BD FACSAria

Room: TAC S617

Contact:Ewa.Menet@yale.edu (203)785-2851 (TAC S617B) or (203)785-7958 (office)

Technical Specifications

  • High speed cell sorter
  • Multilaser multiparameter analysis cytometer
  • Three lasers, 10 colors
  • Digital data collection using FACS DIVA software
  • Automatic compensation (compensation controls are still needed)
  • Capable of sorting human samples (equipped with Aerosol Evacuation System)
  • Single cell sorting (all sizes of plates or microscope slides)
  • Sterile sorting
  • 4-way sorting

Violet Laser - 407nm

PMT

Bandpass

Long Pass

Fluorochrome

A

530/30

502LP

Alexa Fluor 430, CFP

B

450/40

Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, Marina Blue

Blue Laser - 488nm

PMT

Bandpass

Long Pass

Fluorochrome

A

780/60

735LP

PE-Cy7, PE-Alexa Fluor 750

B

695/40

655LP

PI (with PE), PerCP, PerCP-Cy5.5, PE-Cy5, EMA

C

610/20

595LP

PE-Texas Red, PI (with PE and PE-Cy5.5)

D

576/26

556LP

PE, PI (with PerCP)

E

530/30

502LP

FITC, GFP, CFSE, YFP, Alexa Fluor 488, SYBR-14

F

488/10

SSC

Red Laser - 633nm

PMT

Bandpass

Long Pass

Fluorochrome

A

780/60

735LP

APC-Cy7, APC-Alexa 750

B

710/25

685LP

Alexa Fluor 680, Alexa Fluor 700

C

660/20

APC, Alexa Fluor 647

*Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to determine which fluorochromes cannot be used together due to high spectral overlaps.