Fluorescence Activated Cell Sorting is available on seven cytometers and performed mostly by Core Facility personnel. To make an appointment user must reserve time via e-mail and have a brief discussion about the experiment with cell sorter operator. It is important to provide information about the source and Biosafety level of the cells, fluorochromes used for staining and estimated amount of material.
To sort primary human materials on any of our Aria sorters, documented proof (such as a test slip) of patient's negative results of testing for HIV, Hepatitis B and Hepatitis C has to be provided prior to sort.
For sorting unscreened human material please contact Geoffrey.Lyon@yale.edu.
No internet scheduling is available for cell sorting, scheduling website information is for viewing purpose only. Cancellations also cannot be made through the internet although technically possible. Users must notify staff about canceling by e-mailing or calling. Please refer to Policies to learn about our scheduling and cancellation rules.
Depending on the cell type being investigated, there are a variety of procedures for preparing cells. Before labeling cells for sorting the antibody's concentration may need to be optimized, and appropriate negative and positive controls are needed. It is important to bring cells filtered and in single cell suspension, at proper concentration and stained with fluorochromes that are suitable for the cytometer of choice.
No cell preparation or staining is performed at our facility.
Detailed information about each sorter's capabilities is available through the links on the left column. All facility cytometers can be viewed on the Fluorochromes and filters chart to help choose the appropriate machine.