Light Scattering Service

The new service offers the exciting capability of determining the native molecular weights of proteins, their protein:protein and other macromolecular complexes. The new service offers the exciting capability of determining the native molecular weights of proteins, their protein:protein and other macromolecular complexes. The MW determination depends only upon the downstream light scattering (LS) and refractive index (RI) detectors, and is thus completely independent of elution position. Hence, non globular shape or possible interaction with the size exclusion chromatography (SEC) support, which otherwise pose severe limitations with regard to the use of SEC for estimating MW, have no impact on the molecular weights determined by LS. Based on analyzing 15 protein standards that range from 6.5 kDa to 475 kDa, native MW’s may be determined quickly and with an error of less than 10%.

DAWN HELEOS II multiangle laser light scattering detector and new refractive index detector: OPTILab rEX.

The second SEC/LS system is capable of determination of MW for a 40 kDa protein at the concentration from ~1 ug/ml to ~20 mg/ml.

Variable temperature option (from 4^oC to 50^oC) coming in June.

Eclipse 3: a state-of-the-art separation system that uses the asymmetric flow Field Flow Fractionation (AFFF) technique.