2024
Effect of Incomplete Cryoablation and Matrix Metalloproteinase Inhibition on Intratumoral CD8+ T-Cell Infiltration in Murine Hepatocellular Carcinoma.
Shewarega A, Santana J, Nam D, Berz A, Tefera J, Kahl V, Mishra S, Coman D, Duncan J, Roberts S, Wetter A, Madoff D, Chapiro J. Effect of Incomplete Cryoablation and Matrix Metalloproteinase Inhibition on Intratumoral CD8+ T-Cell Infiltration in Murine Hepatocellular Carcinoma. Radiology 2024, 310: e232365. PMID: 38349244, PMCID: PMC10902598, DOI: 10.1148/radiol.232365.Peer-Reviewed Original ResearchConceptsT cell infiltrationCD8<sup>+</sup> T cellsMatrix metalloproteinase inhibitionT cellsHepatocellular carcinomaMatrix metalloproteinase inhibitorsMatrix metalloproteinasesResidual tumorCXCR3<sup>+</sup> CD8<sup>+</sup> T cellsCytotoxic CD8<sup>+</sup> T cell infiltrationIntratumoral CD8+ T cell infiltrationCD8+ T cell infiltrationCD8<sup>+</sup> T cell infiltrationMouse model of hepatocellular carcinomaEarly-stage hepatocellular carcinomaImage-guided tumor ablationUnpaired Student's <i>t</i> testModel of hepatocellular carcinomaFirst-line therapyMurine hepatocellular carcinomaT cell subsetsTumor-associated macrophagesMurine HCC modelLocal immune responseFemale BALB/c mice
2022
CineCT platform for in vivo and ex vivo measurement of 3D high resolution Lagrangian strains in the left ventricle following myocardial infarction and intramyocardial delivery of theranostic hydrogel
Midgett D, Thorn S, Ahn S, Uman S, Avendano R, Melvinsdottir I, Lysyy T, Kim J, Duncan J, Humphrey J, Papademetris X, Burdick J, Sinusas A. CineCT platform for in vivo and ex vivo measurement of 3D high resolution Lagrangian strains in the left ventricle following myocardial infarction and intramyocardial delivery of theranostic hydrogel. Journal Of Molecular And Cellular Cardiology 2022, 166: 74-90. PMID: 35227737, PMCID: PMC9035115, DOI: 10.1016/j.yjmcc.2022.02.004.Peer-Reviewed Original Research
2020
Idarubicin-Loaded ONCOZENE Drug-Eluting Bead Chemoembolization in a Rabbit Liver Tumor Model: Investigating Safety, Therapeutic Efficacy, and Effects on Tumor Microenvironment
Borde T, Gaupp F, Geschwind JF, Savic LJ, Miszczuk M, Rexha I, Adam L, Walsh JJ, Huber S, Duncan JS, Peters DC, Sinusas A, Schlachter T, Gebauer B, Hyder F, Coman D, van Breugel JMM, Chapiro J. Idarubicin-Loaded ONCOZENE Drug-Eluting Bead Chemoembolization in a Rabbit Liver Tumor Model: Investigating Safety, Therapeutic Efficacy, and Effects on Tumor Microenvironment. Journal Of Vascular And Interventional Radiology 2020, 31: 1706-1716.e1. PMID: 32684417, PMCID: PMC7541537, DOI: 10.1016/j.jvir.2020.04.010.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibiotics, AntineoplasticBiosensing TechniquesCell Line, TumorCell ProliferationChemoembolization, TherapeuticDiffusion Magnetic Resonance ImagingHydrogen-Ion ConcentrationIdarubicinLiver Neoplasms, ExperimentalMaleMicrospheresMultidetector Computed TomographyParticle SizeRabbitsTumor MicroenvironmentConceptsMultiparametric magnetic resonanceRabbit liver tumor modelDiffusion-weighted imagingLiver tumor modelDEE chemoembolizationDrug-eluting embolic transarterial chemoembolizationTumor microenvironmentTumor modelMale New Zealand white rabbitsTumor acidosisNew Zealand white rabbitsVX2 liver tumorsZealand white rabbitsLaboratory parametersTransarterial chemoembolizationBead chemoembolizationMultiparametric MRDCE MR imagingLiver enzymesPostprocedural increaseIntratumoral hypoxiaLiver tumorsEntire lesionTherapeutic mechanismChemoembolizationMolecular MRI of the Immuno-Metabolic Interplay in a Rabbit Liver Tumor Model: A Biomarker for Resistance Mechanisms in Tumor-targeted Therapy?
Savic LJ, Doemel LA, Schobert IT, Montgomery RR, Joshi N, Walsh JJ, Santana J, Pekurovsky V, Zhang X, Lin M, Adam L, Boustani A, Duncan J, Leng L, Bucala RJ, Goldberg SN, Hyder F, Coman D, Chapiro J. Molecular MRI of the Immuno-Metabolic Interplay in a Rabbit Liver Tumor Model: A Biomarker for Resistance Mechanisms in Tumor-targeted Therapy? Radiology 2020, 296: 575-583. PMID: 32633675, PMCID: PMC7434651, DOI: 10.1148/radiol.2020200373.Peer-Reviewed Original ResearchConceptsImmuno-oncologic therapiesConventional transarterial chemoembolizationTransarterial chemoembolizationIntratumoral immune cell infiltrationMR spectroscopyRabbit liver tumor modelPrussian blue iron stainingAntigen-presenting immune cellsIntra-arterial infusionImmune cell infiltrationNew Zealand white rabbitsLiver tumor modelImmune cell exclusionLiver cancer modelContrast material administrationT2-weighted MRIZealand white rabbitsT2-weighted imagingResistance mechanismsImmunosuppressive tumorHLA-DRCell infiltrationImmune cellsImmunohistochemistry stainingRing enhancementImaging of Injectable Hydrogels Delivered into Myocardium with SPECT/CT
Uman S, Wang LL, Thorn SL, Liu Z, Duncan JS, Sinusas AJ, Burdick JA. Imaging of Injectable Hydrogels Delivered into Myocardium with SPECT/CT. Advanced Healthcare Materials 2020, 9: e2000294. PMID: 32543053, PMCID: PMC7482444, DOI: 10.1002/adhm.202000294.Peer-Reviewed Original ResearchConceptsInjectable hydrogelsMyocardial infarctionSelf-healing hydrogelsHydrogel mechanical propertiesHybrid single-photon emissionHybrid SPECT/CT imagingMechanical propertiesTomography perfusion imagingAcute porcine modelSPECT/CT imagingSPECT/CTSingle photon emissionInjection forceHyaluronic acid hydrogelsPattern of deliveryTomography/HydrogelsPerfusion defectsPerfusion imagingExcellent radiopacityPorcine modelAcid hydrogelsCT imagingHydrogel deliveryHydrogel injectionSparse Data–Driven Learning for Effective and Efficient Biomedical Image Segmentation
Onofrey JA, Staib LH, Huang X, Zhang F, Papademetris X, Metaxas D, Rueckert D, Duncan JS. Sparse Data–Driven Learning for Effective and Efficient Biomedical Image Segmentation. Annual Review Of Biomedical Engineering 2020, 22: 1-27. PMID: 32169002, PMCID: PMC9351438, DOI: 10.1146/annurev-bioeng-060418-052147.Peer-Reviewed Original ResearchRegional myocardial strain analysis via 2D speckle tracking echocardiography: validation with sonomicrometry and correlation with regional blood flow in the presence of graded coronary stenoses and dobutamine stress
Stendahl JC, Parajuli N, Lu A, Boutagy NE, Guerrera N, Alkhalil I, Lin BA, Staib LH, O’Donnell M, Duncan JS, Sinusas AJ. Regional myocardial strain analysis via 2D speckle tracking echocardiography: validation with sonomicrometry and correlation with regional blood flow in the presence of graded coronary stenoses and dobutamine stress. Cardiovascular Ultrasound 2020, 18: 2. PMID: 31941514, PMCID: PMC6964036, DOI: 10.1186/s12947-019-0183-x.Peer-Reviewed Original ResearchConceptsSpeckle tracking echocardiographyLow-dose dobutamine stressPost-systolic indexDobutamine stressCoronary stenosisBlood flow measurementsTracking echocardiographyAnesthetized open-chest dogsCircumferential strainLow-dose dobutamineMid-left anteriorModerate coronary stenosisOpen-chest dogsRegional blood flowAcute canine modelMyocardial strain analysisMicrosphere blood flow measurementsLAD stenosisMolecular Imaging of Extracellular Tumor pH to Reveal Effects of Locoregional Therapy on Liver Cancer Microenvironment
