2022
RAGE antagonism with azeliragon improves xenograft rejection by T cells in humanized mice.
Joshi AA, Wu Y, Deng S, Preston-Hurlburt P, Forbes JM, Herold KC. RAGE antagonism with azeliragon improves xenograft rejection by T cells in humanized mice. Clinical Immunology 2022, 245: 109165. PMID: 36257528, DOI: 10.1016/j.clim.2022.109165.Peer-Reviewed Original ResearchConceptsXenograft rejectionIL-17AHumanized miceIL-1βT cellsImmune responseRAGE antagonistsAdaptive human immune responsesPD-1 expressionSkin graft rejectionHuman immune cell responsesImmune cell responsesHuman immune responseHuman immune cellsInnate immune responseAdvanced glycation endproductsInhibition of pathwaysSmall molecule antagonistsMultiple inflammatory processesAZ therapyRAGE antagonismGraft rejectionIL-23Serum levelsMedian timeImmune cells and their inflammatory mediators modify beta cells and cause checkpoint inhibitor-induced diabetes
Perdigoto AL, Deng S, Du KC, Kuchroo M, Burkhardt DB, Tong A, Israel G, Robert ME, Weisberg SP, Kirkiles-Smith N, Stamatouli AM, Kluger HM, Quandt Z, Young A, Yang ML, Mamula MJ, Pober JS, Anderson MS, Krishnaswamy S, Herold KC. Immune cells and their inflammatory mediators modify beta cells and cause checkpoint inhibitor-induced diabetes. JCI Insight 2022, 7: e156330. PMID: 35925682, PMCID: PMC9536276, DOI: 10.1172/jci.insight.156330.Peer-Reviewed Original ResearchConceptsCheckpoint inhibitorsΒ-cellsPD-1/PD-L1 pathwayT-lymphocyte antigen-4PD-1 blockadePD-L1 pathwayDeath ligand 1NOD mouse modelDevelopment of diabetesHuman β-cellsAutoimmune complicationsNOD miceΒ-cell populationDeath-1Diabetes mellitusImmune infiltratesInflammatory mediatorsPancreatic inflammationPD-L1Induced diabetesLymphocytic infiltrationInflammatory cytokinesAntigen-4Immune cellsT cells
2017
Microbiota control immune regulation in humanized mice
Gülden E, Vudattu NK, Deng S, Preston-Hurlburt P, Mamula M, Reed JC, Mohandas S, Herold BC, Torres R, Vieira SM, Lim B, Herazo-Maya JD, Kriegel M, Goodman AL, Cotsapas C, Herold KC. Microbiota control immune regulation in humanized mice. JCI Insight 2017, 2: e91709. PMID: 29093268, PMCID: PMC5752290, DOI: 10.1172/jci.insight.91709.Peer-Reviewed Original ResearchMeSH KeywordsAdaptive ImmunityAnimalsAntibodies, AntinuclearAntibodies, Monoclonal, HumanizedAutoimmune DiseasesB7-2 AntigenCD11b AntigenCD11c AntigenCD3 ComplexCD8-Positive T-LymphocytesCytokinesDisease Models, AnimalGastrointestinal MicrobiomeGastrointestinal TractGraft RejectionHumansImmunosuppressive AgentsImmunotherapyInterferon-gammaInterleukin-10Interleukin-27Leukocytes, MononuclearMiceMice, KnockoutMucous MembraneSkin TransplantationSTAT5 Transcription FactorT-LymphocytesTransplantation, HeterologousConceptsT cellsIL-10Humanized miceHuman peripheral blood mononuclear cellsPeripheral blood mononuclear cellsIL-27 expressionIL-10 levelsAnti-nuclear antibodiesEffector T cellsLevels of IFNCentral memory cellsLess IL-10Markers of efficacyBlood mononuclear cellsExpression of CD86Immune regulatory pathwaysIL-10 inductionHuman immune cellsHuman stool samplesImmunosuppressive medicationsIL-27Xenograft rejectionImmune therapyMononuclear cellsAntibiotic treatmentOral treatment with foralumab, a fully human anti-CD3 monoclonal antibody, prevents skin xenograft rejection in humanized mice
Ogura M, Deng S, Preston-Hurlburt P, Ogura H, Shailubhai K, Kuhn C, Weiner HL, Herold KC. Oral treatment with foralumab, a fully human anti-CD3 monoclonal antibody, prevents skin xenograft rejection in humanized mice. Clinical Immunology 2017, 183: 240-246. PMID: 28739191, DOI: 10.1016/j.clim.2017.07.005.Peer-Reviewed Original ResearchConceptsSkin xenograft rejectionOral treatmentXenograft rejectionT cellsAnti-CD3 monoclonal antibodyConsecutive daily dosesPeripheral T cellsActivation of splenocytesHuman immune systemSplenic CD8Graft acceptanceWeekly dosingIL-10Serum levelsImmune therapySmall bowelHumanized miceDaily dosesImmune modulationMucosal barrierIntragastric doseOral administrationSkin graftsProliferative responseLymphoid cells
2016
Methylation of insulin DNA in response to proinflammatory cytokines during the progression of autoimmune diabetes in NOD mice
Rui J, Deng S, Lebastchi J, Clark PL, Usmani-Brown S, Herold KC. Methylation of insulin DNA in response to proinflammatory cytokines during the progression of autoimmune diabetes in NOD mice. Diabetologia 2016, 59: 1021-1029. PMID: 26910463, PMCID: PMC4826795, DOI: 10.1007/s00125-016-3897-4.Peer-Reviewed Original ResearchConceptsInsulin gene expressionGene expressionQuantitative real-time RT-PCRMethylation marksInsulin geneNOD miceBeta cellsDNA methyltransferasesDisease progressionAims/hypothesisType 1 diabetesIns2 geneInsulin DNAMethylationReal-time RT-PCRGenesBeta-cell functionExon 1Human beta cellsBeta-cell massPancreatic beta cellsType 1 diabetesMethyltransferasesEffects of cytokinesExon 2Cell mass
2015
Sodium chloride inhibits the suppressive function of FOXP3+ regulatory T cells
Hernandez AL, Kitz A, Wu C, Lowther DE, Rodriguez DM, Vudattu N, Deng S, Herold KC, Kuchroo VK, Kleinewietfeld M, Hafler DA. Sodium chloride inhibits the suppressive function of FOXP3+ regulatory T cells. Journal Of Clinical Investigation 2015, 125: 4212-4222. PMID: 26524592, PMCID: PMC4639983, DOI: 10.1172/jci81151.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAnimalsAntibodies, NeutralizingAutoimmunityCD4-Positive T-LymphocytesCells, CulturedCoculture TechniquesColitisCytokinesForkhead Transcription FactorsGene Expression ProfilingGenes, ReporterGraft vs Host DiseaseHeterograftsHumansImmediate-Early ProteinsInflammationInterferon-gammaLeukocytes, MononuclearMaleMiceProtein Serine-Threonine KinasesRNA InterferenceRNA, Small InterferingSodium ChlorideSodium Chloride, DietaryT-Lymphocytes, RegulatoryConceptsHigh-salt dietTreg functionIFNγ secretionCD4 effector cellsHuman Treg functionRegulatory T cellsAdoptive transfer modelAnti-IFNγ antibodyHost disease modelType 1 diabetesInduction of proinflammatoryTreg pathwayExperimental colitisXenogeneic graftEffector cellsMultiple sclerosisProinflammatory responseT cellsTregsMurine modelSuppressive activitySuppressive functionSerum/glucocorticoid-regulated kinaseAutoimmunityGlucocorticoid-regulated kinase
2014
CD45 ligation expands Tregs by promoting interactions with DCs
