2000
Parathyroid hormone-related protein induces spontaneous osteoclast formation via a paracrine cascade
Nakchbandi I, Weir E, Insogna K, Philbrick W, Broadus A. Parathyroid hormone-related protein induces spontaneous osteoclast formation via a paracrine cascade. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 7296-7300. PMID: 10829073, PMCID: PMC16539, DOI: 10.1073/pnas.110553397.Peer-Reviewed Original ResearchConceptsNumber of osteoclastsEffects of PTHrPOsteoclast formationDF cellsBone resorptionFactor expressionOsteoclastic bone resorptionDental follicle cellsHormone-related proteinOsteoclast differentiation factorStellate reticulum cellsOsteoclastogenesis inhibitory factorCoronal tooth surfacesSpontaneous osteoclast formationParacrine cascadeParathyroid hormoneEruption pathwayReticulum cellsPeripheral skeletonTooth eruptionOsteoclast precursorsPTH/PTHrP.Inhibitory factorFunctional osteoclastsCrypt cells
1999
Regulation of parathyroid hormone-related protein gene expression in murine keratinocytes by E1A isoforms: a role for basal promoter and Ets-1 site
Foley J, Wysolmerski J, Missero C, King C, Philbrick W. Regulation of parathyroid hormone-related protein gene expression in murine keratinocytes by E1A isoforms: a role for basal promoter and Ets-1 site. Molecular And Cellular Endocrinology 1999, 156: 13-23. PMID: 10612419, DOI: 10.1016/s0303-7207(99)00151-3.Peer-Reviewed Original ResearchMeSH KeywordsAdenovirus E1A ProteinsAnimalsBase SequenceBinding SitesCell Line, TransformedEnhancer Elements, GeneticGene Expression RegulationGenes, rasGenes, ReporterKeratinocytesLuciferasesMiceParathyroid HormoneParathyroid Hormone-Related ProteinPromoter Regions, GeneticProtein IsoformsProteinsProto-Oncogene Protein c-ets-1Proto-Oncogene ProteinsProto-Oncogene Proteins c-etsRNA, MessengerTranscription FactorsTranscription, GeneticConceptsGene expressionBasal promoterReporter constructsEts-1 siteMinimal promoter regionProtein gene expressionTransient transfection assaysReporter gene activityRepressor domainE1A oncoproteinGene activityRas transformationE1A isoformsMurine keratinocyte lineSite upstreamReporter geneTransfection assaysPromoter regionParathyroid hormone-related protein gene expression
1998
PTHrP Regulates Epidermal Differentiation in Adult Mice
Foley J, Wysolmerski J, Dreyer B, Broadus A, Philbrick W, Longely B. PTHrP Regulates Epidermal Differentiation in Adult Mice. Journal Of Investigative Dermatology 1998, 111: 1122-1128. PMID: 9856827, DOI: 10.1046/j.1523-1747.1998.00428.x.Peer-Reviewed Original ResearchConceptsPTHrP-knockout miceKeratin 14 promoterAdult miceSebaceous glandsHuman keratin 14 promoterParathyroid hormone-related peptideHyperplastic sebaceous glandsHormone-related peptideAbsence of PTHrPNumber of organsWk of agePremature acquisitionEpidermal proliferation rateMarked acanthosisTransgenic replacementReciprocal findingsTransgenic micePTHrPPTHrP geneMiceMammary glandBasal keratinocytesDisplay abnormalitiesLethal chondrodystrophyFibrotic dermisParathyroid hormone-related protein is required for tooth eruption
Philbrick W, Dreyer B, Nakchbandi I, Karaplis A. Parathyroid hormone-related protein is required for tooth eruption. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 11846-11851. PMID: 9751753, PMCID: PMC21728, DOI: 10.1073/pnas.95.20.11846.Peer-Reviewed Original ResearchConceptsPTHrP-knockout miceTooth eruptionEruption pathwayPTHrP expressionType I PTH/PTHrP receptorEnamel epitheliumPTH/PTHrP receptorOverexpression of PTHrPHormone-related proteinMonths of ageNormal tooth developmentParathyroid hormoneBone resorptionPTHrP receptorPTHrP mRNAKnockout miceTransgenic miceAlveolar boneDental mesenchymeLevel of expressionMiceBone formationSkeletal abnormalitiesTooth developmentKeratin 14Parathyroid hormone‐related protein is a developmental regulatory molecule
Philbrick W. Parathyroid hormone‐related protein is a developmental regulatory molecule. European Journal Of Oral Sciences 1998, 106: 32-37. PMID: 9541200, DOI: 10.1111/j.1600-0722.1998.tb02150.x.Peer-Reviewed Original ResearchConceptsDevelopmental regulatory moleculeOverexpression of PTHrPHormone-related proteinHormone-related peptideSingle G protein-coupled receptorG protein-coupled receptorsProtein-coupled receptorsPTHrP overexpressionHumoral hypercalcemiaTumor productsPTHrP functionsKnockout miceMammary epithelial cellsTransgenic miceRegulatory moleculesMiceEpithelial cellsPTH geneRegulatory effectsPTHrPN-terminal productType IMultiple tissuesConvincing evidenceOverexpression
1997
Bcl-2 Lies Downstream of Parathyroid Hormone–related Peptide in a Signaling Pathway That Regulates Chondrocyte Maturation during Skeletal Development
Amling M, Neff L, Tanaka S, Inoue D, Kuida K, Weir E, Philbrick W, Broadus A, Baron R. Bcl-2 Lies Downstream of Parathyroid Hormone–related Peptide in a Signaling Pathway That Regulates Chondrocyte Maturation during Skeletal Development. Journal Of Cell Biology 1997, 136: 205-213. PMID: 9008714, PMCID: PMC2132464, DOI: 10.1083/jcb.136.1.205.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBcl-2-Associated X ProteinBone DevelopmentCartilageCells, CulturedCollagenGene ExpressionGrowth PlateHumansHypertrophyMiceMice, KnockoutMice, TransgenicOrgan SpecificityParathyroid Hormone-Related ProteinPromoter Regions, GeneticProteinsProto-Oncogene ProteinsProto-Oncogene Proteins c-bcl-2Recombinant Fusion ProteinsSignal TransductionConceptsSkeletal developmentChondrocyte maturationBcl-2Cell deathCollagen II promoterApoptotic cell deathMutant PTH/PTHrP receptorsDevelopmental programMolecular mechanismsSignaling pathwaysCell typesGrowth plate chondrocytesTargeted disruptionGenesMetaphyseal chondrodysplasiaMaturationPTHrP geneTransgenic micePathwayPTH/PTHrP receptorAccelerated maturationSkeletal dysplasiaHormone-related peptideMajor roleChondrocytes
1996
Targeted overexpression of parathyroid hormone-related peptide in chondrocytes causes chondrodysplasia and delayed endochondral bone formation.