Savic LJ, Schobert I, Peters D, Walsh JJ, Laage-Gaupp F, Hamm CA, Tritz N, Doemel LA, Lin M, Sinusas A, Schlachter T, Duncan JS, Hyder F, Coman D, Chapiro J. Molecular Imaging of Extracellular Tumor pH to Reveal Effects of Locoregional Therapy on Liver Cancer Microenvironment. Clinical Cancer Research 2020, 26: 428-438. PMID: 31582517, PMCID: PMC7244230, DOI: 10.1158/1078-0432.ccr-19-1702.Peer-Reviewed Original ResearchConceptsMR spectroscopic imagingLocoregional therapyLiver cancer microenvironmentConventional transarterial chemoembolizationNew Zealand white rabbitsTumor pHMost liver tumorsZealand white rabbitsMolecular imaging paradigmsPositive therapeutic outcomesTumor residualsTransarterial chemoembolizationTumor devascularizationHistopathologic markersViable tumorSurrogate biomarkerLiver tumorsLiver cancerTumor enhancementLiver parenchymaMetabolic markersMultiparametric MRITherapeutic outcomesHIF-1αVX2 tumors
2019
Extracellular pH mapping of liver cancer on a clinical 3T MRI scanner
Coman D, Peters DC, Walsh JJ, Savic LJ, Huber S, Sinusas AJ, Lin M, Chapiro J, Constable RT, Rothman DL, Duncan JS, Hyder F. Extracellular pH mapping of liver cancer on a clinical 3T MRI scanner. Magnetic Resonance In Medicine 2019, 83: 1553-1564. PMID: 31691371, PMCID: PMC7244229, DOI: 10.1002/mrm.28035.Peer-Reviewed Original ResearchFlow network tracking for spatiotemporal and periodic point matching: Applied to cardiac motion analysis
Parajuli N, Lu A, Ta K, Stendahl J, Boutagy N, Alkhalil I, Eberle M, Jeng GS, Zontak M, O'Donnell M, Sinusas AJ, Duncan JS. Flow network tracking for spatiotemporal and periodic point matching: Applied to cardiac motion analysis. Medical Image Analysis 2019, 55: 116-135. PMID: 31055125, PMCID: PMC6939679, DOI: 10.1016/j.media.2019.04.007.Peer-Reviewed Original ResearchConceptsDeformation/strainExcellent tracking accuracyEntire cardiac cycleTracking accuracyCardiac motion analysisAccurate estimationSurface pointsEchocardiographic image sequencesLV motionDisplacementMotion analysisImage sequencesCardiac cyclePoint matchingMotionConsecutive framesEstimationNetwork trackingImportant characteristicsSignificant promiseSchemeGood correlationFlow
2014
Radial Basis Functions for Combining Shape and Speckle Tracking in 4D Echocardiography
Compas C, Wong EY, Huang X, Sampath S, Lin BA, Pal P, Papademetris X, Thiele K, Dione DP, Stacy M, Staib LH, Sinusas AJ, O'Donnell M, Duncan JS. Radial Basis Functions for Combining Shape and Speckle Tracking in 4D Echocardiography. IEEE Transactions On Medical Imaging 2014, 33: 1275-1289. PMID: 24893257, PMCID: PMC4283552, DOI: 10.1109/tmi.2014.2308894.Peer-Reviewed Original Research
2013
Contour tracking in echocardiographic sequences via sparse representation and dictionary learning