Camirand G, Wang Y, Lu Y, Wan YY, Lin Y, Deng S, Guz G, Perkins DL, Finn PW, Farber DL, Flavell RA, Shlomchik WD, Lakkis FG, Rudd CE, Rothstein DM. CD45 ligation expands Tregs by promoting interactions with DCs. Journal Of Clinical Investigation 2014, 124: 4603-4613. PMID: 25202978, PMCID: PMC4191025, DOI: 10.1172/jci74087.Peer-Reviewed Original ResearchConceptsT cellsTreg proliferationTreg expansionVivo expansionRegulatory T cellsConventional T cellsTransfer of TregsActivated T cellsTreg numbersTreg populationImmune toleranceHomeostatic proliferationWT miceExogenous antigensPeripheral homeostasisTregsImmune responseTherapeutic useCD45 ligationNuclear factorIntegrin-dependent mannerNuclear translocationLigationAntigenProliferationHumanized Mice as a Model for Aberrant Responses in Human T Cell Immunotherapy
Vudattu NK, Waldron-Lynch F, Truman LA, Deng S, Preston-Hurlburt P, Torres R, Raycroft MT, Mamula MJ, Herold KC. Humanized Mice as a Model for Aberrant Responses in Human T Cell Immunotherapy. The Journal Of Immunology 2014, 193: 587-596. PMID: 24943216, PMCID: PMC4123131, DOI: 10.4049/jimmunol.1302455.Peer-Reviewed Original ResearchMeSH KeywordsAdrenal GlandsAnimalsAntibodies, MonoclonalAntibodies, Monoclonal, HumanizedAutoimmune DiseasesCytokinesDisease Models, AnimalFlow CytometryHumansInterleukin Receptor Common gamma SubunitIpilimumabLiverLymphocyte ActivationMacrophagesMiceMice, Inbred NODMice, KnockoutMice, SCIDPhosphorylationSTAT5 Transcription FactorStem Cell TransplantationSurvival AnalysisT-LymphocytesT-Lymphocytes, RegulatoryTransplantation, HeterologousWeight LossConceptsAnti-nuclear AbsAutoimmune diseasesRegulatory cellsHumanized miceT cellsImmune responseWeight lossMesenteric lymph nodesHuman autoimmune diseasesInduction of autoimmunityT-cell immunotherapyRelease of IFNHuman immune responseImmune-deficient miceIpilimumab treatmentInflammatory sequelaeLymph nodesCell immunotherapyIP-10Macrophage infiltrationCytokine productionSpleen cellsPathologic processesHepatitisMice
2013
Transplant Tolerance to Pancreatic Islets Is Initiated in the Graft and Sustained in the Spleen
Gagliani N, Jofra T, Valle A, Stabilini A, Morsiani C, Gregori S, Deng S, Rothstein DM, Atkinson M, Kamanaka M, Flavell RA, Roncarolo MG, Battaglia M. Transplant Tolerance to Pancreatic Islets Is Initiated in the Graft and Sustained in the Spleen. American Journal Of Transplantation 2013, 13: 1963-1975. PMID: 23834659, PMCID: PMC3869180, DOI: 10.1111/ajt.12333.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAnimalsAntibodies, MonoclonalCD4 AntigensCD4-Positive T-LymphocytesForkhead Transcription FactorsGraft SurvivalIslets of LangerhansIslets of Langerhans TransplantationLeukocyte Common AntigensMiceMice, Inbred BALB CMice, Inbred C57BLSpleenT-Lymphocytes, RegulatoryTransplantation ToleranceTransplantation, HomologousConceptsTr1 cellsLong-term toleranceTransplant toleranceRegulatory type 1 (Tr1) cellsSpecific immunomodulatory treatmentTreg cell transferCD25- T cellsRegulatory cell typesType 1 cellsAllograft toleranceImmunomodulatory treatmentTreg cellsAllogeneic transplantationT cellsMouse modelImmune systemTregsPancreatic isletsSpleenAllograftsCell typesCellsRegulatory functionsTransplantationEngraftment
2012
Analysis of Human Biologics With a Mouse Skin Transplant Model in Humanized Mice
Waldron-Lynch F, Deng S, Preston-Hurlburt P, Henegariu O, Herold KC. Analysis of Human Biologics With a Mouse Skin Transplant Model in Humanized Mice. American Journal Of Transplantation 2012, 12: 2652-2662. PMID: 22900715, DOI: 10.1111/j.1600-6143.2012.04178.x.Peer-Reviewed Original ResearchConceptsSkin transplant modelGraft rejectionTransplant modelHumanized miceSkin graftsT cellsFunctional human immune responsesMouse skin transplant modelMurine skin transplant modelCentral memory T cellsNOD/SCID/Mouse skin graftsMemory T cellsMonoclonal antibody therapySkin graft rejectionDevelopment of effectorHuman immune responseMHC class IHuman T cellsIpilimumab treatmentAntibody therapySCID/Diffuse infiltrationMouse donorsSerum immunoglobulinsTeplizumab Induces Human Gut-Tropic Regulatory Cells in Humanized Mice and Patients
Waldron-Lynch F, Henegariu O, Deng S, Preston-Hurlburt P, Tooley J, Flavell R, Herold KC. Teplizumab Induces Human Gut-Tropic Regulatory Cells in Humanized Mice and Patients. Science Translational Medicine 2012, 4: 118ra12. PMID: 22277969, PMCID: PMC4131554, DOI: 10.1126/scitranslmed.3003401.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, Monoclonal, HumanizedCD3 ComplexCell MovementDiabetes Mellitus, Type 1Forkhead Transcription FactorsGastrointestinal TractHumansHypoglycemic AgentsInterleukin-10Intestine, SmallL-SelectinMiceMucous MembraneNatalizumabOligonucleotide Array Sequence AnalysisReceptors, CCR6T-Lymphocytes, RegulatoryConceptsHumanized micePeripheral circulationSmall intestineType 1 diabetes mellitusNovel immunologic mechanismIL-10 expressionTreatment of patientsType 1 diabetesSecondary lymph organsHuman immune cellsT cell migrationMechanism of actionGut-tropicImmunologic mechanismsRegulatory cellsDiabetes mellitusImmune therapyInterleukin-10Immune cellsRegulatory cytokinesClinical trialsPreclinical modelsClinical studiesT cellsHuman hematopoietic stem cells
2011
Regulatory T Cells Require Mammalian Target of Rapamycin Signaling To Maintain Both Homeostasis and Alloantigen-Driven Proliferation in Lymphocyte-Replete Mice
Wang Y, Camirand G, Lin Y, Froicu M, Deng S, Shlomchik WD, Lakkis FG, Rothstein DM. Regulatory T Cells Require Mammalian Target of Rapamycin Signaling To Maintain Both Homeostasis and Alloantigen-Driven Proliferation in Lymphocyte-Replete Mice. The Journal Of Immunology 2011, 186: 2809-2818. PMID: 21270412, PMCID: PMC5584652, DOI: 10.4049/jimmunol.0903805.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell ProliferationCell SurvivalGene Knock-In TechniquesHomeostasisIsoantigensLymphocyte ActivationLymphocyte CountLymphocyte DepletionLymphoid TissueMiceMice, Inbred BALB CMice, Inbred C57BLSignal TransductionSirolimusSkin TransplantationT-Lymphocytes, RegulatoryTOR Serine-Threonine KinasesConceptsPercent of TregRegulatory T cellsHost disease modelT cellsTolerogenic agentsMammalian targetConversion of TregsEffect of RapaFoxp3(-) T cellsNumber of TregsSolid organ transplantationDisease modelsAlloantigen-induced proliferationWild-type miceRAPA therapyTreg depletionTreg survivalCD4 cellsLymphopenic hostsImmunosuppressive drugsTregsOrgan transplantationAnimal modelsBaseline numberTconv
2008
Anti‐CD20 Treatment Prolongs Syngeneic Islet Graft Survival and Delays the Onset of Recurrent Autoimmune Diabetes
