2023
Site-specific crosslinking reveals Phosphofructokinase-L inhibition drives self-assembly and attenuation of protein interactions
Sivadas A, McDonald E, Shuster S, Davis C, Plate L. Site-specific crosslinking reveals Phosphofructokinase-L inhibition drives self-assembly and attenuation of protein interactions. Advances In Biological Regulation 2023, 90: 100987. PMID: 37806136, PMCID: PMC11108229, DOI: 10.1016/j.jbior.2023.100987.Peer-Reviewed Original ResearchConceptsProtein interactionsProtein-protein interactionsSite-specific crosslinkingSite-specific incorporationMetabolic proteinsRegulated stepProtein assembliesCentral enzymePuncta formationMetabolic fluxHuman cellsInterface 2Amino acidsCytoskeletonLiver isoformCentral roleCompartmentalizationGlycolysisActive siteInteractorsInhibitionInterface 1IsoformsProteinCitrate inhibitionRecoding UAG to selenocysteine in Saccharomyces cerevisiae
Hoffman K, Chung C, Mukai T, Krahn N, Jiang H, Balasuriya N, O'Donoghue P, Söll D. Recoding UAG to selenocysteine in Saccharomyces cerevisiae. RNA 2023, 29: 1400-1410. PMID: 37279998, PMCID: PMC10573291, DOI: 10.1261/rna.079658.123.Peer-Reviewed Original ResearchConceptsSelenoprotein productionYeast expression systemSeryl-tRNA synthetaseSite-specific incorporationEukaryotic relativesKingdom FungiSelenocysteine synthaseSelenophosphate synthetaseBiosynthesis pathwayEukaryotic selenoproteinsMetabolic engineeringBiosynthetic pathwayPathway componentsExpression systemReductase enzymeTRNASaccharomycesYeastTranslation componentsSpecific sitesFacile productionUnique chemicalSynthetasePathwayFirst demonstration
2022
The tRNA discriminator base defines the mutual orthogonality of two distinct pyrrolysyl-tRNA synthetase/tRNAPyl pairs in the same organism
Zhang H, Gong X, Zhao Q, Mukai T, Vargas-Rodriguez O, Zhang H, Zhang Y, Wassel P, Amikura K, Maupin-Furlow J, Ren Y, Xu X, Wolf YI, Makarova KS, Koonin EV, Shen Y, Söll D, Fu X. The tRNA discriminator base defines the mutual orthogonality of two distinct pyrrolysyl-tRNA synthetase/tRNAPyl pairs in the same organism. Nucleic Acids Research 2022, 50: gkac271-. PMID: 35466371, PMCID: PMC9071458, DOI: 10.1093/nar/gkac271.Peer-Reviewed Original ResearchConceptsGenetic code expansionCode expansionDistinct non-canonical amino acidsOrthogonal aminoacyl-tRNA synthetase/tRNA pairsAminoacyl-tRNA synthetase/tRNA pairsPyrrolysyl-tRNA synthetase/Halophilic archaeon Haloferax volcaniiAdditional coding capacityDistinct noncanonical amino acidsNon-canonical amino acidsArchaeon Haloferax volcaniiDiscriminator baseAmino acidsPyrrolysyl-tRNA synthetaseNoncanonical amino acidsSite-specific incorporationMotif 2 loopSingle base changeDistinct tRNAsTRNA pairsHaloferax volcaniiUAA codonGenetic codeDiscriminator basesTRNA structure
2021
Chemoselective restoration of para-azido-phenylalanine at multiple sites in proteins
Arranz-Gibert P, Vanderschuren K, Haimovich A, Halder A, Gupta K, Rinehart J, Isaacs FJ. Chemoselective restoration of para-azido-phenylalanine at multiple sites in proteins. Cell Chemical Biology 2021, 29: 1046-1052.e4. PMID: 34965380, PMCID: PMC10173106, DOI: 10.1016/j.chembiol.2021.12.002.Peer-Reviewed Original ResearchConceptsProtein functionalizationChemical moietiesDiverse chemical moietiesDiverse chemical groupsSite-specific incorporationClick reactionOff-target modificationsDiazotransfer reactionAzide moietyAzide reductionNonstandard amino acidsChemical groupsFunctionalizationMaterials scienceMoietyPAZFFunction of proteinsReactionAzideBroad applicationsPhysiological conditionsParaChemistryAmino acidsKey limitations
1992
Site-Specific Incorporation of Novel Backbone Structures into Proteins
Ellman J, Mendel D, Schultz P. Site-Specific Incorporation of Novel Backbone Structures into Proteins. Science 1992, 255: 197-200. PMID: 1553546, DOI: 10.1126/science.1553546.Peer-Reviewed Original ResearchConceptsAmino acidsProtein biosynthetic machineryT4 lysozymeBroad substrate specificitySite-specific incorporationUnnatural amino acidsBiosynthetic machineryProtein stabilitySubstrate specificityAmino acid analoguesN-alkyl amino acidsNovel backbone structuresAlpha-disubstituted amino acidsBackbone structureStructural perturbationsSide-chain substitutionAcid analoguesAcidMachineryProteinLysozymeAlanineLactic acidWide range
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply