Sony-iCyt Reflection

Room: TAC S617
Contact:Cameron.Godecke@yale.edu

Manufacturer's website

  • High speed Cell Sorter
  • Four lasers: 405 nm, 488 nm, 532 nm and 640 nm excitation lines
  • Can be RFP or PE from the green laser. PerCP will not work because of the too high laser power
  • Two sort heads set with 70, 100 or 126 micron nozzles
  • Can sort into 96 and 384-well plates
  • Biosafety level 2 (Baker SterilGuardIII biological safety cabinet)
  • Acquisition: Verity SoftwareWinlist

Violet Laser-405 nm

PMT

Filter

Fluorochrome

455/50   

Pacific Blue, Alexa Fluor 405

585/40

Pacific Orange


625/30
Q-dot

Blue Laser - 488nm

PMT

Filter

Fluorochrome

535/50

GFP,YFP, FITC, Alexa Fluor 488

710/40

PerCP-Cy5.5, PE-Cy5.5

780/60

PE-Cy7

Green laser - 532 nm

PMT

Filter

Fluorochrome

605/70

RFP

585/40

PE, DsRed, Alexa Fluor 532

700/75

PE-Cy5, PerCP-Cy5.5

780/60

PE-Cy7

Red laser - 640 nm

PMT

Filter





Fluorochrome


665/30





APC, Alexa Fluor 647, 635


710/40




Alexa Fluor 700, 660

780/60





APC-Cy7