The PTI-QuantaMaster

The PTI-QuantaMaster can be used for many steady-state applications including DNA binding, Fret, and fluorescence kinetics. This is a very sensitive instrument, capable of gathering emission and excitation spectra as well as time based single wavelength measurements. The tunable Xenon Arc lamp is capable of excitation at any wavelength between 250-650nm. This instrument is also capable of performing lifetime measurements, with the use of LED excitation sources (295, 340, or 525nm). If planning on taking lifetime measurements please consult with the Sweasy lab well in advance of the experiment. Please bring your own cuvettes.

See http://www.pti-nj.com/QuantaMaster-SteadyState.html for more details.

Contact Information:

Daria Sizova 
Hunter Radiation Therapy
15 York Street (Room WWW 308B)
New Haven, CT 06510
Phone: 203-785-4441 
email: daria.sizova@yale.edu

Jamie Towle-Weicksel
Hunter Radiation Therapy
15 York Street (Room WWW 308)
New Haven, CT 06510
Phone: 203-785-4441 
email: jamie.towle-weicksel@yale.edu

Publications

  1. Joyce CM, Potapova O, Delucia AM, Huang X, Basu VP, Grindley ND. (2008) Fingers-closing and other rapid conformational changes in DNA polymerase I (Klenow fragment) and their role in nucleotide selectivity. Biochemistry. 47(23):6103-16.
  2. Li J, Leschka S, Rutschow S, Schwimmbeck PL, Husmann L, Noutsias M, Westermann D, Poller W, Zeichhardt H, Klingel K, Tschope C, Schultheiss HP, Pauschinger M. (2007) Immunomodulation by interleukin-4 suppresses matrix metalloproteinases and improves cardiac function in murine myocarditis. Eur J Pharmacol. 554(1):60-8.
  3. Zhang H, Cao W, Zakharova E, Konigsberg W, De La Cruz EM. (2007) Fluorescence of 2-aminopurine reveals rapid conformational changes in the RB69 DNA polymerase-primer/template complexes upon binding and incorporation of matched deoxynucleoside triphosphates. Nucleic Acids Res. 35(18):6052-62.