Huang X, Dione DP, Compas CB, Papademetris X, Lin BA, Bregasi A, Sinusas AJ, Staib LH, Duncan JS. Contour tracking in echocardiographic sequences via sparse representation and dictionary learning. Medical Image Analysis 2013, 18: 253-271. PMID: 24292554, PMCID: PMC3946038, DOI: 10.1016/j.media.2013.10.012.Peer-Reviewed Original ResearchConceptsContour trackerSparse representationEchocardiographic sequencesRegion-based level set segmentationLevel set segmentationLocal image appearanceManual tracingExpert manual tracingsMultiscale sparse representationImage sequencesSegmentation resultsAppearance modelSpatiotemporal priorsFirst frameMultilevel informationHuman data setsEjection fraction estimatesLocal appearanceImage appearanceDictionary learningShape modelContour trackingManual resultsData setsContour estimationA Multiple Hypothesis Based Method for Particle Tracking and Its Extension for Cell Segmentation
Liang L, Shen H, Rompolas P, Greco V, De Camilli P, Duncan JS. A Multiple Hypothesis Based Method for Particle Tracking and Its Extension for Cell Segmentation. Lecture Notes In Computer Science 2013, 23: 98-109. PMID: 24683961, PMCID: PMC4122512, DOI: 10.1007/978-3-642-38868-2_9.Peer-Reviewed Original ResearchSegmentation of 4D Echocardiography Using Stochastic Online Dictionary Learning
Huang X, Dione DP, Lin BA, Bregasi A, Sinusas AJ, Duncan JS. Segmentation of 4D Echocardiography Using Stochastic Online Dictionary Learning. Lecture Notes In Computer Science 2013, 16: 57-65. PMID: 24505744, DOI: 10.1007/978-3-642-40760-4_8.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsAnimalsArtificial IntelligenceData Interpretation, StatisticalDogsEchocardiography, Four-DimensionalImage EnhancementImage Interpretation, Computer-AssistedMyocardial InfarctionPattern Recognition, AutomatedReproducibility of ResultsSensitivity and SpecificityStochastic ProcessesSubtraction Technique
2012
Assessment of left ventricular 2D flow pathlines during early diastole using spatial modulation of magnetization with polarity alternating velocity encoding: A study in normal volunteers and canine animals with myocardial infarction
Zhang Z, Friedman D, Dione DP, Lin BA, Duncan JS, Sinusas AJ, Sampath S. Assessment of left ventricular 2D flow pathlines during early diastole using spatial modulation of magnetization with polarity alternating velocity encoding: A study in normal volunteers and canine animals with myocardial infarction. Magnetic Resonance In Medicine 2012, 70: 766-775. PMID: 23044637, PMCID: PMC3844046, DOI: 10.1002/mrm.24517.Peer-Reviewed Original Research
2011
Segmentation of 3D radio frequency echocardiography using a spatio-temporal predictor
Pearlman PC, Tagare HD, Lin BA, Sinusas AJ, Duncan JS. Segmentation of 3D radio frequency echocardiography using a spatio-temporal predictor. Medical Image Analysis 2011, 16: 351-360. PMID: 22078842, PMCID: PMC3267850, DOI: 10.1016/j.media.2011.09.002.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsAnimalsComputer SimulationDogsEchocardiography, Three-DimensionalHeart VentriclesImage EnhancementImage Interpretation, Computer-AssistedImaging, Three-DimensionalModels, CardiovascularModels, StatisticalPattern Recognition, AutomatedReproducibility of ResultsSensitivity and SpecificitySubtraction TechniqueConceptsLeft ventricular endocardial boundarySpatio-temporal predictorsStandard level setRF dataSpatio-temporal coherenceNeighboring framesImage sequencesBoundary detectionMultiple framesImage inhomogeneitySegmentationEndocardial boundaryGeometric constraintsManual tracingRF ultrasoundAlgorithmLevel setsEchocardiographic imagesFrameConditional modelLinear predictorTrackingSpatial modelImagesRobustnessTargeted Imaging of the Spatial and Temporal Variation of Matrix Metalloproteinase Activity in a Porcine Model of Postinfarct Remodeling
