Fluorescence-Activated Cell Sorting Facility

Department of Laboratory Medicine. Co-sponsored by Departments of Immunobiology and Internal Medicine, the Yale Cancer Center, and HHMI.

Our mission is to provide comprehensive flow cytometric analysis and sorting including instrumentation, technical support, training and consultation.

Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence. Any suspended particle or cell from 0.2–150 micrometers in size is suitable for analysis. Cells from solid tissue must be disaggregated before analysis. Sorting allows us to capture and collect cells of interest for further analysis. Once collected, the cells can be analyzed microscopically, biochemically, or functionally.

To learn more, view Invitrogen's Introduction to Flow Cytometry tutorial.

Location

FACS Facility is located:

  • The 6th floor of The Anlyan Center for Medical Research
    (TAC rooms S613, 617, 633, 533, 522)
  • Amistad building (in rooms 105 and 416)
  • LEPH (in room 901B)
  • Room 2320H at 300 George Street.

FACS Facility Announcements

  • New FACSAriaII is accepting the sorts
  • Updated Facility Rules for Analyzers and Sorters 
  • Stratedigm 8 moved to TAC S613