Hu C, Deng S, Wong FS, Wen L. Anti‐CD20 Treatment Prolongs Syngeneic Islet Graft Survival and Delays the Onset of Recurrent Autoimmune Diabetes. Annals Of The New York Academy Of Sciences 2008, 1150: 217-219. PMID: 19120299, DOI: 10.1196/annals.1447.032.Peer-Reviewed Original ResearchConceptsImmunosuppressive drug treatmentType 1 diabetesIslet transplantationDrug treatmentLong-term immunosuppressive drug treatmentSyngeneic islet graft survivalB cell depletion therapyT cell-mediated destructionAccepted therapeutic optionB-cell depletionPancreatic islet beta cellsCell-mediated destructionIslet graft survivalSyngeneic islet transplantationPancreatic islet transplantationIslet beta cellsRecurrent AutoimmuneTolerance establishmentGraft survivalNOD miceTherapeutic optionsAutoimmune diseasesCell depletionInsulin treatmentBeta cells
2006
Antigen Exposure during Enhanced CTLA-4 Expression Promotes Allograft Tolerance In Vivo
Salvalaggio PR, Camirand G, Ariyan CE, Deng S, Rogozinski L, Basadonna GP, Rothstein DM. Antigen Exposure during Enhanced CTLA-4 Expression Promotes Allograft Tolerance In Vivo. The Journal Of Immunology 2006, 176: 2292-2298. PMID: 16455985, DOI: 10.4049/jimmunol.176.4.2292.Peer-Reviewed Original ResearchConceptsCTLA-4 expressionLong-term engraftmentBALB/cCTLA-4Allograft toleranceCD4 cellsAnti-CD45RB treatmentAcute rejectionCD25 depletionDonor AgUnrelated alloantigensPeritransplant periodAntigen exposureEffector cellsPoor outcomeUnrelated AgAg exposureTransplantationEngraftmentDay 17Day 0Therapeutic enhancementMiceAlloantigensTemporal relationship
2005
Testicular Immune Privilege Promotes Transplantation Tolerance by Altering the Balance between Memory and Regulatory T Cells
Nasr IW, Wang Y, Gao G, Deng S, Diggs L, Rothstein DM, Tellides G, Lakkis FG, Dai Z. Testicular Immune Privilege Promotes Transplantation Tolerance by Altering the Balance between Memory and Regulatory T Cells. The Journal Of Immunology 2005, 174: 6161-6168. PMID: 15879112, DOI: 10.4049/jimmunol.174.10.6161.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BlockingApoptosisCD40 AntigensCD40 LigandCD8-Positive T-LymphocytesEpitopes, T-LymphocyteGraft RejectionGrowth InhibitorsImmunologic MemoryIslets of Langerhans TransplantationLymphocyte ActivationLymphocyte CountMaleMiceMice, Inbred BALB CMice, Inbred C3HMice, Inbred C57BLMice, KnockoutMice, TransgenicReceptors, Interleukin-2T-Lymphocytes, RegulatoryTestisTransplantation ToleranceTransplantation, HeterotopicConceptsRegulatory T cellsNon-privileged sitesIslet allograftsTransplantation toleranceT cellsImmune privilegeCD40/CD40L costimulationIntratesticular islet allograftsIslet allograft toleranceAcute allograft rejectionProlong allograft survivalLate allograft failureTesticular immune privilegePrivileged siteAllograft failureAllograft survivalAllograft toleranceCostimulatory blockadeAllograft rejectionAlloimmune responseSkin allograftsIslet transplantationImmune regulationImmune responseAllograftsImpaired Recall of CD8 Memory T Cells in Immunologically Privileged Tissue
