2020
DNA Strand Exchange to Monitor Human RAD51-Mediated Strand Invasion and Pairing
Lahiri S, Jensen RB. DNA Strand Exchange to Monitor Human RAD51-Mediated Strand Invasion and Pairing. Methods In Molecular Biology 2020, 2153: 101-113. PMID: 32840775, PMCID: PMC10434838, DOI: 10.1007/978-1-0716-0644-5_8.Peer-Reviewed Original ResearchConceptsDNA double-strand breaksDNA strand exchangeStrand invasionSingle-strand DNA gapsStrand exchangeDNA DSBsHuman Rad51 proteinDNA strand exchange reactionDouble-strand breaksHomologous recombination pathwayHigh-fidelity repairStrand exchange reactionGenomic integrityRad51 proteinReplication forksDNA substratesHomology searchDNA gapsGenetic informationMitotic cellsRecombination pathwayDNA moleculesProteinInvasionMeiosis
2019
Chromatin‐bound cGAS is an inhibitor of DNA repair and hence accelerates genome destabilization and cell death
Jiang H, Xue X, Panda S, Kawale A, Hooy RM, Liang F, Sohn J, Sung P, Gekara NO. Chromatin‐bound cGAS is an inhibitor of DNA repair and hence accelerates genome destabilization and cell death. The EMBO Journal 2019, 38: embj2019102718. PMID: 31544964, PMCID: PMC6826206, DOI: 10.15252/embj.2019102718.Peer-Reviewed Original ResearchConceptsCyclic GMP-AMP synthaseHomologous recombinationDNA repairGenome destabilizationCell deathChromatin-binding proteinsGMP-AMP synthaseSsDNA filamentsGenome integrityGenomic stressInnate immune sensorsHR repairChromosomal rearrangementsStrand invasionRegulatory mechanismsUnknown roleCytosolic innate immune sensorsCell survivalTemplate dsDNAImmune sensorsBone marrow cellsMicronuclei generationOutcome of infectionMarrow cellsInnate immune activation
2016
A multi-step model for facilitated unwinding of the yeast U4/U6 RNA duplex
Rodgers M, Didychuk A, Butcher S, Brow D, Hoskins A. A multi-step model for facilitated unwinding of the yeast U4/U6 RNA duplex. Nucleic Acids Research 2016, 44: 10912-10928. PMID: 27484481, PMCID: PMC5159527, DOI: 10.1093/nar/gkw686.Peer-Reviewed Original ResearchConceptsSmall nuclear RNATri-snRNPU4/U6 unwindingU6 small nuclear RNAImpairs yeast growthSpliceosome active siteU4/U6 di-snRNPSmall nuclear ribonucleoproteinTri-snRNP assemblySpliceosome formationStrand invasionDi-snRNPYeast growthNuclear ribonucleoproteinNuclear RNARNA complexResonance energy transferSpliceosomeRNA duplexesForster resonance energy transferConformational rearrangementsDNA oligonucleotidesStem IIUnwindingU4/U6
2014
Induction of homologous recombination between sequence repeats by the activation induced cytidine deaminase (AID) protein
Buerstedde JM, Lowndes N, Schatz DG. Induction of homologous recombination between sequence repeats by the activation induced cytidine deaminase (AID) protein. ELife 2014, 3: e03110. PMID: 25006166, PMCID: PMC4080448, DOI: 10.7554/elife.03110.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceB-LymphocytesCell LineChickensCrossing Over, GeneticCytidine DeaminaseGene ConversionGenes, ReporterGreen Fluorescent ProteinsHomologous RecombinationHumansImmunoglobulin Switch RegionLuminescent ProteinsMiceModels, GeneticMolecular Sequence DataNucleic Acid HeteroduplexesRecombinational DNA RepairRepetitive Sequences, Nucleic AcidSequence DeletionSequence Homology, Nucleic AcidSomatic Hypermutation, ImmunoglobulinConceptsHomologous recombinationCytidine deaminase proteinSequence repeatsCytidine deaminationDNA end resectionHundreds of basesAnalysis of recombinantsVertebrate cellsGene conversionRepeat recombinationEnd resectionHolliday junctionsHomologous sequencesSequence homologyReporter transgeneStrand invasionIntergenic deletionRecombinogenic activityImmunoglobulin lociRepeatsSomatic hypermutationHeteroduplex formationRecombinationProteinDeamination
2002
Site-directed recombination via bifunctional PNA–DNA conjugates
Rogers FA, Vasquez KM, Egholm M, Glazer PM. Site-directed recombination via bifunctional PNA–DNA conjugates. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 16695-16700. PMID: 12461167, PMCID: PMC139206, DOI: 10.1073/pnas.262556899.Peer-Reviewed Original ResearchConceptsSite-directed recombinationDNA repairNucleotide excision repair factorsExcision repair factorsSite-specific DNASite-specific recombinationHuman cell-free extractsDonor DNA fragmentsRepair factorsMammalian cellsGenetic manipulationCell-free extractsTarget genesSite-directed mannerReporter geneStrand invasionPlasmid substratesDNA fragmentsDefective geneDonor DNAWatson-Crick base pairingDuplex DNAGenesTarget plasmidDNA
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