BD FACSAria at George St
The 300 George St. FACSAria is located in room 203B/2320H. The 18-parameter, 5-laser system includes:
- 100mw 355nm UV laser with 2 PMTs
- 200mw 405nm Violet laser with 6 PMTs
- 200mw 488nm Blue laser with 3 PMTs
- 200mw 532nm Green laser with 4 PMTs
- 150mw 637nm Red laser with 3 PMTs
It can handle UV excitation for cell-cycle and “side-population”-based sorting.
FACSAria II is capable of sorting with 70, 85, 100, and 130 micron nozzles and equipped with FACSDiva 7, which allows for index plate sorting. It also has an aerosol management system and is capable of conducting BSL-1 and BSL-2 sorts. To schedule a sort, or if you have any questions, please contact Jennifer.Kelly@yale.edu or Diane.Trotta@yale.edu.
UV Laser - 355 nm, 100 mW
| PMT | Bandpass | Longpass | Fluorochrome |
| A | 675/50 | 635LP | Hoechst, Side Population |
| B | 450/50 | Indo-1 (Violet), DAPI, Alexa Fluor 350, or BUV395 with 379/28 BP filter
|
Violet Laser - 405 nm, 200 mW
|
Blue Laser -488nm, 200 mW
| PMT |
Bandpass
|
Long Pass
|
|
Fluorochrome |
| A | 710/40 | 685LP |
|
PerCP-Cy5.5, PI(with PE)
|
| B | 582/15 | 555LP |
|
PE, PI
|
| C | 525/50 | 505LP |
|
FITC, AlexaFluor488, CFSE, GFP, YFP |
|
|
488/10 |
|
|
Green Laser - 532 nm, 200 mW
|
Red Laser - 640nm, 150 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/60 | 750LP | APC-Cy7, Alexa Fluor 750, Alexa Fluor 780 |
| B | 730/45 | 685LP | APC-Cy5.5, Alexa Fluor 700 |
| C | 670/30 | APC, Alexa Fluor 647 |