2025
Online Alkaline-pH Reversed-Phase Nanoelectrospray-Tandem Mass Spectrometry Complements Traditional Phosphoproteomic Analysis via Influencing Charge State Distribution of Phosphopeptides
Wang Y, Gao J, Xie W, Tang M, Chen X, Chen L, Chen H, Yang Z, Gao Q, Liu Y, Zhou H. Online Alkaline-pH Reversed-Phase Nanoelectrospray-Tandem Mass Spectrometry Complements Traditional Phosphoproteomic Analysis via Influencing Charge State Distribution of Phosphopeptides. Journal Of Proteome Research 2025, 24: 2443-2453. PMID: 40205994, DOI: 10.1021/acs.jproteome.4c01091.Peer-Reviewed Original ResearchConceptsNanoelectrospray tandem mass spectrometryPhosphopeptide enrichment techniquesNanoelectrospray ionizationTandem MSMass spectrometerC18 columnNormal adjacent tissuesMass spectrometryPairs of hepatocellular carcinomaPhosphorylated peptidesLiquid chromatography fractionationPhosphopeptidesHigh pHActual polarizationBiological functionsPhosphoproteomic analysisChromatography fractionsIonizationSpectrometryLow pHEnrichment techniquesHepatocellular carcinomaSpectrometerAdjacent tissuesGlobal phosphorylation
2024
O-Glycoproteomics: Methods, Challenges, and New Opportunities
Riley N, Malaker S. O-Glycoproteomics: Methods, Challenges, and New Opportunities. 2024, 118-162. DOI: 10.1039/9781839166433-00118.Peer-Reviewed Original ResearchElectron transfer dissociationMass spectrometryBeam-type collision-induced dissociationSupplemental collisional activationCollision-induced dissociationSolid-phase extractionCollisional activationTransfer dissociationGlycopeptide analysisTandem MSO-glycoproteomeDissociationO-glycoproteinsSite-specific localizationGlycopeptidesEThcDO-glycansSpectrometryGlycoproteomicsSpectraElectron
2013
Liquid chromatography quadrupole time-of-flight mass spectrometry characterization of metabolites guided by the METLIN database
Zhu ZJ, Schultz AW, Wang J, Johnson CH, Yannone SM, Patti GJ, Siuzdak G. Liquid chromatography quadrupole time-of-flight mass spectrometry characterization of metabolites guided by the METLIN database. Nature Protocols 2013, 8: 451-460. PMID: 23391889, PMCID: PMC3666335, DOI: 10.1038/nprot.2013.004.Peer-Reviewed Original ResearchConceptsLiquid chromatography-quadrupole timeProfiling experimentsMETLIN metabolite databaseMore populationsSeven-step protocolFlight mass spectrometry characterizationMost biological samplesFlight mass spectrometryMass spectrometry characterizationTandem MSMetabolomic featuresQuadrupole timeComprehensive platformUntargeted metabolomicsMass spectrometryMS dataMETLIN databaseLC-Q-TOFMetabolitesMetabolite databasesBiological samplesThousands of peaks
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