Sahul ZH, Mukherjee R, Song J, McAteer J, Stroud RE, Dione DP, Staib L, Papademetris X, Dobrucki LW, Duncan JS, Spinale FG, Sinusas AJ. Targeted Imaging of the Spatial and Temporal Variation of Matrix Metalloproteinase Activity in a Porcine Model of Postinfarct Remodeling. Circulation Cardiovascular Imaging 2011, 4: 381-391. PMID: 21505092, PMCID: PMC3140564, DOI: 10.1161/circimaging.110.961854.Peer-Reviewed Original Research3-D Reconstruction of Microtubules from Multi-Angle Total Internal Reflection Fluorescence Microscopy Using Bayesian Framework
Yang Q, Karpikov A, Toomre D, Duncan JS. 3-D Reconstruction of Microtubules from Multi-Angle Total Internal Reflection Fluorescence Microscopy Using Bayesian Framework. IEEE Transactions On Image Processing 2011, 20: 2248-2259. PMID: 21324778, DOI: 10.1109/tip.2011.2114359.Peer-Reviewed Original ResearchConceptsEvanescent fieldDifferent penetration depthsTIRF imagesPenetration depthSamples of microtubulesLaser beamTotal internal reflection fluorescence microscopyAxial resolutionReflection fluorescence microscopyLarge radiusIncident angleMulti-angle total internal reflection fluorescence microscopyElectron microscopy imagesTIRF dataSmall radiusTracking of microtubulesMicroscopy imagesFluorescence microscopyMicroscopyRadiusReconstruction resultsCurvilinear characteristicsZ-dimensionExperimental calibrationMicrotubule curvatureSegmentation of 3D RF Echocardiography Using a Multiframe Spatio-temporal Predictor
Pearlman PC, Tagare HD, Lin BA, Sinusas AJ, Duncan JS. Segmentation of 3D RF Echocardiography Using a Multiframe Spatio-temporal Predictor. Lecture Notes In Computer Science 2011, 22: 37-48. PMID: 21761644, DOI: 10.1007/978-3-642-22092-0_4.Peer-Reviewed Original ResearchConceptsLeft ventricular endocardial boundarySpatio-temporal predictorsStandard level setSpatio-temporal coherenceNeighboring framesImage sequencesBoundary detectionRF dataMultiple framesImage inhomogeneitySegmentationEndocardial boundaryGeometric constraintsManual tracingRF ultrasoundLevel setsConditional modelEchocardiographic imagesFrameLinear predictorAlgorithmTrackingSpatial modelImagesRobustness
2010
A coupled deformable model for tracking myocardial borders from real-time echocardiography using an incompressibility constraint
Zhu Y, Papademetris X, Sinusas AJ, Duncan JS. A coupled deformable model for tracking myocardial borders from real-time echocardiography using an incompressibility constraint. Medical Image Analysis 2010, 14: 429-448. PMID: 20350833, PMCID: PMC4318707, DOI: 10.1016/j.media.2010.02.005.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsAnimalsArtificial IntelligenceComputer SystemsDogsEchocardiography, Three-DimensionalElasticity Imaging TechniquesHumansImage EnhancementImage Interpretation, Computer-AssistedPattern Recognition, AutomatedReproducibility of ResultsSensitivity and SpecificitySubtraction TechniqueConceptsDeformable modelImage-derived informationLV endocardial boundariesImage acquisition techniquesFinal segmentationAutomatic algorithmGround truthManual segmentationVolumetric imagesSegmentationSynthetic dataEndocardial boundaryNumber of effortsMyocardial bordersEpicardial boundariesAcquisition techniquesInstantaneous acquisitionConstraintsImagesEchocardiographic imagesSetSpeckle statisticsAlgorithmReal-time echocardiography