Dai Z, Nasr IW, Reel M, Deng S, Diggs L, Larsen CP, Rothstein DM, Lakkis FG. Impaired Recall of CD8 Memory T Cells in Immunologically Privileged Tissue. The Journal Of Immunology 2005, 174: 1165-1170. PMID: 15661869, DOI: 10.4049/jimmunol.174.3.1165.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCD8-Positive T-LymphocytesCell MovementEpitopes, T-LymphocyteFas ReceptorGraft RejectionImmunologic MemoryIslets of Langerhans TransplantationKi-1 AntigenKidneyMaleMiceMice, Inbred BALB CMice, Inbred C57BLMice, Inbred MRL lprMice, KnockoutMice, Mutant StrainsMice, TransgenicTestisTransplantation, HeterotopicConceptsMemory T cellsCD8 memory T cellsT cellsForeign AgsPrivileged tissuesT cell-mediated rejectionCell-mediated rejectionPancreatic islet allograftsDelayed graft rejectionTNFR family membersPrivileged siteIntratesticular graftsGraft rejectionIslet allograftsImmunosuppressive mechanismsImmune privilegeAllogeneic graftsDiabetic miceKidney capsuleSame AgTumor cellsGraftImpaired recallFamily membersCells
2003
Cutting Edge: Transplantation Tolerance through Enhanced CTLA-4 Expression
Ariyan C, Salvalaggio P, Fecteau S, Deng S, Rogozinski L, Mandelbrot D, Sharpe A, Sayegh MH, Basadonna GP, Rothstein DM. Cutting Edge: Transplantation Tolerance through Enhanced CTLA-4 Expression. The Journal Of Immunology 2003, 171: 5673-5677. PMID: 14634073, DOI: 10.4049/jimmunol.171.11.5673.Peer-Reviewed Original ResearchMeSH KeywordsAbataceptAdjuvants, ImmunologicAnimalsAntibodies, MonoclonalAntigens, CDAntigens, DifferentiationCTLA-4 AntigenGraft Enhancement, ImmunologicGraft SurvivalImmunoconjugatesInjections, IntraperitonealInjections, IntravenousIslets of Langerhans TransplantationLeukocyte Common AntigensMaleMiceMice, Inbred BALB CMice, Inbred C57BLMice, KnockoutSignal TransductionTransplantation ToleranceUp-RegulationConceptsCTLA-4 expressionCTLA-4Allograft survivalCTLA4-IgAnti-CD45RB therapyIslet allograft survivalCTLA-4 interactionWild-type miceLong-term engraftmentPeripheral toleranceTransplantation toleranceAllogeneic cellsB7-1B7-2Requisite roleEngraftmentMiceB7SurvivalSpecific roleCritical roleExpressionTransplantationTherapyRecipients
2001
Targeting Signal 1 Through CD45RB Synergizes with CD40 Ligand Blockade and Promotes Long Term Engraftment and Tolerance in Stringent Transplant Models
Rothstein D, Livak M, Kishimoto K, Ariyan C, Qian H, Fecteau S, Sho M, Deng S, Zheng X, Sayegh M, Basadonna G. Targeting Signal 1 Through CD45RB Synergizes with CD40 Ligand Blockade and Promotes Long Term Engraftment and Tolerance in Stringent Transplant Models. The Journal Of Immunology 2001, 166: 322-329. PMID: 11123308, DOI: 10.4049/jimmunol.166.1.322.Peer-Reviewed Original ResearchMeSH KeywordsAdjuvants, ImmunologicAnimalsAntibodies, BlockingAntibodies, MonoclonalB-LymphocytesCD40 LigandCD8-Positive T-LymphocytesCell MovementCytokinesDrug Therapy, CombinationGraft SurvivalIslets of Langerhans TransplantationLeukocyte Common AntigensMaleMiceMice, Inbred BALB CMice, Inbred C57BLModels, ImmunologicalMonocytesProtein IsoformsSignal TransductionSkin TransplantationTransplantation ToleranceConceptsCostimulatory blockadeCD40 ligand blockadeStringent transplant modelSkin allograft survivalBlockade of CD28Induction of toleranceTh2 cytokine expressionLong-term engraftmentImmunogenic allograftAllograft survivalAllograft toleranceAllograft rejectionC57BL/6 recipientsCD8 cellsIslet allograftsTh1 cytokinesTransplant modelCostimulatory pathwayCytokine expressionCD40 ligandMurine modelB cellsTerm engraftmentBlockadeEngraftment