BD FACSAria
Contact: Thomas.Taylor@yale.edu or (203)737-4715
- High speed cell sorter
- Multilaser multiparameter analysis cytometer
- Three lasers, 10 colors
- Digital data collection using FACS DIVA software
- Automatic compensation (compensation controls are still needed)
- Capable of sorting human samples (equipped with Aerosol Evacuation System)
- Single cell sorting (all sizes of plates or microscope slides)
- Sterile sorting
- 4-way sorting
Violet Laser - 407nm
PMT | Bandpass | Long Pass | Fluorochrome |
A | 530/30 | 502LP | Alexa Fluor 430, CFP |
B | 450/40 | Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, Marina Blue |
Blue Laser - 488nm
PMT | Bandpass | Long Pass | Fluorochrome |
A | 780/60 | 735LP | PE-Cy7, PE-Alexa Fluor 750 |
B | 695/40 | 655LP | PI (with PE), PerCP, PerCP-Cy5.5, PE-Cy5, EMA |
C | 610/20 | 595LP | PE-Texas Red, PI (with PE and PE-Cy5.5) |
D | 576/26 | 556LP | PE, PI (with PerCP) |
E | 530/30 | 502LP | FITC, GFP, CFSE, YFP, Alexa Fluor 488, SYBR-14 |
F | 488/10 | SSC |
Red Laser - 633nm
PMT | Bandpass | Long Pass | Fluorochrome |
A | 780/60 | 735LP | APC-Cy7, APC-Alexa 750 |
B | 710/25 | 685LP | Alexa Fluor 680, Alexa Fluor 700 |
C | 660/20 | APC, Alexa Fluor 647 |
*Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to determine which fluorochromes cannot be used together due to high spectral overlaps.
