2012
Utility of peripheral blood flow cytometry in differentiating low grade versus high grade myelodysplastic syndromes (MDS) and in the evaluation of cytopenias.
Rashidi HH, Xu X, Wang HY, Shafi NQ, Rameshkumar K, Messer K, Smith BR, Rose MG. Utility of peripheral blood flow cytometry in differentiating low grade versus high grade myelodysplastic syndromes (MDS) and in the evaluation of cytopenias. International Journal Of Clinical And Experimental Pathology 2012, 5: 224-30. PMID: 22558477, PMCID: PMC3341683.Peer-Reviewed Original ResearchConceptsHigh-grade myelodysplastic syndromeChronic myelomonocytic leukemiaPeripheral blood flow cytometryEvaluation of cytopeniasBlood flow cytometryMyelodysplastic syndromeFlow cytometryDifferential diagnosisHigh-risk myelodysplastic syndromeLow-grade myelodysplastic syndromesRisk myelodysplastic syndromesCMML patientsMyelomonocytic leukemiaDiagnostic utilityCytopeniasPatientsCytometrySyndromeLow gradeDiagnosisLeukemia
2007
Flow cytometric analysis of myelomonocytic cells by a pattern recognition approach is sensitive and specific in diagnosing myelodysplastic syndrome and related marrow diseases: Emphasis on a global evaluation and recognition of diagnostic pitfalls
Stachurski D, Smith BR, Pozdnyakova O, Andersen M, Xiao Z, Raza A, Woda BA, Wang A. Flow cytometric analysis of myelomonocytic cells by a pattern recognition approach is sensitive and specific in diagnosing myelodysplastic syndrome and related marrow diseases: Emphasis on a global evaluation and recognition of diagnostic pitfalls. Leukemia Research 2007, 32: 215-224. PMID: 17675229, DOI: 10.1016/j.leukres.2007.06.012.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDBone Marrow CellsBone Marrow DiseasesFemaleFlow CytometryHumansMaleMonocytesMyelodysplastic SyndromesRetrospective StudiesSensitivity and SpecificityConceptsMyelodysplastic syndromeFlow cytometryDiagnostic pitfallsMyelomonocytic cellsMDS/MPDClonal cytogenetic abnormalitiesGlobal evaluationImmunophenotypic aberranciesRetrospective reviewMorphological dysplasiaMarrow diseaseMyeloproliferative diseaseDiagnostic utilityCytogenetic abnormalitiesDisease casesMyeloid diseasesCytometric analysisOverall sensitivityAbnormalitiesDiseaseOverall abnormalitySyndromeIntermediate category
2004
Real-Time Quantitative Reverse Transcription-PCR for Cyclin D1 mRNA in Blood, Marrow, and Tissue Specimens for Diagnosis of Mantle Cell Lymphoma
Howe JG, Crouch J, Cooper D, Smith BR. Real-Time Quantitative Reverse Transcription-PCR for Cyclin D1 mRNA in Blood, Marrow, and Tissue Specimens for Diagnosis of Mantle Cell Lymphoma. Clinical Chemistry 2004, 50: 80-87. PMID: 14633913, DOI: 10.1373/clinchem.2003.024695.Peer-Reviewed Original ResearchConceptsMantle cell lymphomaReal-time reverse transcription PCRCases of MCLCyclin D1 mRNAQuantitative RT-PCRLymphoproliferative diseaseReverse transcription-PCRCell lymphomaD1 mRNAMicroglobulin mRNADiagnosis of MCLTranscription-PCRCyclin D1 mRNA expressionB cell-specific markersCritical diagnostic markerReal-time quantitative RT-PCRD1 mRNA expressionTime quantitative reverse transcription PCRQuantitative reverse transcription PCROvert diseaseMarrow specimensAmbiguous immunophenotypeCD19 mRNAMalignant cellsTissue specimens
2003
Real-time Quantitative RT-PCR of Cyclin D1 mRNA in Mantle Cell Lymphoma: Comparison with FISH and Immunohistochemistry
Hui* P, Howe* J, Crouch J, Nimmakayalu M, Qumsiyeh MB, Tallini G, Flynn SD, Smith BR. Real-time Quantitative RT-PCR of Cyclin D1 mRNA in Mantle Cell Lymphoma: Comparison with FISH and Immunohistochemistry. Leukemia & Lymphoma 2003, 44: 1385-1394. PMID: 12952233, DOI: 10.1080/1042819031000079168.Peer-Reviewed Original ResearchCyclin D1Diagnosis, DifferentialFalse Negative ReactionsHumansImmunohistochemistryIn Situ Hybridization, FluorescenceLymphoma, B-CellLymphoma, Mantle-CellReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerRNA, NeoplasmSensitivity and SpecificityTranslocation, Genetic
1998
Morphologic, Immunophenotypic, and Molecular Evaluation of Bone Marrow Involvement in Non-Hodgkin's Lymphoma
Crotty P, Smith B, Tallini G. Morphologic, Immunophenotypic, and Molecular Evaluation of Bone Marrow Involvement in Non-Hodgkin's Lymphoma. Applied Immunohistochemistry & Molecular Morphology 1998, 7: 90-95. PMID: 9785007, DOI: 10.1097/00019606-199804000-00005.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDBiomarkers, TumorBone MarrowBone Marrow ExaminationClone CellsFlow CytometryGene Rearrangement, B-LymphocyteGene Rearrangement, T-LymphocyteHumansImmunophenotypingLymphoma, B-CellLymphoma, Non-HodgkinLymphoma, T-CellNeoplasm ProteinsNeoplastic Stem CellsPolymerase Chain ReactionSensitivity and SpecificityConceptsPrimer polymerase chain reactionMarrow involvementT-cell neoplasiaFlow cytometryB-cell neoplasiaObjective evidenceT-cell receptor gene rearrangementsBone marrow involvementConsensus primer polymerase chain reactionNon-Hodgkin lymphomaChronic lymphocytic leukemiaB-cell lymphomaT-cell neoplasmsB-cell neoplasmsReceptor gene rearrangementsDetection of clonalityDetectable diseaseHodgkin's lymphomaPolymerase chain reactionLymphocytic leukemiaApparent diseaseLymphomaPatientsImportant adjunctNeoplasia