Sony-iCyt Reflection
Room: TAC S617
Contact:Cameron.Godecke@yale.edu
- High speed Cell Sorter
- Four lasers: 405 nm, 488 nm, 532 nm and 640 nm excitation lines
- Can be RFP or PE from the green laser. PerCP will not work because of the too high laser power
- Two sort heads set with 70, 100 or 126 micron nozzles
- Can sort into 96 and 384-well plates
- Biosafety level 2 (Baker SterilGuardIII biological safety cabinet)
- Acquisition: Verity SoftwareWinlist
Violet Laser-405 nm
PMT | Filter | Fluorochrome | |
455/50
| Pacific Blue, Alexa Fluor 405
| ||
585/40
| Pacific Orange | ||
| 625/30
| Q-dot
|
Blue Laser - 488nm
PMT | Filter | Fluorochrome |
535/50 | GFP,YFP, FITC, Alexa Fluor 488 | |
710/40 | PerCP-Cy5.5, PE-Cy5.5 | |
780/60 | PE-Cy7 |
Green laser - 532 nm
PMT | Filter | Fluorochrome |
605/70 | RFP
| |
585/40 | PE, DsRed, Alexa Fluor 532
| |
700/75 | PE-Cy5, PerCP-Cy5.5 | |
780/60 | PE-Cy7 |
Red laser - 640 nm
PMT | Filter | Fluorochrome | ||||
665/30 | APC, Alexa Fluor 647, 635
| |||||
| 710/40
| Alexa Fluor 700, 660
| |||||
780/60 | APC-Cy7 |