Weir E, Philbrick W, Amling M, Neff L, Baron R, Broadus A. Targeted overexpression of parathyroid hormone-related peptide in chondrocytes causes chondrodysplasia and delayed endochondral bone formation. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 10240-10245. PMID: 8816783, PMCID: PMC38368, DOI: 10.1073/pnas.93.19.10240.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone DevelopmentDwarfismGene Expression Regulation, DevelopmentalGrowth PlateHumansMiceMice, Inbred C57BLMice, Inbred StrainsMice, TransgenicOsteochondrodysplasiasParathyroid HormoneParathyroid Hormone-Related ProteinPolymerase Chain ReactionProcollagenPromoter Regions, GeneticProtein BiosynthesisProteinsRegulatory Sequences, Nucleic AcidTranscription, GeneticConceptsPTH/PTHrP receptorHormone-related peptideParathyroid hormonePTHrP receptorParathyroid hormone-related peptideChondrocyte differentiationOverexpression of PTHrPType II collagen promoterEndochondral ossificationShort-limbed dwarfismParaneoplastic hypercalcemiaPTHrP functionsBone collar formationMalignant tumorsDevelopmental regulatory moleculeEndochondral bone formationTransgenic micePTHrPCommon receptorEndochondral bone developmentTargeted overexpressionFetal tissuesPTHrP geneFollicle formationNormal boneOverexpression of Parathyroid Hormone-related Protein in the Pancreatic Islets of Transgenic Mice Causes Islet Hyperplasia, Hyperinsulinemia, and Hypoglycemia (∗)
Vasavada R, Cavaliere C, D'Ercole A, Dann P, Burtis W, Madlener A, Zawalich K, Zawalich W, Philbrick W, Stewart A. Overexpression of Parathyroid Hormone-related Protein in the Pancreatic Islets of Transgenic Mice Causes Islet Hyperplasia, Hyperinsulinemia, and Hypoglycemia (∗). Journal Of Biological Chemistry 1996, 271: 1200-1208. PMID: 8557651, DOI: 10.1074/jbc.271.2.1200.Peer-Reviewed Original ResearchConceptsRat insulin II promoterInsulin secretionIslet massPancreatic isletsNormal littermatesHormone-related proteinBlood glucose valuesTotal islet massIslet perifusion studiesNormal physiologic roleInappropriate hyperinsulinemiaParathyroid hormoneIslet hyperplasiaInsulin concentrationsTotal islet numberAutocrine roleGlucose homeostasisPerifusion studiesTransgenic miceGlucose valuesIslet numberIslet cellsPhysiologic rolePTHrPMice
1995
Further evidence for a novel receptor for amino-terminal parathyroid hormone-related protein on keratinocytes and squamous carcinoma cell lines
Orloff J, Kats Y, Urena P, Schipani E, Vasavada R, Philbrick W, Behal A, Abou-Samra A, Segre G, Jüppner H. Further evidence for a novel receptor for amino-terminal parathyroid hormone-related protein on keratinocytes and squamous carcinoma cell lines. Endocrinology 1995, 136: 3016-3023. PMID: 7789327, DOI: 10.1210/endo.136.7.7789327.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBlotting, NorthernCarcinoma, Squamous CellCyclic AMPDNA ProbesGene ExpressionHumansKeratinocytesMolecular Sequence DataParathyroid HormoneParathyroid Hormone-Related ProteinPeptide FragmentsProteinsRadioligand AssayReceptor, Parathyroid Hormone, Type 1Receptors, Parathyroid HormoneRNA, MessengerTransfectionTumor Cells, CulturedConceptsSquamous carcinoma cell linesSquamous cell linesPTH/PTHrP receptorType I receptorCarcinoma cell linesI receptorSquamous cellsPTHrP receptorCell linesCommon PTH/PTHrP receptorParathyroid hormone-related proteinType INormal keratinocytesAmino-terminal PTHMessenger RNAHormone-related proteinReceptor messenger RNAPTH target tissuesIntracellular cAMP responseSqCC/Y1 cellsSquamous carcinoma cellsStrong RNA signalsReceptor complementary DNAType II receptorIntracellular calciumCell Type-specific Secretion of Parathyroid Hormone-related Protein via the Regulated versus the Constitutive Secretory Pathway∗
Plawner L, Philbrick W, Burtis W, Broadus A, Stewart A. Cell Type-specific Secretion of Parathyroid Hormone-related Protein via the Regulated versus the Constitutive Secretory Pathway∗. Journal Of Biological Chemistry 1995, 270: 14078-14084. PMID: 7775469, DOI: 10.1074/jbc.270.23.14078.Peer-Reviewed Original ResearchConceptsNon-neuroendocrine cellsConstitutive pathwayRegulated pathwayCell typesRegulated secretory pathwayNeuroendocrine cellsNeuroendocrine cell typesDaughter peptidesSecretory pathwayRegulated fashionMature secretory formsConstitutive fashionSecretory formPathwayHormone-related proteinProteinCellsPolypeptide hormonesPathway usePeptidesParathyroid hormone-related proteinSecretion of PTHrPSecretionSecretory