharyngeal Carriage, Antimicrobial Resistance, and Serotype Distribution of Streptococcus pneumoniae in Children Under Five in Lebanon: Baseline Data Prior to PCV13 Introduction. Antibiotics 2025, 14: 168. PMID: 40001412, PMCID: PMC11851980, DOI: 10.3390/antibiotics14020168.Peer-Reviewed Original ResearchPneumococcal conjugate vaccineCarriage of pneumococciNasopharyngeal carriageSerotype distribution of Streptococcus pneumoniaePCV13 vaccination coverageAntimicrobial susceptibility testingAntimicrobial-resistance profilesLevels of antibiotic resistanceInvasive pneumococcal diseaseStreptococcus pneumoniae</i>Pneumococcal conjugate vaccine vaccinationNew vaccine developmentPneumococci isolatesPCV13 introductionTrimethoprim-sulfamethoxazoleCarriage serotypesVaccine serotypesSerotype distributionSusceptibility testingAntimicrobial-resistantPositive culturesInvasive diseaseMultidrug resistancePneumococcal diseaseSource of transmission
2024
A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva
Peno C, Lin T, Hislop M, Yolda-Carr D, Farjado K, York A, Pitzer V, Weinberger D, Bei A, Allicock O, Wyllie A. A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva. Microbiology Spectrum 2024, 12: e00591-24. PMID: 39028185, PMCID: PMC11370248, DOI: 10.1128/spectrum.00591-24.Peer-Reviewed Original ResearchDetection of pneumococciDetection of pneumococcal carriagePneumococcal carriageCarriage surveillanceLow-resource settingsChildren attending childcare centersCarriage of pneumococciDNA extractionSaliva samplesMolecular methodsCultural enrichmentImprove surveillance effortsQPCR-based protocolPneumococcal vaccineExtraction-free methodMolecular detectionNucleic acid extractionVaccination strategiesPneumococciCulture-enrichment methodExtraction-free protocolPurified DNASalivaPaired samplesCarriage
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