2021
YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusion
Feliciano D, Ott CM, Espinosa-Medina I, Weigel AV, Benedetti L, Milano KM, Tang Z, Lee T, Kliman HJ, Guller SM, Lippincott-Schwartz J. YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusion. Nature Communications 2021, 12: 4502. PMID: 34301937, PMCID: PMC8302681, DOI: 10.1038/s41467-021-24708-2.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAMP-Activated Protein KinasesAnimalsBiological TransportCell FusionCell LineCell Line, TumorCell MembraneCell NucleusCells, CulturedGiant CellsHEK293 CellsHumansMembrane GlycoproteinsMiceRNA-SeqSignal TransductionTranscription FactorsTranscription, GeneticViral Envelope ProteinsYAP-Signaling ProteinsConceptsCell cycle arrestCell fusionNew cellular statesPlasma membrane surface areaRNA-seq analysisCell fusion systemTranscriptional programsNutrient stressCellular statesTranscriptional changesNuclear effluxCytoplasmic glucoseExtrinsic cuesUndifferentiated cellsGlucose transporterFused cellsMechanistic insightsMembrane surface areaNew functionsEndocytosisYAP1 inhibitionEnergetic stateSyncytiaCellsVSV
2017
Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation.
Cross SN, Potter JA, Aldo P, Kwon JY, Pitruzzello M, Tong M, Guller S, Rothlin CV, Mor G, Abrahams VM. Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation. The Journal Of Immunology 2017, 199: 2885-2895. PMID: 28916522, PMCID: PMC5659726, DOI: 10.4049/jimmunol.1700870.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsC-Mer Tyrosine KinaseCells, CulturedChorioamnionitisExtraembryonic MembranesFemaleGammaherpesvirinaeHerpesviridae InfectionsHerpesvirus 2, HumanHumansImmunizationInflammasomesIntercellular Signaling Peptides and ProteinsInterleukin-1betaLipopolysaccharidesMiceMice, Inbred C57BLMice, KnockoutPregnancyPremature BirthProto-Oncogene ProteinsReceptor Protein-Tyrosine KinasesConceptsFetal membranesViral infectionImmune responseTAM receptorsSubsequent preterm birthAdverse pregnancy outcomesIL-1β productionIL-1β responseHerpes viral infectionsInnate immune responseVivo mouse modelHuman fetal membranesAggravated inflammationIL-1β processingPregnancy outcomesPreterm birthAssociated complicationsPremature rupturePregnant womenLocal infectionInflammasome activationMerTK inhibitionMouse modelBacterial LPSLigand expression
2013
Viral Infection of the Pregnant Cervix Predisposes to Ascending Bacterial Infection
Racicot K, Cardenas I, Wünsche V, Aldo P, Guller S, Means RE, Romero R, Mor G. Viral Infection of the Pregnant Cervix Predisposes to Ascending Bacterial Infection. The Journal Of Immunology 2013, 191: 934-941. PMID: 23752614, PMCID: PMC4153356, DOI: 10.4049/jimmunol.1300661.Peer-Reviewed Original ResearchConceptsFemale reproductive tractBacterial infectionsViral infectionLower female reproductive tractReproductive tractAntimicrobial peptide expressionProduction of mucusPreterm laborPreterm birthNeonatal mortalityUterine cavityUterine infectionMouse modelPeptide expressionInfectionCervixPolymicrobial diseaseMajor causeMultifactorial approachCommon routePregnancyBirthUterusTractAntimicrobial peptides
2010
Role of GnRH–GnRH receptor signaling at the maternal-fetal interface
Lee HJ, Snegovskikh VV, Park JS, Foyouzi N, Han KT, Hodgson EJ, Guller S, Norwitz ER. Role of GnRH–GnRH receptor signaling at the maternal-fetal interface. Fertility And Sterility 2010, 94: 2680-2687. PMID: 20400076, DOI: 10.1016/j.fertnstert.2010.03.016.Peer-Reviewed Original ResearchConceptsGnRHR-I mRNAMaternal-fetal interfaceDecidual stromal cellsHCG productionGnRH-IISelect cytokinesTrophoblast cellsCell linesEffects of GnRHUniversity Teaching HospitalExpression of GnRHFunction of GnRHReverse transcription-polymerase chain reactionDose-dependent fashionHuman trophoblast cellsTerm deliveryPolymerase chain reactionTeaching hospitalGnRH receptorMAIN OUTCOMEGnRHHuman trophoblastsStromal cellsWestern blotImmortalized cell lines