Dermatology; Epithelial Cells; Molecular Biology; Regeneration; Skin Diseases; Stem Cells; Tissue Survival
Postnatal Development & Regeneration
Stem Cell Center, Yale: Stem Cell Niche and Homing | Tissue Specific Stem Cells
Our laboratory studies the cellular and molecular mechanisms that control stem cell activity and function within epithelia, the tissues that line our internal organs and outer surfaces. We use the mouse as a genetic model system to study how adult stem cells within epithelial tissues maintain tissue homeostasis, wound healing and can contribute to cancer formation. The primary epithelial tissue we use is the mammalian skin, which contains multiple stem cell populations and forms a complex tissue that protects our bodies from external pathogens and loss of internal bodily fluids. Mammalian skin is an excellent model to study developmental and stem cell biology because the epidermis and its appendages are in a constant state of regeneration, which is actively sustained by tissue stem cells.
Our vision is to understand the cellular and molecular regulation of epithelial tissue development and regeneration. We focus on both the intrinsic transcriptional as well as the extrinsic signaling mechanisms that control these processes.
- V. Horsley, A. O. Aliprantis, L. Polak, L.H.Glimcher and E. Fuchs. (2008). NFATc1 balances quiescence and proliferation of skin stem cells. Cell. 132(2):299-310.
- V. Horsley, D. O’Carroll, R. Tooze, Y. Ohinata, M.Saitou, T. Obukhanych, M. Nussenzweig, A. Tarakhovsky and E. Fuchs. (2006). Blimp1 defines a novel progenitor population that governs cellular input to the sebaceous gland. Cell. 126(3):597-609.
- E. Festa, J. Fretz, R. Berry, B. Schmidt, M. Rodeheffer, M. Horowitz and V. Horsley. (2011) Adipocyte precursor cells contribute to the skin stem cell niche. Cell. 146(5): 761-771.
- B. Schmidt and V. Horsley. (2013) Intradermal adipocytes regulate fibroblast function during skin wound healing. Development. 140(7): 1517-27.
- A. F. Mertz, Y. Che, S. Banerjee, J. Goldstein, S. Revilla, C. Niessen, M. C. Marchetti, E. R. Dufresne and V. Horsley (2013). Intercellular adhesions organize epithelial cell-matrix mechanics. PNAS. 110(3):842-7.
- A. Tadeu, S. Lin, L. Hou, L. Chung, M. Zhong, H. Zhoa and V. Horsley. (2015) Transcriptional profiling of keratinocyte specification in human embryonic stem cells. Plos One. 10: e0122493.
- K. Rosowski, A. Mertz, S. Norcross, E. Dufresne, and V. Horsley. (2015) Edges of human embryonic stem cell colonies display distinct mechanical properties and differentiation potential. Sci. Rep. 5: 14218.
- R. Stewart, A. Zubek, K. Rosowski, S. Schreiner, V. Horsley*, and M. King* (*co-corresponding author). (2015) LINC complexes support hair follicle integrity and intercellular adhesion. J. Cell Biol. 209: 403-18.
- J. Goldstein, E. Roth, N. Roberts, R. Zwick, S. Lin, S. Fletcher, A. Tadeu, C. Wu, A. Beck, C. Zeiss, M. Suarez-Farinas and V. Horsley. (2015) Loss of endogenous Nfatc1 reduces the rate of DMBA/TPA-induced skin tumorigenesis. Mol. Biol. Cell. pii: mbc.E15-05-0282.
- G. C. Rivera-Gonzalez, B.A. Shook, B. Holtrup, K. Bollag, M. S. Rodeheffer and V. Horsley. (2016) Adipocyte stem cell self-renewal is regulated by a Pdgfa/Akt signalling axis in the skin. Cell Stem Cell. In Press.