Services Offered
We offer the following services. These rates will go into effect beginning fiscal year 2024.
To place a service request, please fill out our Qualtrics form.
All equipment and services are located in Sterling Hall of Medicine IG43.
| Service Description | Internal User Rate | External User Rate (Non-profit) | External User Rate (For-profit) |
|---|---|---|---|
| Making a New Fish Line: Includes design, up to 4 CRISPR constructs, establishing F1 generation, confirming genotype of the new line; deliverable is one frame shift allele; additional alleles are available at a discounted rate | $8,970/line | $13,000/line | $13,000/line |
| Phenotyping Group 1: CRISPR injections to generate F0 mutants for phenotype screening : including design, assembly, and validation; Tol2 transgenic: first step only, including design, assembly, and validation of the vector; Conducting one experiment (control and experimental group) using one construct; Conducting mRNA injection screen, up to 3 constructs/project with 4 fish line developed (Select One) | $1,250/item selected | $2,000/item selected | $2,000/item selected |
| Phenotyping Group 2: Immunohistochemistry, including sample preparation and imaging up to 12 samples; In-situ hybridization, including riboprobe synthesis, staining and imaging up to 12 samples; In-situ hybridization, including staining and imaging up to 12 samples (Select One) | $715/per 12 samples of the selected options | $1,050/per 12 samples of the selected options | |
| Phenotyping: In-situ hybridization, including RNA isolation, gene cloning, riboprobe synthesis, staining, and imaging up to 12 samples | $175/per 12 samples of one gene | $275/per 12 samples of one gene | |
| Phenotyping: Imaging up to 10 fish, including analysis | $525/experiment | $800/experiment | |
| Behavioral Phenotyping: per hour up to 4 hr; >4 hr experiment will be a discount rate | $70/hour | $125/hour | |
| Phenotyping: Monitoring up to 10 tanks for a month, including breeding for F2 generations | $160/per month | $250/per month | $250/per month |
| Genotyping: including tissue harvest and PCR | $35/fish | $75/fish | $75/fish |
| In-vitro Fertilization: Rescuing fish lines | $160/line | ||
| Basic Training: 2 hr session | $400/session | ||
| Consultation: Project feasibility; initial hour free | $250/hour | $500/hour | $500/hour |
| Protocol, Grant, and Manuscript Assistance | $650/per task | $1,000/per task | |
| Using Zebrafish Core Equipment Unassisted; hourly rate | $50/hour | ||
| Cryopreservation: Per fish line | $250/line | $400/line |